• Journal of Life Science
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• v.26 no.5
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• pp.614-619
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• 2016

The prostate-apoptosis-response-gene-4 (Par-4) protein has been identified as an effector of cell death in response to various apoptotic stimuli in prostate cancer cells. We found that overexpression of Par-4 by stable transfection inhibits cell migration and invasion in Caki cells. The expression of various matrix metalloproteinases (MMPs) has been implicated in the invasion and metastasis of cancer cells. In this study, we investigated whether ectopic expression of Par-4 modulates MMP-2 expression and activity in human renal carcinoma Caki cells. We found that overexpression of Par-4 markedly inhibited MMP-2 activity, but not MMP-9 activity. However, loss of the leucine zipper domain of Par-4 (Par-4 ΔLZ#1 and #2) did not inhibit MMP-2 activity. Further, knock-down of Par-4 with the corresponding siRNA resulted in increased invasion and metastasis of renal carcinoma Caki cells. Interestingly, overexpression or knock-down of Par-4 did not affect the expression levels of MMP-2 mRNA. Taken together, our findings suggest that Par-4 may inhibit MMP-2 activity through its post-transcriptional regulation in renal carcinoma Caki cells.

• Korean Journal of Veterinary Service
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• v.40 no.1
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• pp.35-40
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• 2017

In order to find a way to reduce histamine formation when storing salted mackerel at home, a series of experiments were carried to monitor the time-related changes of histamine content, total aerobic bacteria and coliform quality of mackerel marketed in Incheon by the type of storage containers (clean, zipper and vacuum bag) during storage at $4^{\circ}C$, $24^{\circ}C$ and $-20^{\circ}C$ for 10 days. Histamine formation was continuously increased with passing time during storage at $4^{\circ}C$, whereas it was decreased after 3 days during storage at $24^{\circ}C$. The initial value of histamine was maintained during storage at $-20^{\circ}C$ for 10 days. During storage at $4^{\circ}C$, total aerobic bacteria and coliform showed a tendency to increased rapidly till 3day and then decreased gradually. The formation of histamine was increased in the order of vacuum bag$4^{\circ}C$.

• Journal of the Korean Chemical Society
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• v.39 no.10
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• pp.776-782
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• 1995

A theory of the helix/coil transition for $\alpha$ helical dimer such as $\alpha$ tropomycin and paramycin is developed. The treatment differs from those formulated previously for oligopeptide dimer which is explained by the matrix method using Zimm-Bragg parameter: In the present treatement, it is explained by the zipper model which can account for the dangling H-bond. We calculate the fractional helicity in $\alpha$ helical dimer as a function of helix initiation $constant(\sigma)$, helix stability constant(${\xi}$) and hydrophobic interaction parameter(w). For $\alpha$ tropomycin, the helix stability profile is also calculated. The transitions of this oligomer due to the change of temperature and the concentration of oligopeptide involve simultaneous dissociation of the dimer. The transitions of dimers which have cross-linked S-S bonds or have long chains don't occur, because they keep always helical structures. The transitons due to the concentration of the oligopeptides are steeper than those due to the chain length or temperature.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.9
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• pp.129-138
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• 2014

Various interpolations for digital imaging devices with a single image sensor have proposed. However, conventional methods did not consider the resolution modus of image sensor using periodic sampling. Therefore, the resulting images have problems such as quality degradation and color artifacts(color moire, zipper). In this paper, we propose a color interpolation algorithm for pixel resolution modus of image sensor. The proposed algorithm consisted of an initial step to compensate edge prediction effectively and refinement step using minimum directions for pixel resolution modus. To analyze a result of the proposed algorithm with conventional methods, we evaluated subjectively using images quality comparison and objectively using PSNR(Peak Signal to Noise Ratio). Experimental results showed that the proposed algorithm was more successful in eliminating the color artifacts than conventional methods judged by both objective and subjective criteria.

• IMMUNE NETWORK
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• v.10 no.2
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• pp.35-45
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• 2010

Background: Expression of recombinant antibodies and their derivatives fused with other functional molecules such as alkaline phosphatase in Escherichia coli is important in the development of molecular diagnostic reagents for biomedical research. Methods: We investigated the possibility of applying a well-known Fos-Jun zipper to dimerize $V_H$ and $V_L$ fragments originated from the Fab clone (SP 112) that recognizes pyruvate dehydrogenase complex-E2 (PDC-E2), and demonstrated that the functional zipFv-112 and its alkaline phosphatase fusion molecules (zipFv-AP) can be produced in the cytoplasm of Origami(DE3) trxB gor mutant E. coli strain. Results: The zipFv-AP fusion molecules exhibited higher antigen-binding signals than the zipFv up to a 10-fold under the same experimental conditions. However, conformation of the zipFv-AP seemed to be influenced by the location of an AP domain at the C-terminus of $V_H$ or $V_L$ domain [zipFv-112(H-AP) or zipFv-112(L-AP)], and inclusion of an AraC DNA binding domain at the C-terminus of VH of the zipFv-112(L-AP), termed zipFv-112(H-AD/L-AP), was also beneficial. Cytoplasmic co-expression of disulfide-binding isomerase C (DsbC) helped proper folding of the zipFv-112(H-AD/L-AP) but not significantly. Conclusion: We believe that our zipFv constructs may serve as an excellent antibody format bi-functional antibody fragments that can be produced stably in the cytoplasm of E. coli.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.160-160
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• 2017

Drought stress is a major constraint of crop development and productivity. Plants have evolutionally developed several mechanisms at the molecular, cellular, and physiological levels to overcome drought stress. The basic Leucine zipper (bZIP) transcription factor (TF) family members are starting to be concerned about their roles in drought stress responses. In this study, we functionally characterized OsbZIP66, a rice group-E bZIP TF, to be associated with rice drought tolerance mechanisms. Expression of OsbZIP66 was significantly induced upon treatments of rice plants with drought, high salinity, and ABA. These observations and the fact that the OsbZIP66 promoter contains ten ABA-responsive elements suggest that OsbZIP66 is up-regulated by drought stress in an ABA-dependent manner. Overexpression of both OsbZIP66 in a whole plant body and specifically in roots enhanced drought tolerance of rice plants, indicating that the rice drought tolerance positively correlates with the expression levels of OsbZIP66. Thus, our results demonstrated that OsbZIP66 has a potential for use in biotechnological development of high-yielding rice plants under drought conditions.

• Fashion & Textile Research Journal
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• v.8 no.2
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• pp.203-208
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• 2006

The purpose of this study is to examine the ergonomic working uniform. Developing a prototype uniform will be the first stage in aiming to create a working uniform in the construction field. This study was conducted using a questionnaire in the construction field of Daegu city, and the data was gathered from 121 questionnaires. Some of the questions that were asked, were about the case of putting on and removing the uniform and if any areas of uniform caused discomfort. The areas of the uniform that caused problems were the waist, knee, shoulder, arm and neck regions. The free range of movement of these regions were restricted and caused the person to feel cramped. The results of the questionnaire called for improvements to the knee, waist, shoulder and crotch area. The prototype that was developed offered more range of movement in the knee and crotch area, while not causing the uniform to be cramped too much during motion. The prototype also improved comfort by raising the waist line of back part, and by having the ability of the uniform to be tightened or loosened with zipper according to the persons preference. The upper area of the uniform was improved by dropping the shoulder seam line which enhanced comfort and also allowed a better fit for the elbow and wrist. The prototypes additional feature included a slit zipper in the sides of the trousers and a gusset in the under arm area to improve breathability and sweat elimination.

• The Korean Journal of Physiology and Pharmacology
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• v.26 no.6
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• pp.457-468
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• 2022

It has been demonstrated that APPL1 (adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1) is involved in the regulation of several growth-related signaling pathways and thus closely associated with the development and progression of some cancers. Diallyl trisulfide (DAT), a garlic-derived bioactive compound, exerts selective cytotoxicity to various human cancer cells through interfering with pro-survival signaling pathways. However, whether and how DAT affects survival of human hepatocellular carcinoma (HCC) cells remain unclear. Herein, we tested the hypothesis of the involvement of APPL1 in DAT-induced cytotoxicity in HCC HepG2 cells. We found that Lys 63 (K63)-linked polyubiquitination of APPL1 was significantly decreased whereas phosphorylation of APPL1 at serine residues remained unchanged in DAT-treated HepG2 cells. Compared with wild-type APPL1, overexpression of APPL1 K63R mutant dramatically increased cell apoptosis and mitigated cell survival, along with a reduction of phosphorylation of STAT3, Akt, and Erk1/2. In addition, DAT administration markedly reduced protein levels of intracellular TNF receptor-associated factor 6 (TRAF6). Genetic inhibition of TRAF6 decreased K63-linked polyubiquitination of APPL1. Moreover, the cytotoxicity impacts of DAT on HepG2 cells were greatly attenuated by overexpression of wild-type APPL1. Taken together, these results suggest that APPL1 polyubiquitination probably mediates the inhibitory effects of DAT on survival of HepG2 cells by modulating STAT3, Akt, and Erk1/2 pathways.

• Journal of Food Hygiene and Safety
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• v.38 no.2
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• pp.63-68
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• 2023

In this study, we analyzed the effect of storage conditions on the survival of fungi in red pepper powder. Red pepper powder was inoculated with a total of six fungal species, namely Aspergillus terreus, Aspergillus flavus, Rhizopus microsporus, Aspergillus fumigatus, Aspergillus niger, and Aspergillus ochraceus at a final cell count of 4-6 log CFU/g. After inoculating the sterilized red pepper powder with fungi, we dried the powder on a clean bench and packaged it in zipper bags. Following drying, the water activity was 0.502±0.001. Subsequently, the red pepper powder inoculated with fungi was stored at -20℃, 5℃, 15℃, and 25℃. All six species of fungi perished the quickest at 25℃ and survived for the longest (168 days) at -20℃. In summary, this study showed that fungi survive for an extended period in red pepper powder at -20℃ and 5℃ compared to 15℃ and 25℃. Therefore, to prevent fungal contamination, red pepper powder should have a water activity below 0.6 and be stored in a zipper bag at room temperature.

• Fashion & Textile Research Journal
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• v.25 no.3
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• pp.324-332
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• 2023

Consumers are addressing the issue of dimensional dissatisfaction by repairing clothes, experimenting with style changes, and looking for cost-effective solutions that result in better-fitting garments. This study investigated the repair status by type of clothing, analyzed the method and frequency of repair by type of clothing category and season, and analyzed consumer redesign activities. The findings revealed that upper garments, such as T-shirts, jumpers, jackets, dress shirts, and dresses, were frequently repaired. The common modifications to upper garments included(in order of frequency) shortening sleeve length, shortening overall length, reducing garment width, zipper repair, and adjusting sleeve width. Lower garments, such as pants, jeans, skirts, and training pants, followed in terms of repair frequency. The modifications to lower garments included(in order of frequency) shortening length, reducing width, adjusting waist width (both narrowing and widening), replacing elastic bands, zipper repair, and lengthening. Repairs were more frequently conducted in the order of autumn, winter, spring, and summer. Repair methods varied depending on the clothing type and alterations involved to the length and width of garments and the replacement or removal of old sections. Redesigning clothing as a recycling method was found to enhance the cost-effectiveness of the collection. The study further confirmed the sustainability aspect of redesigning and reusing clothing.