Estimation efficiencies according to different sea trial are investigated in connection with sensitivity analysis, and new trial method is proposed which can improve the estimation efficiency of hydrodynamic derivatives. MMG Equation with Kijima's formula is used for simulation. Extended Kalman Filter is chosen for estimation technique and hydrodynamic derivatives of interest is limited to 12 of those in sway and yaw equations. Esso Osaka is selected for the test ship. Sensitivity analysis and estimation results based on conventional trials show that a more sensitive derivative gives more efficient estimation result. Sensitivities of nonlinear derivatives become pronounced in the trial where steady condition lasts longer such as turning test, while sensitivities of linear derivatives gas a larger values in the trial where unsteady condition lasts longer such as 10deg-10deg zigzag test. Consequently, in new method , named S-type trial, steady and unsteady condition are combined appropriately to increase sensitivities. Linear derivatives are estimated better in S-type trial and the estimation of nonlinear derivatives is improved to extent.