Purpose: This descriptive study aimed to identify shift nurses' retention intention, need satisfaction, calling and vocation, and job stress, as well as the effects of these factors on retention intention. Methods: The participants of this study were 193 nurses working shifts with more than three months' clinical experience at general hospitals. Data were collected using a structured questionnaire from February 1 to 28, 2023. Nurses' Retention Index and Nurse Needs Satisfaction Scale, Korean version of the Calling and Vocational Questionnaire, Korean Occupational Stress Scale were used. Data were analyzed using SPSS/WIN 23.0 program. Independent t-test, one-way ANOVA, Pearson's correlation analysis, and hierarchical multiple regression analysis were conducted for statistical analysis. Results: Regarding nurses' shift work, the mean scores for retention intention, needs satisfaction, calling and vocation, and job stress were 32.12±9.04, 94.87±14.74, 26.95±6.84, and 66.12±10.34. Moreover, the nurses' retention intention differed significantly by age, education level, religion, marital status, clinical experience, satisfaction with wage, and job satisfaction. Furthermore, retention intention was positively correlated with needs satisfaction and calling and vocation and negatively correlated with job stress. Needs satisfaction, and calling and vocation as well as education level of bachelor's degree, job satisfaction had positive effects on shift work nurses' retention intention. Job dissatisfaction had relatively negative effects on retention intention compared to having neutral job satisfaction. Conclusion: It is necessary to develop programs that promote needs satisfaction, calling and vocation, and job satisfaction to improve nurses' retention intention in relation to shift work; moreover, institutional support for acquiring a bachelor's degree or higher is needed.