• Title/Summary/Keyword: yu-gen

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Japanese Traditional Elements in Tadao Ando's Architecture (안도 타다오의 작품에 표현된 일본 전통적 요소)

  • Kim, Dong-Young
    • Journal of the Korean housing association
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    • v.17 no.6
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    • pp.91-98
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    • 2006
  • The stage for Tadao Ando's architectural works is not only in Japan but extends to many countries around the world. His creations are not limited to the world of architecture, but are widely acclaimed supported by public people as well. Why Tadao Ando's works are acknowledged and acclaimed so extensively? Japanese traditional elements used in the his works makes it so. Tadao Ando argued intuition and insanity which were deprived by modernism could recover with locality and it's historic elements laid importance to the self. To realize that, it is necessary the regeneration of landscape which was based on the site and self-reflection must accomplished with the nature. So as to regeneration of landscape, he used Japanese Landscape-gardening and make the nature condensed. With the su-ki, he made the architectural elements simple. And with the Japanese detoured circulation, he made deep and various space. In that way, he intended the nature which is changed with his idea experienced the way of yu-gen.

Overproduction of Recombinant Human VEGF (Vascular Endothelial Growth Factor) in Chinese Hamster Ovary Cells

  • Lee, Seong-Baek;Park, Jeong-Soo;Lee, Seung-Hee;Park, Jun-Ho;Yu, Sung-Ryul;Kim, Hee-Chan;Kim, Dong-Jun;Byun, Tae-Ho;Baek, Kwang-Hee;Ahn, Young-Joon;Yoon, Jae-Seung
    • Journal of Microbiology and Biotechnology
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    • v.18 no.1
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    • pp.183-187
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    • 2008
  • Vascular endothelial growth factors (VEGFs) are a family of proteins that mediate angiogenesis. $VEGF_{165}$ is a VEGF-A isoform and has been extensively studied owing to its potential use in therapeutic angiogenesis. This study established Chinese hamster ovary (CHO) cells overexpressing recombinant human $VEGF_{165}$ $(rhVEGF_{165})$ protein. The production rate of the established CHO cells was over 80mg/l of $rhVEGF_{165}$ protein from a 7-day batch culture process using a 7.5-l bioreactor with a 5-l working volume and serum-free medium. The $rhVEGF_{165}$ protein was purified to homogeneity from the culture supernatant using a two-step chromatographic procedure that resulted in a 48% recovery rate. The purified $rhVEGF_{165}$ protein was a glycosylated homodimeric protein with a higher molecular weight (MW) than the protein expressed from insect cells, suggesting that the glycosylation of the $rhVEGF_{165}$ protein in CHO cells differed from that in insect cells. The purified $rhVEGF_{165}$ protein in this study was functionally active with a half-maximal effective concentration of 3.8ng/ml and specific activity of $2.5{\times}10^5U/mg$.

Characterization of the performance of the next-generation controller for the BOES CCD

  • Park, Su-Hwan;Yu, Young Sam;Sung, Hyun-Il;Park, Yoon-Ho;Lee, Sang-Min;Bang, Seung-Cheol;Chun, Moo-Young;Seong, Hyeon-Cheol;Kim, Minjin
    • The Bulletin of The Korean Astronomical Society
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    • v.46 no.2
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    • pp.76.2-76.2
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    • 2021
  • We present the characterization of the performance of the next-generation controller (SDSU Gen III) for BOAO Echelle Spectrograph CCD (BOES CCD) at the Bohyunsan Optical Astronomy Observatory. The current controller (SDSU Gen II) of the BOES CCD will be upgraded to SDSU Gen III to provide a more stabilized operation. To assess the performance of the new controller (e.g., conversion gain, full well capacity, S/N), we obtain various types of calibration images (e.g., bias, flat, science images of standard stars). Based on those datasets, we find that the overall performance of the new controller is somewhat comparable to that of the old controller if the slow mode is adopted for the readout. This may demonstrate that the new controller can be successfully substituted for the old controller without a substantial loss of performance. However, further analysis with a large dataset obtained in various observational conditions is necessary to confirm our results.

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Research on the Implementation of Infrared Threat Surrogate for DIRCM Performance Analysis (지향성 적외선 방해장치 성능분석을 위한 위협체모사기 구축에 관한 연구)

  • Chun, Seung-Woo;Kim, Joy;Cho, Soo-Hyung;Kim, Jae-Hyup;Yu, Hyun-Geun
    • Journal of the Korea Society of Computer and Information
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    • v.20 no.3
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    • pp.97-106
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    • 2015
  • In this paper, the development of infrared threat surrogate system is described and it can be used to verify the performance of DIRCM system. In this research, threat surrogates are manufactured using same F# and reflective optics used in 1st- and 2nd-Gen. real seeker so that the threat surrogate system can be used to develop code-based jamming techniques. Also the threat surrogate system can analyze the saturation jamming effect for image seeker using FPA detector. The result shows that 1st- and 2nd-generation threat surrogates have relatively precise tracking performance and jamming effect.

Identification of Differentially Expressed Genes in the Longissimus Dorsi Muscle Tissue between Duroc and Erhualian Pigs by mRNA Differential Display

  • Pan, P.W.;Zhao, S.H.;Yu, M.;Liu, B.;Xiong, T.A.;Li, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.7
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    • pp.1066-1070
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    • 2003
  • In order to identify differentially expressed mRNAs (which represent possible candidates for significant phenotypic variances of muscle growth, meat quality between introduced European and Chinese indigenous pigs) in the longissimus dorsi muscle tissue between adult Duroc and Erhualian pigs, mRNA differential display was performed. Five 3' anchor primers in combination with 20 different 5' arbitrary primers (100 primer sets) were used and nearly 5,000 cDNA bands were examined, among which 10 differential display cDNAs were obtained, cloned and sequenced. Six of the 10 cDNAs showed similarity to identified genes from GenBank and the other 4 had no matches in GenBank. Differential expression was tested by Northern blot hybridization and could be confirmed for 2 cDNAs. The method used in this study provides a useful molecular tool to investigate genetic variation that occurs at the transcriptional level between different breeds.

Idle Slots Skipped Mechanism based Tag Identification Algorithm with Enhanced Collision Detection

  • Su, Jian;Xu, Ruoyu;Yu, ShiMing;Wang, BaoWei;Wang, Jiuru
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.14 no.5
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    • pp.2294-2309
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    • 2020
  • In this article, a new Aloha-based tag identification protocol is presented to improve the reading efficiency of the EPC C1 Gen2-based UHF RFID system. Collision detection (CD) plays a vital role in tag identification process which determines the efficiency of anti-collision protocols since most Aloha-based protocols optimize the incoming frame length based on the collisions in current frame. Existing CD methods are ineffective in identifying collision, resulting in a degradation of identification performance. Our proposed algorithm adopts an enhanced CD (ECD) scheme based on the EPC C1 Gen2 standard to optimize identification performance. The ECD method can realize timely and effective CD by detecting the pulse width of the randomly sent by tags. According to the ECD, the reader detects the slot distribution and predicts tag cardinality in every collision slot. The tags involved in each collision slot are identified by independently assigned sub-frames. A large number of numerical results show that the proposed solution is superior to other existing anti-collision protocols in various performance evaluation metrics.

Automatic Generation of Web-based Database Applications (웹 기반 데이타베이스 응용의 자동 생성)

  • Eum, Du-Heon;Yu, Eun-Ja
    • Journal of KIISE:Databases
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    • v.29 no.3
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    • pp.207-216
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    • 2002
  • Nowadays, we can easily access a remote database as well as a local database with HTML forms. Although implementing a database application with HTML forms is much simpler than implementing it with a proprietary graphical user Interface system, HTML forms and CGI programs still must be coded. We implemented a software tool that automatically generates the SQL statements that create a database for an application, the forms that are used as a user interface, and the Java servlets that retrieve the data requested through the forms. The database tables to be treated and the forms to be generated are determined by the class diagram for the application. Our software tool, which we call WebSiteGen, thus simplifies the implementation of a Web-based database application.