Objectives: This study aimed identify of the level and effect of the e-health literacy and health behavior in health science majors Methods: The data was collected from March 5 to March 15, 2018 for student majoring in health science at a university in the C city. The data were analyzed with the SPSS 21.0. Results: First, the e-health literacy were statistically significant in grades (F=5.769, p=0.001), health interest (F=10.553, p<0.001) and health care time (F=3.841, p=0.023), and health behavior were statistically significant in subjective health condition (F=5.476, p=0.05), health interest (F=16.716, p<0.001), and health care time (F=28.479, p<0.001). Second, the e-health literacy were correlated with grades, health care time, economic level and health interest, and health behavior were related to health care time, e-health literacy, subjective health condition and health interest. Third, health behavior related factors were health care time, e-health literacy, grades, subjective health condition, and health interest. Conclusion: The related knowledge and skills should be applied to basic classes in order to ensure that health science majors care can accurately utilize the information on the e-health.