• Research in Plant Disease
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• 제8권2호
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• pp.92-100
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• 2002

For the survey of viruses infected in peanut cultivated in Korea, peanut seeds and leaves showing viral symptoms were collected from their growing areas. Typical symptoms on virus infected peanut leaves including mosaic, mottle with necrosis, yellowing, stripe or vein banding and stunts were observed. Two viruses isolated from the naturally infected peanuts were identified as Bean common mosaic virus(BCMV-PSt) and Peanut mottle virus(PeMoV) by their host range, immunosorbent elcetron microscopy(ISEM), direct immuno staining assay(DISA), RT-PCR, and intracellural symptoms. Direct negative staining method by electron microscope showed filamentous particles of about 780 m in length as well as inclusion bodies. In ultrathin sections of BCMV-PSt and PeMoV infected tissues, cytoplasmic cylindrical inclusions as well as filamentous virus particles were observed in the cytoplasm of parenchyma cells. ISEM revealed filamentous particles strongly decorated with antiserums of BCMV-PSt and PeMoV Peanut seeds were stained with BCMV-PSt and PeMoV antisera indicating the possibility of seed transmission far these viruses. Seedlings germinated from peanut seeds which reacted with antiserums of BCMV-PSt by DISA showed mild mottle or stripe symptoms while mosaic and necrotic mottle symptoms were observed for PeMoV-positive seedlings. Filamentous particles were strongly decorated with each antiserum under ISEM observation. BCMV-PSt coat protein gene of about 1.2 Kbp was amplified by RT-PCR. Altogether these results indicate that BCMV-PSt is the most prevalent virus infecting peanut in Korea.

• Journal of Wetlands Research
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• 제14권2호
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• pp.237-242
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• 2012

In order to determine the ecological characteristics of Quercus serrata and Q. aliena, which are potential natural vegetation of riverine in Korea, we cultivated the seedlings of two oak species under light, soil moisture and nutrient gradients from April to October in glasshouse. Then, we measured aboveground, belowground and total plant biomass and analyzed the differences in growth between two oak species. The two oak species showed decreasing growth with lower light intensity, but reduction in growth of Q. aliena was greater than that of Q. serrata. Q. serrata and Q. aliena had a constant growth state under soil moisture gradients, but Q. aliena grew well more than Q. serrata in lower soil moisture gradient. As soil nutrient availability decreased, the growth of Q. aliena was greater than Q. serrata. These results mean that the growth of two oak species is not affected by soil moisture, but the part of growth in Q. serrata may be positively affected in lower soil moisture condition. Also, Q. serrata has strong shade tolerance and the ability to adapt high nutrient condition relative to Q. aliena. Thus, Q. serrata and Q. aliena will be advantageous for the growth in stream ecosystem due to high light availability, but Q. serrata will be distributed near the lowlands by the water environment. Also, because the two oak species need high soil nutrient, it is important to preserve herbaceous vegetation to prevent nutrients that flowed in the streams.

• KOREAN JOURNAL OF CROP SCIENCE
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• 제53권3호
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• pp.239-243
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• 2008

This experiment was carried out to elucidate the appropriate split application method of nitrogen fertilizer in infant rice seedlings which show poor seedling growth resulted from extended seedling nursery duration more than 12 days. The 16 day-old infant rice seedling (DOIRS) showed poor seedling growth than 8 day-old seedling. Early growth of transplanted 16 DOIRS was poor, compared with that of transplanted 8 DOIRS. However, the poor plant growth estimated by plant height, tiller number, and the number of newly developed roots was compensated by 70-0-30% (basaltillering-panicle initiation) of nitrogen split application. In 70-0-30% plots among split nitrogen application methods, tiller number, biomass, and leaf area at heading stage of rice showed the highest values. Sixteen DOIRS plots showed lower grain filling and 1000-grain weight, resulted in lower yield of rice than 8 DOIRS plots in the same nitrogen split application. However the lower yield in 16 DOIRS plots was recovered by 70-0-30% of nitrogen split application method to a similar level in 8 DOIRS plots in which nitrogen was split applied by 40-30-30%.

• Horticultural Science & Technology
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• 제18권4호
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• pp.508-512
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• 2000

Growth of red pepper (Capsicum annuum) 'Kumtap', tomato (Lycoperisocon esculentum) 'Seokwang', petunia (Petunia hybrida) 'Madness Rose', and pansy (Viola tricolor) 'Magestic Giant' in mixtures of chestnut woodchips and granular rockwool at 25:75, 50:50, or 75:25 (v/v) was examined. Chestnut woodchips were soaked in water for 48 hours or aged in open field for 6 months in order to remove substances impeding plant growth. A commercial plug medium was used as the control. All treatments showed in a similar result in red pepper, petunia, and pansy. Plant height, fresh weight, dry weight, and chlorophyll content in media containing chestnut woodchips, especially in higher proportions, were poorer as compared to those in the control. On the contrary, height, fresh weight, and dry weight of tomato seedlings in media containing woodchips were significantly higher than those in the control. For petunia, pansy, and red pepper, six month ageing in open field of woodchips was more favorable for growth than 48 hour water soaking. Emergance of petunia seed was inferior, especially when woodchip content was higher, to the other crops with a resultant growth suppression. From the results, chestnut woodchips proved to be a practical material as a medium component only in tomato plants.

• Korean Journal of Fisheries and Aquatic Sciences
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• 제14권4호
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• pp.221-226
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• 1981

The seedlings of the scallop, Patinopecten yessoensis, which were collected in April 1980, April 1981 and May 1981, and the grown-up scallops collected in the sea bottom in November 1979, were reared by hanging method up to the July 1981. The growth of the seedlings collected in April 1980 was greatly affected by the time of the seedling collection, manipulation of the net cage, density in the net cage and the annual water temperature fluctuation. The scallops reared from the spat collected in April 1980 reached the sizes of 0.33 mm, 1.23 mm, 29.34 mm and 59.59 mm in shell length in 40,75, 285 and 450 days respectively. Since then, growth rate was determined as follows based on the age estimated by the year rings on the shell:84.96mm in 19 months, 99.3mm in 31, 112.3mm in 37 and 113.64mm in 43 months. The meat and the adductor muscle weight increased with the shell length. The meat weight roached about 15g when the shell length was 60-70mm and about 94.13 g when 130-140mm, and the adductor muscle weight reached about 4.89 g when the shell length was 60-70 mm and about 39.59g when 120-130 mm. But the growths of the meat and the adductor muscle weight were in stagnancy after scallops reached 125 mm in shell length.

• Korean Journal of Plant Resources
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• 제30권1호
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• pp.50-57
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• 2017

This study aimed to investigate the morphological characteristics and optimal germination conditions in seeds of Arabis pendula L., a traditionally edible and medicinal plant. The external seed shape was circular-obovate with narrow wings and dark brown. The seed length and width were 1.25 mm and 1.47 mm, respectively. The seeds were exalbuminous and the embryo was a bent type. Seed germination was the highest (49.7%) at $20^{\circ}C$ under dark conditions among the various temperature and light conditions applied. However, under the dark condition, the seedling was weak, overgrown, and the cotyledons were small and folded. To improve the germination and growth of seedlings, the seeds were pre-treated with $GA_3$ solutions of varying concentrations (0, 200, 500, and 1,000 mg/L). The seed germination and seedling growth were effectively improved by $GA_3$ pre-treatment. The germination rate was the highest (97.3%), mean germination time was the shortest (8.1 days), and a vigorous growth of seedlings was observed upon pre-soaking the seeds in 500 mg/L $GA_3$ solution. In conclusion, the best method for germination was pre-soaking in 500 mg/L $GA_3$ ($4^{\circ}C$, dark, 24 h) and incubating the seeds at $20^{\circ}C$ for 15 days.

• Journal of Radiation Protection and Research
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• 제26권4호
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• pp.409-415
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• 2001

To observe the stimulating effects of low dose gamma radiation on the germination and physiological activity of germinating seeds of old red pepper (Capsicum annuum L. cv Jokwang and cv. Hongkwang), seeds were irradiated at the dose of $2{\sim}50 Gy$. The germination rate of irradiation group was higher than that of the control. Especially it was highest at the early stage of induction. The germination rate at 7 days after sowing in Jokwang and Hongkwang cultivar was high as 74% and 11% at 4 Gy and 8 Gy irradiation group, respectively. The seedling height of Jokwang cultivar was noticeably high at 4 Gy irradiation group and that of Hongkwang cultivar at 8 Gy irradiation group. The protein contents of seedlings from seeds irradiated with low dose gamma radiation of Jokwang cultivar increased at the late stage of induction and that of Hongkwang cultivar at the early stage of induction. Catalase and peroxidase activities of seedlings from seeds irradiated with low dose gamma radiation of Jokwang cultivar increased at 4 Gy irradiation group and that of Hongkwang cultivar at 8 Gy irradiation group.

• Korean Journal of Plant Resources
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• 제21권5호
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• pp.347-351
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• 2008

Rhizomes of Acorus gramineus Soland have been used as sedatives, analgesics, and stomachics in Korean medicine. Even though A. gramineus produced in Korea is known as having better efficacy of a remedy than that in China, its cultivation area has not increased because of the lack of seedlings. To solve this problem, seed propagation method was tested. Seeds were harvested and sowed three times in June, 2005. The best results showed at greenish yellow seed stage harvested in mid June, and its germination ratio was 90.7%. Seeds were well stored at $5^{\circ}C$ for 30 days. As for in vitro culture, multiple shoots were induced first in MS basal medium supplemented with $2.0mg{\cdot}L^{-1}$ NAA plus $0.1mg{\cdot}L^{-1}$ BA, and then roots were induced in MS basal medium containing $0.1mg{\cdot}L^{-1}$ BA or $0.5mg{\cdot}L^{-1}$ NAA plus $1.0mg{\cdot}L^{-1}$ BA. Growth of A. gramineus seedlings in Wonyesangto, potting material sold in markets for horticultural plants, was superior to vermiculite.

• Journal of Life Science
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• 제22권12호
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• pp.1718-1723
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• 2012

Ten endophytic fungi with different colony morphologies were isolated from the roots of Suaeda japonica Makino growing naturally in Suncheon Bay. Plant growth promotion was verified by treating waito-c rice seedlings with culture filtrates from the endophytic fungi. The bioassays showed that the Sj/7/4 fungal strain induced effective growth promotion in the seedlings. The gibberellins (GA) produced by fungal strain Sj/7/4 were analyzed by gas chromatography /mass spectroscopy with selected ion monitoring (GC/MS SIM). The culture filtrate of Sj/7/4 fungal strain was confirmed to contain $GA_4$ through quantitative analysis. The Sj/7/4 fungal strain was identified to determine the internal transcribed spacer (ITS) regions with universal primers ITS-1 and ITS-4 by using polymerase chain reactions (PCR). Molecular analysis of the Sj/7/4 fungal strain showed high similarity to Fusarium solani. The Sj/7/4 fungal strain isolated from the root of S. japonica was therefore designated as F. solani Sj/7/4.

• Korean Journal of Medicinal Crop Science
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• 제1권2호
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• pp.120-124
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• 1993

Effect of eight different treatments to stimulate germination and of seedling ageson the growth and tuber yields of Liriope platyphlla was carried out in vitro(room temperature)and in vivo(field). Treatments, in vitro, of sulfuric acid(2%), $GA_3$(100ppm), and stratification were most stimulated the germination of L.platyphylla. Among them the stratification was also the most effective for emergence in field and was shortened the days to germination in vitro and in vivo. Plant height and the number of leaves and tillers per plant in the sulfuric acid and the vinyl mulch treatments were the poorest, but those in other treatments were highered without any differences. Seeding of L.platyphylla was taken 66 days to emerge and the growth of that was more decreased than that of the seedlings. The younger seedling, the shorter the day of emergence of a new shoot. The olders were bloomed earlier and more increased the growth than the yongers and the divided seedlings, but the tuber yield was most increased in the 3 years seedling.