• Polymer(Korea)
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• v.29 no.3
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• pp.260-265
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• 2005

The initial burst of drug release is an important role in the controlled delivery of drug having hish toxicity and narrow therapeutic ranges. Nanosphere composed of monolayer could not achieve precisely controlled drug release because of the initial burst of drug on surface. In this study, double layered nanosphere was prepared for sustained drug delivery without initial burst. Double layered nanosphere composed of dextran and PLGA was fabricated by using conventional W/O/W double emulsion method. To control surface tension on the outer layer of nanospheres, PVA was used as a surfactant. Release behavior of dextran as model drug was observed as the $3{\times}1$mm wafers formed by compression mould in the deionized water for 7 days. Double layered nanosphere has sustained release behavior, in contast to single layered nanospheres. such as mechanical mixture and dextran nanospheres. Especially, nanosphere containing PVA $0.2\%$ has shown nearly the zero-order release profile. As a result of this study, double layered nanospheres has more sustained release profile of drug without the initial burst and the release behavior of dexoan on tile double layered nanospheres was controlled by the contents of PVA as a surfactant.

• Journal of Microbiology and Biotechnology
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• v.18 no.9
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• pp.1606-1612
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• 2008

Interleukin (IL)-32 is a recently identified proinflammatory cytokine that is one of the IL-18 inducible genes, and plays an important role in autoimmune and inflammatory diseases. We produced antibodies against IL-32 and studied the expression of IL-32 in human stomach cancer. We detected IL-32 secreted from K-562 cells which were stably transfected with IL-32 and in the sera of stomach cancer patients by a sandwich ELISA using a monoclonal antibody KU32-52 and a polyclonal antibody. In order to optimize a sandwich immunoassay, recombinant IL-32a was added, followed by the addition of a biotinylated KU32-52 into microtiter plate wells precoated with a goat anti-IL-32 antibody. The bound biotinylated KU32-52 was probed with a streptavidin conjugated to HRP. This sandwich ELISA was highly specific and had a minimal detection limit of 80 pg/ml (mean${\pm}$SD of zero calibrator) and measuring up to 3,000 pg/ml. This ELISA showed no cross-reaction with other cytokines such as hIL-1$\alpha$, hIL-1$\beta$, hIL-2, hIL-6, hIL-8, hIL-10, hIL-18, and hTNF-$\alpha$. Intra-assay coefficients of variation were 18.5% to 4.6% (n=10), and inter-assay coefficients were 23% to 9% (n=10). The average IL-32 level in the sera of 16 stomach cancer patients (189 pg/ml) was higher than that of 12 healthy control men (109 pg/ml). Our results indicate that serum IL-32 level can be detected by using an established ELISA, and that this immunoassay and mAb KU32-09 specific for immunohistochemistry can be used in the detection of expressed and secreted IL-32 in stomach cancer patients.

• Resources Recycling
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• v.19 no.4
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• pp.58-64
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• 2010

In order to select the proper collector for the flotation of malachite, we had floated malachite by using various collectors. When measuring the zeta potential of malachite, we knew that the point of zero charge of malachite is found at pH 8.2 and malachite particles are negatively charged above this pH value; and positively, below this pH value. The floatability of malachite by the anionic collectors like sodium oleate and Aeropromoter 845, was about 97% in pH 5~11 range, whereas that of malachite by alkyl hydroxamate and the cationic collector like dodecyl trimethyl ammonium chloride and dodecyl ethyl methyl ammonium chloride, was below 15% above pH 5 regardless of dosage increase of collector and flotation time. The malachite sulphidised by $(NH_4)_2S$ was floated above 97% by potassium amyl xanthate. The dosage of potassium amyl xanthate decreased and the flotation period shortened to one third than when it was not sulphidised by $(NH_4)_2S$. Therefore, potassium amyl xanthate and the anionic collectors like sodium oleate and Aeropromoter 845 can be used as the collector of malachite.

• Journal of the Korean earth science society
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• v.28 no.7
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• pp.936-946
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• 2007

Analytical geopotential field in balance with the sectoral mode (the first symmetric mode with respect to the equator) of the Rossby-Haurwitz wave on the inclined rotation axis was derived in presence of superrotation background flow. The balanced field was obtained by inverting the divergence equation with the time derivative being zero. The inversion consists of two steps, i.e., the evaluation of nonlinear forcing terms and the finding of analytical solutions based on the Poisson's equation. In the second step, the forcing terms in the from of Legendre function were readily inverted due to the fact that Legendre function is the eigenfunction of the spherical Laplacian operator, while other terms were solved either by introducing a trial function or by integrating the Legendre equation. The balanced field was found to be expressed with six zonal wavenumber components, and shown to be of asymmetric structure about the equator. In association with asymmetricity, the advantageous point of the balanced field as a validation method for the numerical model was addressed. In special cases where the strength of the background flow is a half of or exactly the same as the rotation rate of the Earth it was revealed that one of the zonal wavenumber components vanishes. The analytical balanced field was compared with the geopotential field which was obtained using a spherical harmonics spectral model. It was found that the normalized difference lied in the order of machine rounding, indicating the reliability of the analytical results. The stability of the sectoral mode of Rossby-Haurwitz wave and the associated balanced field was discussed, comparing with the flrst antisymmetric mode.

• Applied Biological Chemistry
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• v.44 no.3
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• pp.137-142
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• 2001

In order to prove physiological function of biopolymer from Bacillus coagulans CE-74, in vitro experiments simulating the passive membrane transport of gastrointestinal tract were carried out using dialysis membrane. And inhibition effect of isolated biopolymer on tyrosinase and angiotensin converting enzyme (ACE) were observed. The glucose retardation index after 30 min dialysis was 43.5% in the presence of 2% biopolymer. As the dialysis period became longer, the retarding effect toward glucose absorption decreased and the effect was close to zero after 5 hr dialysis. The bile acid retardation index after 30 min dialysis was 34% and 44.2% in the presence of 1% and 2% biopolymer, respectively. The effect decreased as the dialysis time elapsed. It was measured by arosinase inhibition activity of biopolymer that inhibition effect was 48.5% in $20\;{\mu}g/{\mu}l$. In a ACE inhibition activity, biopolymer showed inhibition activity as 97% in $10\;{\mu}g/{\mu}l$.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.6
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• pp.628-636
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• 2008

Anaerobic denitrification in a two phase anaerobic digestion(TPAD) process combined with biological nutrients removal (BNR) system was studied for a piggery wastewater treatment. Denitrification efficiency and the effects of the nitrified effluent on acidification was investigated by recycling the nitrified effluent to the acidogenic reactor. Recycle of the nitrified effluent to the acidogenic reactor enhanced the conversion efficiency of the influent COD into volatile fatty acids(VFAs) in the TPAD-BNR system treating the piggery wastewater. Acidification rate of the acidogenic sludge acclimated with the nitrified effluent showed 6 times higher than that acclimated without it. VFA could be used for denitrification as carbon sources, however, nitrate could enhance acidification activity in the acidogenic reactor. VFA production rate was affected on the COD/Nitrate(COD/N) ratio, however, it depended much more whether the acidogenic sludge acclimated with nitrate or not. Denitrification with the acidogenic sludge acclimated without nitrified effluent followed zero-order reaction and the reaction rate constants were in the range of 1.31$\sim$1.90 mg/L$\cdot$h. Denitrification reaction rate constants of the acidogenic sludge acclimated with nitrified effluent were 3.30 mg/L$\cdot$h that showed almost twice of them evaluated from the previous tests. The stoichiometric ratios of utilized COD to removed nitrate showed similar in both tests which were in the range of 5.1$\sim$6.4 at COD/N ratio of 10.

• Journal of KIISE:Software and Applications
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• v.36 no.2
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• pp.131-143
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• 2009

Based on the fact that a human being walks naturally and stably with consuming a minimum energy, this paper proposes a new method of generating a natural gait of 5-link biped robot like human by analyzing a COG (Center Of Gravity) trajectory of human's gait. In order to generate a natural gait pattern for 5-link biped robot, it considers the COG trajectory measured from human's gait images on the sagittal and frontal plane. Although the human and 5-link biped robot are similar in the side of the kinematical structure, numbers of their DOFs(Degree Of Freedom) are different. Therefore, torques of the human's joints cannot are applied to robot's ones directly. In this paper, the proposed method generates the gait pattern of the 5-link biped robot from the GA algorithm which utilize human's ZMP trajectory and torques of all joints. Since the gait pattern of the 5-link biped robot model is generated from human's ones, the proposed method creates the natural gait pattern of the biped robot that minimizes an energy consumption like human. In the side of visuality and energy efficiency, the superiority of the proposed method have been improved by comparative experiments with a general method that uses a inverse kinematics.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.10
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• pp.2619-2627
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• 2009

The ground fault is occupying 70% among the total number of faults in ungrounded distribution power system. When the ground fault occurs in ungrounded system, the fault current is so small that it is hard to detect. But fault handling is very important because to continue power supply during fault conditions may cause the fault spreading and the distribution device in trouble. This paper presents the fault line detection method by using GPT signal detecting zero sequence voltage, and the fault section detection method by detecting whether GPT signal is disappeared or not during shifting normally open switch, which is connecting switch between distribution lines with open state in order to restore the outage area under emergency situation, and during isolating each section one by one which belongs to the fault line. This method is efficient because there is no whole power interruption during the fault section detection, and it is possible to perform both the fault section detection and the service restoration for the outage area at the same time, and it can apply to various distribution system configuration. Program for the fault restoration was developed applying proposed method, and it has been validated by applying to the pilot project of distribution automation system in Vietnam which has the ungrounded distribution system.

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.1
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• pp.101-111
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• 2018

The Korean government has been trying to conserve a marine ecosystem that has been shifting due to climate change. As part of this effort, the government designated seventy-seven marine species that have been disappearing and deserve to be protected as endangered managing them specially. To generate basic data to guide policy for these endangered species, their value must be measured. OOf the species declared endangered, coral is particularly threatened by climate change, and its management is important. Accordingly, understanding the potential value of reefs, can be an effective way of proving the benefits of continuous management to decision makers and the general public alike. To this end, we have applied the contingent valuation method (CVM), an economic technique of for valuing a environmental and non-market goods such as a coral reef. A national face-to-face survey of 1,000 randomly selected households was conducted in order to determine the public's willingness to pay (WTP) for conserving coral reefs. A one-and-one-half-bound (OOHB) model was adopted to interpret WTP responses, and a spike model was employed to deal with zero WTP responses. The results show that the conservation value of a coral reef can be estimated at 3,016 won per household per year, statistically significant at the 1 % level. Expanding values to the national population gives an annual value of 58.9 billion won. We can conclude that the public is willing to pay a significant amount to conserve coral reefs.

• Journal of Korean Society for Atmospheric Environment
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• v.33 no.5
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• pp.480-496
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• 2017

CMAQ (Community Multiscale Air Quality)-HDDM (High-order Direct Decoupled Method) simulations with MICS-Asia 2010 and INTEX-B 2006 emissions inventories were performed to investigate the impact of Chinese $NO_x$ and VOC emissions on 1-hr ozone concentrations over South Korea during May to July in 2014. Chinese $NO_x$ and VOC emissions in MICS-Asia 2010 were 60% higher and 100% lower than those in INTEX-B 2006 during the simulation period. It makes the ratio of Chinese VOC to $NO_x$ emissions in INTEX-B 2006 (Case 1) is 3.2 times higher than that in MICS-Asia 2010 (Case 2). When the observed period mean 1-hr ozone concentration averaged across 106 air monitoring sites in the SMA (Seoul Metropolitan Area) was 37.6 ppb, the modeled values were similar to each other; 37.3 ppb for Case 1, and 40.4 ppb for Case 2. Both cases show that daily maximum 1-hr ZOC (Zero-Out Contribution) of Chinese $NO_x$ and VOC emissions were as high as 55 ppb and 35 ppb for the episode respectively. Correlation coefficients between ZOC of Chinese $NO_x$ and VOC emissions and the SMA daily maximum 1-hr ozone were 0.49~0.69. It indicates that Chinese emissions occasionally affect the SMA daily ozone peaks. On the other hand, Case 2 predicted 7 ppb and 1 ppb higher ZOC of Chinese $NO_x$ and VOC emissions than Case 1, when simulated ozone in the SMA is over 80 ppb. It implies that upwind $NO_x$ emissions would be more important than upwind VOC emissions for the long-range transport of ozone in Northeast Asia.