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방사선 조사 및 Paraquat투여를 한 생쥐 간에 대한 홍삼의 보호 효과

The Protective Effect of Red Ginseng(RG) Extracts on the Liver of Mice by X-ray Irradiation and Medication of Paraquat Orally

  • 고인호 (제주한라대학교 방사선과) ;
  • 여진동 (서라벌대학교 방사선과)
  • Ko, InHo (Dept. of Radiological Technology, Cheju Halla University) ;
  • Yeo, JinDong (Dept. of Radiological Technology, Sarabol University)
  • 투고 : 2014.02.26
  • 심사 : 2014.04.25
  • 발행 : 2014.04.30

초록

방사선과 paraquat에 의해서 유도된 간 손상에 대한 홍삼추출물의 보호효과를 비교 연구하였다. ICR계 생쥐에게 X선의 5Gy조사와 paraquat투여 7일 전부터 홍삼추출물(200mg/kg/day)을 투여하였다. 대조군은 생리적 식염수를 투여하고 방사선조사군은 생리적 식염수를 투여하면서 5Gy를 조사하였다. 홍삼추출물 투여군은 7일 전부터 홍삼추출물(200mg/kg/day)을 투여하면서 5Gy를 조사하였다. Paraquat투여군은 7일 전부터 홍삼추출물(200mg/kg/day)을 투여하면서 paraquat(30mg/kg/day)를 투여하였다. 그리고 각각의 실험군에서 간조직의 H2O2, catalase, MDA를 측정하였다. 그 결과 방사선조사군과 paraquat투여군보다 홍삼추출물 투여군에서 catalase함량이 유의성 있게 증가하여 간의 보호효과가 있었으며 H2O2와 MDA함량도 유의성 있게 감소하였다. 홍삼추출물은 간 조직에 대한 방사선조사 및 paraquat투여로부터 매우 우수한 방호제라고 할 수가 있다.

The protective effects of Red Ginseng on liver damage induced by linac X-ray and paraquat were investigated. To one group of ICR male mice were given in Red Ginseng(200mg/kg/day for 7days, orally) before 5Gy(1.01Gy/min) dose of linac X-ray irradiation. To another group were given in Red Ginseng (200mg/kg/day for 7days, orally) before paraquat(30mg/kg/day, orally) was Radiation irradiation group were given with saline(0.1ml) and 5Gy. Contrast group were given with saline(0.1ml). The levels of H2O2, catalase and MDA in liver tissue were measured. In Red Ginseng to paraquat(RG+PQ) group and Red Ginseng(RG+Rad) group than irradiation group(Rad), the catalase level were significantly increased, and the catalase levels were appeared at radiation protection. The Red Ginseng was significantly decreased to MDA and H2O2 level to paraquat(RG+PQ) group and Red Ginseng(RG+Rad) group than irradiation group(Rad). Therefore, Red Ginseng was very excellent protector on radiation and paraquat of liver in mice.

키워드

참고문헌

  1. Brunori, M and Rotillo, G. "Biochemistry of oxygen radical species" Methods in Enzymol, Vol. 105, pp. 22-35, 1984. https://doi.org/10.1016/S0076-6879(84)05005-9
  2. Misra, H. P and Fridovich, I. "The generation of superoxide radical during the autoxidation of hemoglobin. " J. Biol. Chem, Vol 247, No. 1, pp. 6960-6962. 1972.
  3. Bannister, J. V and Rotilio, G. "Aspects of the structure, function, and applications of superoxide dismutase." CRC, Vol. 22, pp. 111-180, 1987.
  4. Oura, H. Hiai, S. Nakashima, S and Taukada, K., Cham, Pharm. Bull., Vol. 19, pp. 453, 1971. https://doi.org/10.1248/cpb.19.453
  5. Yonezawa, M., N. Katoh, A. Takeda, " Reatoration of Radiation injury by ginseng II. some properties of the Radioprotective substance." J. of radiation research, Vol. 22, No. 3, pp. 336-343. 1981. https://doi.org/10.1269/jrr.22.336
  6. Brawn, K. and Fridovich., I., "DNA strand-scission by enzymatically generated oxygen radicals." Arch. Biochem. Biophys., Vol. 206, pp. 414-419, 1981. https://doi.org/10.1016/0003-9861(81)90108-9
  7. Takeda, A., Yonezawa, M and Katoh, N. "Restoration of radiation injury by ginseng extract." Proceed. of 4th Int. Ginseng Symp. Seoul, pp. 17-20, 1981.
  8. Fridovich, I., "Superoxide dismutases. In : Method of Enzymatic Analysis." H.U. Bergmyer, ed. Academic Verlag. Berilin. Vol. 58, pp 61-97, 1986.
  9. Wolff, S, P., "Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hydro-peroxides." Methods in Enzymology, Vol. 233, pp. 182, 1994. https://doi.org/10.1016/S0076-6879(94)33021-2
  10. Aebi, H. E., "CAT. Insight : Method of enzymatic An analysis." H. U. Bergmyer, ed. Third edition. Verlag. Chemi. Weinheim, Vol. 3, pp. 273, 1982.
  11. Park, Y. S.,. Kim, Y. G., Chang, C. C. and Kim, D. Y., "The effect of Red ginseng extract on Antioxidants and Lipid peroxidation of Liver in r-irradiated mice." Kor. Biochem. J., Vol. 26, No. 2, pp. 184. 1993.
  12. Yokozawa, T., Liu, Z. W. and Dong, E., "A study of ginsenoside-Rd in a renal ischemia-reperfusion model." Nephron Basel., Vol. 78, pp. 201, 1998. https://doi.org/10.1159/000044911
  13. Lee, J. W., "Effect of ethanol administration on glutathione and lipid peroxide levels in rat liver and cerebellum." J. Kor. Sco. Food Nur. Vol. 20, pp. 285, 1991.
  14. Yamaoka, K, Edamatsu, R. and Mori, A., "Increased SOD activities and decreased lipid peroxide levels induced by low dose X-irradiation in rat organs." Free, radic, Biol, Med, Vol. 11, pp. 299, 1991. https://doi.org/10.1016/0891-5849(91)90127-O
  15. Oh, M, H., Chung, H., Yong, H. S., Kim, K. W., Oura, H. and Yokozawa, T., "Effects of ginsenoside-Rb2 on the antioxidants in SAM-R/1 mice." Kor. Biochem. J., Vol.25, pp. 492, 1992.
  16. Kim., M. L. and Choi, K. H., "Inhibitory action of the paraquat on superoxide dismutase of Escherichia coli." J. Korean Soc. Food Nutr., Vol. 23, pp. 849, 1994.
  17. Kum SooSung, Chul Chun, Young Hun Kwon, Kyon Hyun Kim, Che Chul Chang, "Effects of Red Ginseng Component on the Antioxidative Enzymes Activities and Lipid Peroxidation in the Liver of Mice" Journal of Ginseng Research, Vol. 24, pp. 29, 2000.