• KSII Transactions on Internet and Information Systems (TIIS)
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• 제17권12호
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• pp.3364-3382
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• 2023

Remote sensing image segmentation plays an important role in realizing intelligent city construction. The current mainstream segmentation networks effectively improve the segmentation effect of remote sensing images by deeply mining the rich texture and semantic features of images. But there are still some problems such as rough results of small target region segmentation and poor edge contour segmentation. To overcome these three challenges, we propose an improved semantic segmentation model, referred to as MRU-Net, which adopts the U-Net architecture as its backbone. Firstly, the convolutional layer is replaced by BasicBlock structure in U-Net network to extract features, then the activation function is replaced to reduce the computational load of model in the network. Secondly, a hybrid multi-scale recognition module is added in the encoder to improve the accuracy of image segmentation of small targets and edge parts. Finally, test on Massachusetts Buildings Dataset and WHU Dataset the experimental results show that compared with the original network the ACC, mIoU and F1 value are improved, and the imposed network shows good robustness and portability in different datasets.

• Asian-Australasian Journal of Animal Sciences
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• 제33권12호
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• pp.1948-1956
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• 2020

Objective: The purpose of this study was to reveal the metabolic shift in the fungus cocultured with the methanogen (Methanobrevibacter thaueri). Methods: Gas chromatography-mass spectrometry was used to investigate the metabolites in anaerobic fungal (Pecoramyces sp. F1) cells and the supernatant. Results: A total of 104 and 102 metabolites were detected in the fungal cells and the supernatant, respectively. The partial least squares-discriminant analysis showed that the metabolite profiles in both the fungal cell and the supernatant were distinctly shifted when co-cultured with methanogen. Statistically, 16 and 30 metabolites were significantly (p<0.05) affected in the fungal cell and the supernatant, respectively by the co-cultured methanogen. Metabolic pathway analysis showed that co-culturing with methanogen reduced the production of lactate from pyruvate in the cytosol and increased metabolism in the hydrogenosomes of the anaerobic fungus. Citrate was accumulated in the cytosol of the fungus co-cultured with the methanogen. Conclusion: The co-culture of the anaerobic fungus and the methanogen is a good model for studying the microbial interaction between H₂-producing and H₂-utilizing microorganisms. However, metabolism in hydrogenosome needs to be further studied to gain better insight in the hydrogen transfer among microorganisms.

• Steel and Composite Structures
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• 제33권4호
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• pp.489-507
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• 2019

In order to obtain high bearing capacity and good ductility simultaneously, a structural column with hybrid normal and high strength steel (HNHSS) welded box section has been developed. Residual stress is an important factor that can influence the behaviour of a structural member in steel structures. Accordingly, the magnitudes and distributions of residual stresses in HNHSS welded box sections were investigated experimentally using the sectioning method. In this study, the following four box sections were tested: one normal strength steel (NSS) section, one high strength steel (HSS) section, and two HNHSS sections. Based on the experimental data from previous studies and the test results of this study, the effects of the width-to-thickness ratio of plate, yield strength of plate, and the plate thickness of the residual stresses of welded box sections were investigated in detail. A unified residual stress model for NSS, HSS and HNHSS welded box sections was proposed, and the corresponding simplified prediction equations for the maximum tensile residual stress ratio (${\sigma}_{rt}/f_y$) and average compressive residual stress ratio (${\sigma}_{rc}/f_y$) in the model were quantitatively established. The predicted magnitudes and distributions of residual stresses for four tested sections in this study by using the proposed residual stress model were compared with the experimental results, and the feasibility of this proposed model was shown to be in good agreement.

• 한국미생물·생명공학회지
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• 제47권1호
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• pp.87-95
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• 2019

Esterase produced by Acinetobacter sp. B1 (strain B1) was optimized by means of one-variable-at-a-time and response surface methodologies. Results of the one-variable-at-a-time experiment showed that Tween 80 significantly increased esterase production of strain B1. The addition of Tween 80 to the culture medium increased the biomass and esterase activity of strain B1, stimulated content of total extracellular protein, and enhanced the oleic acid (C18:1) composition in the cell membrane of strain B1. The influence of eight culture variables on esterase production was evaluated by Plackett-Burman design. Results showed that Tween 80, pH, and $K_2HPO_4$ significantly affected the esterase production of strain B1. Tween 80, pH, and $K_2HPO_4$ were further optimized by central composite design. Under the optimized conditions (w/v, soluble starch 2.5%, tryptone 1.5%, Tween 80 0.8%, $K_2HPO_4$ 0.5%, NaCl 0.5%, pH 8.0, inoculum size 1%, and inoculum age 24 h), the maximum esterase activity of strain B1 was 152.13 U/ml, which was 10-fold higher than that of non-optimization after 36 h cultivation.

• Molecules and Cells
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• 제45권5호
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• pp.317-328
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• 2022

Trophoblasts, important functional cells in the placenta, play a critical role in maintaining placental function. The heterogeneity of trophoblasts has been reported, but little is known about the trophoblast subtypes and distinctive functions during preeclampsia (PE). In this study, we aimed to gain insight into the cell type-specific transcriptomic changes by performing unbiased single-cell RNA sequencing (scRNA-seq) of placental tissue samples, including those of patients diagnosed with PE and matched healthy controls. A total of 29,006 cells were identified in 11 cell types, including trophoblasts and immune cells, and the functions of the trophoblast subtypes in the PE group and the control group were also analyzed. As an important trophoblast subtype, extravillous trophoblasts (EVTs) were further divided into 4 subgroups, and their functions were preliminarily analyzed. We found that some biological processes related to pregnancy, hormone secretion and immunity changed in the PE group. We also identified and analyzed the regulatory network of transcription factors (TFs) identified in the EVTs, among which 3 modules were decreased in the PE group. Then, through in vitro cell experiments, we found that in one of the modules, CEBPB and GTF2B may be involved in EVT dysfunction in PE. In conclusion, our study showed the different transcriptional profiles and regulatory modules in trophoblasts between placentas in the control and PE groups at the single-cell level; these changes may be involved in the pathological process of PE, providing a new molecular theoretical basis for preeclamptic trophoblast dysfunction.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2023년도 학술발표회
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• pp.142-142
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• 2023

Humans inevitably and continuously produce wastewater in daily life worldwide. To decrease the degradation of river water bodies and aquatic ecosystem therein, humans have built systems at different scales to collect, drain, and treat household-produced wastewater. Particularly, municipal wastewater treatment plants (WWTPs) with centralized controls have played a key role in reducing loads of nutrients in domestic wastewater for the last few decades. Notwithstanding such contributions, impaired rivers regarding water quality and habitat integrity still exist at the whole river basin scale. It is highly attributable to the absence of dilution capacity of receiving streams and/or the accumulation of the pollutant loads along flow paths. To improve the perspective for individual WWTPs assessment, the first crucial step is to achieve systematic understanding on spatial distribution characteristics of all WWTPs together in a given river basin. By taking the initiative, our former study showed spatial hierarchical distributions of WWTPs in three large urbanized river basins in Germany. In this study, we uncover how municipal WWTPs in the contiguous United States are distributed along river networks in a give river basin. The extended spatial scope allows to deal with wide ranges in geomorphological attributes, hydro-climatic conditions, and socio-economic status. Furthermore, we identify the relation of the findings with multiple factors related to human activities, such as the spatial distribution of human settlements, the degree of economy development, and the fraction of communities served by WWTPs. Generalizable patterns found in this study are expected to contribute to establishing viable management plans for recent water-environmental challenges caused by WWTP-discharges to river water bodies.

• Bulletin of the Korean Chemical Society
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• 제28권10호
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• pp.1656-1660
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• 2007

Using solid inorganic peroxides (including Li2O2, Na2O2, SrO2 and BaO2) as starting materials, three reaction paths for singlet oxygen (1O2) production were developed and studied. Their 1O2 emission spectra in the near- IR region and visible region from these reaction paths were simultaneously recorded by a near-IR sensitive Optical Multichannel Analyzer and a visible sensitive Optical Spectrum Analyzer, respectively. The comparison of their 1O2 emission spectra indicated that: (1) in term of the efficiency for 1O2 production, the gasliquid- solid reaction path (in which Cl2 or HCl and H2O reacted with the solid inorganic peroxides suspension in CCl4) was prior to the gas-solid reaction path (in which Cl2 or HCl reacted with the solid inorganic peroxides suspension in CCl4), but was inferior to the gas-liquid reaction path (in which Cl2 or HCl reacted with the solid inorganic peroxides solution in H2O or D2O); (2) the alkali metal peroxides (such as Li2O2 and Na2O2) was prior to the alkaline earth metal peroxides (such as SrO2 and BaO2) as the solid reactants, and Cl2 was favorable than HCl as the gas reactant in efficiency for 1O2 production in these reaction paths.