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http://dx.doi.org/10.5423/RPD.2002.8.2.092

Bean common mosaic virus and Peanut mottle virus isolated from Peanut in Korea  

Koo, Dong-Jin (영남대학교 이과대학 생물학과)
Shin, Hye-Young (영남대학교 이과대학 생물학과)
Sung, Jung-Hyun (영남대학교 이과대학 생물학과)
Kang, Dong-Kyon (경북농업기술원 생물자원연구소)
Chang, Moo-Ung (영남대학교 이과대학 생물학과)
Publication Information
Research in Plant Disease / v.8, no.2, 2002 , pp. 92-100 More about this Journal
Abstract
For the survey of viruses infected in peanut cultivated in Korea, peanut seeds and leaves showing viral symptoms were collected from their growing areas. Typical symptoms on virus infected peanut leaves including mosaic, mottle with necrosis, yellowing, stripe or vein banding and stunts were observed. Two viruses isolated from the naturally infected peanuts were identified as Bean common mosaic virus(BCMV-PSt) and Peanut mottle virus(PeMoV) by their host range, immunosorbent elcetron microscopy(ISEM), direct immuno staining assay(DISA), RT-PCR, and intracellural symptoms. Direct negative staining method by electron microscope showed filamentous particles of about 780 m in length as well as inclusion bodies. In ultrathin sections of BCMV-PSt and PeMoV infected tissues, cytoplasmic cylindrical inclusions as well as filamentous virus particles were observed in the cytoplasm of parenchyma cells. ISEM revealed filamentous particles strongly decorated with antiserums of BCMV-PSt and PeMoV Peanut seeds were stained with BCMV-PSt and PeMoV antisera indicating the possibility of seed transmission far these viruses. Seedlings germinated from peanut seeds which reacted with antiserums of BCMV-PSt by DISA showed mild mottle or stripe symptoms while mosaic and necrotic mottle symptoms were observed for PeMoV-positive seedlings. Filamentous particles were strongly decorated with each antiserum under ISEM observation. BCMV-PSt coat protein gene of about 1.2 Kbp was amplified by RT-PCR. Altogether these results indicate that BCMV-PSt is the most prevalent virus infecting peanut in Korea.
Keywords
Arachis hypogaea; Bean common mosaic virus; Peanut mottle virus; RT-PCR;
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