Propagation of Acorus gramineus from Seeds and In vitro Culture

Rhizomes of Acorus gramineus Soland have been used as sedatives, analgesics, and stomachics in Korean medicine. Even though A. gramineus produced in Korea is known as having better efficacy of a remedy than that in China, its cultivation area has not increased because of the lack of seedlings. To solve this problem, seed propagation method was tested. Seeds were harvested and sowed three times in June, 2005. The best results showed at greenish yellow seed stage harvested in mid June, and its germination ratio was 90.7%. Seeds were well stored at $5^{\circ}C$ for 30 days. As for in vitro culture, multiple shoots were induced first in MS basal medium supplemented with $2.0mg{\cdot}L^{-1}$ NAA plus $0.1mg{\cdot}L^{-1}$ BA, and then roots were induced in MS basal medium containing $0.1mg{\cdot}L^{-1}$ BA or $0.5mg{\cdot}L^{-1}$ NAA plus $1.0mg{\cdot}L^{-1}$ BA. Growth of A. gramineus seedlings in Wonyesangto, potting material sold in markets for horticultural plants, was superior to vermiculite.

종자 및 기내배양을 이용한 석창포 증식

뿌리에 진통, 진정, 건위, 구충 효능이 있다고 알려진 석창포(Acorus gramineus Soland)의 확대재배를 위하여 현재 분근증식만으로 한정되어 있는 번식법을 개선하고자 종자번식과 기내배양에 의한 번식 방법을 검토하였다. 종자번식을 위한 수확적기는 종피가 연녹색을 띠었을 때가 적합하였으며, 종자의 보관은 $5^{\circ}C$에서 30일까지 저장하는 것이 발아율을 높게 유지할 수 있었다. 석창포의 기내발아에는 $2.0mg{\cdot}L^{-1}$ NAA와 $0.1mg{\cdot}L^{-1}$ BA를 MS 기본배지에 첨가하여 다수의 신초를 유도한 후, $0.1mg{\cdot}L^{-1}$ BA 단독 처리 및 $0.5mg{\cdot}L^{-1}$ NAA와 $1.0mg{\cdot}L^{-1}$ BA 혼합처리에서 발근을 유도하는 것이 효과적이었다. 석창포 육묘상토로는 vermiculite보다 원예상토가 생육이 우수하였다.