The purpose of this study was to analyze affecting factors on labor union commitment among nurses in two general hospitals. The subjects of this study were 282 nurses in one university hospital in Seoul and one general hospital in Gyeong-Gi province from April 20 to May 9, 2008 through survey questionnaires. The main results of this study were as follow: First, labor union commitment level among nurses was increased as 30 years old and lower job position. Second, organization conflict level among nurses did not statistical differ significantly regarding age, education level, wedding, job year, job position, and labor manager. Third, correlation between labor union commitment level and other variables as follow. It was increased as age, education level, job year, job position, organization conflict level but not statistical difference significantly. Finally, from the results of multiple regression analysis to identify major affecting factors of labor union commitment level, it depends on low education level, high conflict between individual and group but not significantly. Therefore, new research was required regarding organization culture and commitment.