• 한국식품과학회지
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• 제29권6호
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• pp.1184-1190
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• 1997

Corn zein을 코팅한 carrageenan복합필름을 미리 제조한 carrageenan필름을 polyethylene glycol (20% (w/w) of CZ)과 glycerol (24% (w/w) of CZ)을 함유하는 corn zein 용액(10, 20 and 30% in 95% ethyl alcohol)에 침지하여 제조하였다. 각 필름에 대하여 수증기투과도, 수분용해도, 팽윤도, 인장강도, 연신도, 열접착강도 및 색도를 측정하였다. carrageenan필름의 수증기 투과도는 corn zein 용액의 농도가 증가할수록 유의적인 차이(p<0.05)를 나타내며 감소하였다. 필름의 수분 용해도와 팽윤도는 corn zein 용액의 농도가 증가할수록 직선적으로 감소하였으며, 인장강도 역시 코팅용액의 농도에 대하여 직선적인 감소를 나타냈다. 코팅용액의 농도에 관계없이 모든 코팅필름은 열접착특성을 갖는것으로 나타내었으며, 이들의 열접착강도는 corn zein 필름의 약 절반에 미달하는 수준이었다. corn zein으로 코팅을 함으로 필름의 색도의 변화를 나타냈는데 이는 황색도(Hunter b-value)의 증가에 기인하였다.

• 한국식품조리과학회지
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• 제29권6호
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• pp.647-652
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• 2013

Solubility profiles of zein and carotenoid in aqueous ethanol were studied. Zein showed minimum turbidity at the aqueous ethanol concentration of 87-92%, indicating least aggregations between protein molecules. Solubilities of zein and carotenoid increased linearly with the content of yellow zein up to 20% in the aqueous ethanol range of 60-95% tested. At room temperature of $20^{\circ}C$, zein showed maximum solubility in broad ethanol concentration ranges of 60-95%, while that for carotenoid was somewhat narrower with ethanol concentration range of 85-95%. However, at incubation temperature of $-20^{\circ}C$, solubilities of both carotenoid and zein were lowered, with dramatic reduction being exhibited at aqueous ethanol concentration of 60% for both compounds, while substantial reduction in solubility was shown at 95% ethanol by zein only. Zein was practically insoluble in absolute ethanol, regardless of temperature range tested, while carotenoid remained largely soluble, though there was pronounced decrease in solubility at the subfreezing temperature.

• Preventive Nutrition and Food Science
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• 제8권4호
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• pp.343-348
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• 2003

To elucidate the effect of gamma-irradiation on the physicochemical properties of zein films, the molecular and mechanical properties of the films were examined after irradiation at various irradiation doses. Gamma-irradiation of zein solutions caused the disruption of the ordered structure of the zein molecules, as well as degradation, cross-linking, and aggregation of the polypeptide chains based on an SDS-PAGE study. Gamma-irradiation increased the solubility of zein and decreased the viscosity due to cleavage of the polypeptide chains. Protein solubility of the zein films in urea/2-mercaptoethanol also increased with increasing irradiation doses. Alterations of the zein molecules by irradiation decreased water vapor per-meability by 12% and increased the elongation of zein films. However, mean tensile strength of the zein films was decreased by gamma-irradiation treatment. Measurement of Hunter color values indicated that irradiation caused a destructive effect on yellow pigments, resulting in a significant decrease in Hunter b values. The microstructure as observed by scanning electron microscopy showed that irradiated zein film had a smoother and glossier surface than the non-irradiated films.

• 한국염색가공학회지
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• 제23권4호
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• pp.227-232
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• 2011

Zein is a hydrophobic protein produced from maize and has great potential in a number of industrial applications, such as food, food coating and food packaging. To obtain suitable electrospinning conditions for thinner and uniform zein nanofiber mats, a series of experiments was conducted on various volume ratios (v/v) of ethanol/water solutions with different zein concentrations. The prepared zein nanofiber mats were characterized by field emission scanning electron microscopy and contact angle measurements. Uniform zein fibers with a average diameter in the nanometer scale (300~500 nm) could be prepared from 30 wt.% zein in 7/3 (v/v) ethanol/water solutions.

• KSBB Journal
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• 제27권4호
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• pp.237-242
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• 2012

In this study, zein microparticles and drug-loaded zein microparticles were prepared using supercritical ASES technique. The effects of operating parameters on particle size and morphology were investigated. ASES-processed zein microparticles consisted of agglomerates of very fine unit particles. As temperature increased, the size of unit particles increased and their morphology became more spherical. The addition of water to the solvents for zein resulted in the formation of more spherical microparticles. The release characteristics of drug-loaded zein microparticles were also studied.

• Current Research on Agriculture and Life Sciences
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• 제31권1호
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• pp.11-17
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• 2013

To investigate the effects of co-solvents on the morphology of nano-scale zein materials, zein solutions were electrospun with different co-solvent ratios of EtOH/$H_2O$. Different zein solution concentrations were used to study the effects of the zein content on the electrospun materials. The resulting electrospun materials were all characterized using field-emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM). The diameters of the electrospun nanoparticles and nanofibers were found to increase when increasing the EtOH ratio at certain zein concentrations. Furthermore, increasing the zein content changed the morphology of the electrospun materials from nanoparticles to nanofibers.

• 한국식품과학회지
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• 제26권2호
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• pp.138-140
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• 1994

Zein과 gluten을 이용하여 시험제조한 분해성 포장지의 기능성을 조사하였다. Zein과 gluten 포장지의 인장강도는 각각 $30.1{\sim}138.5\;MPa$, $37.1{\sim}98.6\;MPa$ 범위를 나타냈으며 제조된 포장지의 두께가 두꺼워질수록 인장강도는 증가하였다. 용해도는 zein 포장지가 1.3%로서 gluten 포장지 및 셀룰로오스 포장지의 용해도에 비하여 상대적으로 낮게 나타났다. 한편, 수증기투과도는 zein 포장지의 경우 $0.0711{\sim}0.01778\;g\;mm/m^2\;hr\;mmHg$, gluten 포장지의 경우 $0.2134{\sim}0.2972\;g\;mm/m^{2}\;hr\;mmHg$였으며 인장강도와 마찬가지로 제조된 포장지의 두께가 두꺼워질수록 증가하였다.

• Journal of Microbiology and Biotechnology
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• 제17권3호
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• pp.520-523
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• 2007

Edible films of gelatin and com zein were prepared by incorporating nisin to the film-forming solutions. Com zein film with nisin of 12,000 IU/ml had an increase of 11.6 MPa in tensile strength compared with the control, whereas gelatin film had a slight increase with the increase of nisin concentration added. Water vapor permeability for both com zein and gelatin films decreased with the increase of nisin concentration, thus providing a better barrier against water. Antimicrobial activity against Listeria monocytogenes increased with the increase of nisin concentration, resulting in 1.4 log cycle reduction for com zein film and 0.6 log cycle reduction for gelatin film at 12,000 IU/ml. These results suggest that incorporation of nisin into com zein and gelatin films improve the physical properties of the films as well as antimicrobial activity against pathogenic bacteria during storage, resulting in extension of the shelf life of food products by providing with antimicrobial edible packaging films.

• 한국고분자학회:학술대회논문집
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• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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• pp.360-360
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• 2006

Zein protein is one of the best biopolymer for edible film making and polyols are convenient plasticizers for biopolymers. Sorbitol, glycerol and manitol at three levels (0.5, 0.7, 1g/g of zein) were used as plasticizers. Rheological and thermal properties of zein resins were studied for determining their plasticization effectiveness. Sorbitol and glycerol had good plasticizing effects and could decrease viscoelastic modulus of zein resins considerably, but manitol was not as effective as them. Effects of plasticizers on thermal properties of resins were investigated by DSC at -100 to $150^{\circ}C$. No crystallization and melting peaks related to zein resin and plasticizers were observed. Thermograms showed that polyolic plasticizers and zein resin remained a homogeneous material throughout the cooling and heating cycles.

• BMB Reports
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• 제31권2호
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• pp.196-200
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• 1998

Zeins, maize storage proteins, are retained in the endoplasmic reticulum (ER) during the subcellular targeting process without the ER retention signal. Circumstantial data indicate that the 27K zein is an ER transmembrane protein. The potential transmembrane domain may permit the 27K zein to remain in the ER. This study investigated the potential transmembrane feature by employing alkaline extraction, proteinase K digestion, and surface biotinylation on isolated intact protein bodies. These assays consistently support the possibility of the 27K zein as a transmembrane protein. The 27K zein polypeptide was shown to be associated with alkali-stripped membranes. The polypeptide was digested by proteinase K to a smaller fragment. According to surface biotinylation, the 27K zeins was labeled to the exclusion of other classes of zeins. This study, therefore, concludes that the 27K zein has an ER transmembrane domain, which may serve as an anchor for zeins' ER retention.