• Title/Summary/Keyword: yeast culture

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Effects of medium components on Mycelial Growth and Polysaccharide production in Liquid Culcure of Coriolus versicolor

  • Choi, Min-Gu;Hong, Eock-Kee
    • 한국생물공학회:학술대회논문집
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    • 2003.10a
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    • pp.253-257
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    • 2003
  • This study was executed to investgate the effects of medium components on liquid culture in the flask culture of Coriolus versicolor. This work was focused on raising the mycelial growth and the polysaccharide production. In order to optimize the medium, different carbon and nitrogen sources were investgated. Glucose and yeast extract were chosen for the production of mycelia and polysaccharide as carbon and nitrogen sources, respectively, in the flask culture. For the mycelia growth and polysaccharide production, the medium contained glucose 20g/L, yeast extract 6g/L, $KH_2PO_4$ 0.46g/L, $MgSO_4.7H_2O$ 0.5g/L. The liquid culture conditions for the mycelial growth were $27^{\circ}C$, 200rpm and working volume 100mL using 250mL flask.

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Optimum culture conditions of cell growth and polysaccharide production by Paecilomyces japonicain batch culture

  • Park, Seok-Jae;Byeon, Hak-Gyu;Han, Dae-Seok;Hong, Eok-Gi
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.287-290
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    • 2000
  • To examine effects of agitation and aeration as well as adding of glucose and yeast extract on cell growth and polysaccharide production by Paecilomyces japonica, batch culture was carried out at 5L jar fermenter at $27^{\circ}C$ with the initial pH 7 for 7 days cultivation(innoculum size 2%, working volume 3L). Media compositions(g/L) were 30 glucose, 20 yeast extract, 0.5 $KH_2PO_4$, $0.1\;CuCl_2\;{\cdot}\;2H_2O$. Optimum culture conditions of agitation and aeration in batch culture were 400 rpm and 1.0 vvm, resulting in 23.1 g/L biomass and 2.5 g/L polysaccharide. Additional feeding of glucose and yeast extract with a pulse mode conferred an advantage on cell growth and polysaccharide production with showing the results of 29.2 g/L and 3.3 g/L, respectively.

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Influence of Nutrient Addition in the Liquid Yeast Fermentation of Pulverized Food Wastes (남은 음식물의 습식효모배양에서 영양물질첨가가 효모증식에 미치는 영향)

  • Lee, Ki-Young;Yu, Sung-Jin;Chae, Hee-Jung
    • Journal of the Korea Organic Resources Recycling Association
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    • v.9 no.1
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    • pp.49-55
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    • 2001
  • For the production of probiotic feed enriched with viable yeasts, aerobic liquid culture of Kluyveromyces marxianus was attempted in pulverized residual food wastes. After the preliminary shaking culture result, the liquid food wastes was added with urea($0.5g/{\ell}$), o-phosphate($0.4g/{\ell}$ ), molasses($4g/{\ell}$), and yeast extract($1g/{\ell}$), and the fermentation was carried out in 2-litre jar fermenter. In 12 hours of aerobic mixed culture with Aspersillus oryzae, viable cell count of the yeast reached to the number of $1.4{\times}10^{10}/{\ell}$ in the cultured medium.

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Yeasts Associated with Roots of the Endemic Plant Mankyua chejuense

  • Kim, Jong-Shik;Kim, Dae-Shin;Jeon, Sang-Mi;Ko, Suk-Hyung
    • Korean Journal of Environmental Agriculture
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    • v.35 no.2
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    • pp.137-142
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    • 2016
  • BACKGROUND: Identification of endophytic yeasts inhabiting the internal roots of the Mankyua chejuense tree requires techniques involving biotechnology. There is a need for a culture-based method to isolate and identify yeast strains associated with M. chejuense.METHODS AND RESULTS: We spread homogenized M. chejuense root samples onto glucose-peptone- yeast agar containing antibiotics, Triton X-100, and L-sorbose. A total of 152 yeast isolates were obtained and identified via phylogenetic analysis based on ITS gene sequencing. The results revealed that the root-associated yeast species included the genera Cyberlindnera (140 isolates), Candida (11 isolates), and Kluyveromyces (one isolate). Additionally, three yeast isolates showed high bioethanol production.CONCLUSION: We identified the specific yeast community associated with M. chejuense roots. These yeast isolates may have industrial applications as bioethanol producers. Our findings revealed that Cyberlindnera isolates included C. suaverolens and C. satumus, while Kluyveromyces isolates showed high bioethanol production.

Yeasts in the Flowers of Wild Fleabane [Erigeron annus (L.) Pers.]

  • Kim, Jong-Shik;Kim, Dae-Shin
    • Korean Journal of Environmental Agriculture
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    • v.34 no.3
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    • pp.238-243
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    • 2015
  • BACKGROUND: Yeasts associated with fleabane flowers were identified using isolation methods previously applied in yeast biotechnology. A culture-based approach was required for isolation of many yeast strains associated with fleabane. METHODS AND RESULTS: We spread homogenized fleabane flowers onto GPY medium containing chloramphenicol, streptomycin, Triton X-100, and L-sorbose. We isolated 79 yeast strains from the flowers of wild fleabane, and identified the yeasts via phylogenetic analysis of isolates from agar plates. The yeast species included 39 isolates of Aureobasidium pullulans, 17 of the genus Candida, 14 of the genus Rhodosporidium, 6 of the genus Cryptococcus, and 3 of the genus Rhodotorula. CONCLUSION: Yeast isolates associated with fleabane flowers included A. pullulans (39 isolates) and other yeast species (40 isolates). Such yeast isolates may have biotechnological potential.

Studies on the Production of Microbial Culture Medium by Using By-Product of Salt-Fermented Kanary (까나리 액젓 부산물의 미생물 배지화를 위한 연구)

  • Won, Hye-Jin;Hahm, Young-Tae;Kim, Hye-Kyung;Kim, Byung-Yong
    • Applied Biological Chemistry
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    • v.43 no.3
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    • pp.202-206
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    • 2000
  • Feasibility of microbial culture media using by-product of salt fermented kanary was investigated. Gram negative strain, Escherichia coli, and Gram positive strain, Bacillus subtilis, and bioluminescent Photobacterium Phosphoreum were incubated with kanary by-Product media (KB media). Compared with LB media, KB media had enough carbon source, but lacked nitrogen source and growth factor. When 0.5% of peptone as a nitrogen source and 0.3% of yeast extract as nitrogen and growth factor source were fortified in KB media, the cell population rate was similar to LB media. Also, when 0.5% of yeast extract was fortified to KB media, it showed the same result as in LB media. The price of KB media with fortification of 0.5% peptone and 0.3% yeast extract, and 0.5% of yeast extract is only 46 and 19% of that of LB media, respectively. These results showed that kanary by-Product could be a good and cheaper bacterial culture media if small amount of nitrogen source and growth factor were added.

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Comprehensive Review of Indigenous Yeast Species in Korea: A Literature and Culture Collection Analysis (문헌 및 기탁 균주 현황분석을 통한 자생 효모 목록)

  • Ahn, Chorong;Kim, Minkyeong;Kim, Changmu
    • The Korean Journal of Mycology
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    • v.49 no.2
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    • pp.211-242
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    • 2021
  • Indigenous yeasts in Korea have been actively studied since 1910 in light of various fields, including environment, food fermentation, brewing, and medicine. However, a complete list of indigenous yeast species has not been addressed or reported. In this study, a comprehensive analysis of the literature including yeast isolation from Korean materials and the strains preserved at culture collections was performed. The results of this study indicate the following: i) there are 128 genera and 681 species (including 26 variants) of yeast reported in Korea, ii) the reported species were trimmed into 142 genera and 500 species (including 9 variants), iii) among which only 166 species were registered in the National Species List of Korea (2020). Therefore, follow-up studies are required to register more indigenous yeast species in the National Species List of Korea.

Studies on the Production of Yeast. (Part 1) Yeast Production from the Hydrolyzate of Sweet Potato Starch Cake as a Carbon Source (효모생산에 관한 연구(제1보) 고구마전분박 산당화액을 이용한 효모생산)

  • 양한철;최용진;성하진
    • Microbiology and Biotechnology Letters
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    • v.2 no.2
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    • pp.95-101
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    • 1974
  • Studies on the optimum conditions of acid hydrolysis of sweet potato starch cake and its utilization on the production of Saccharomyces cerevisiae as a carbon source were conducted and the results showed as follows; 1.The highest hydrolysis rate, 62.7 % of the reducing sugar based on the weight of the dry matter, was obtained when the starch cake was hydrolyzed with 1.0% of hydrochloric acid at 2.0 kg/$\textrm{cm}^2$ for 30 minutes. 2. But the yeast grew most favorably on the hydrolyzate obtained by treating the starch cake with 0.5% of hydrochloric acid at 2.0 kg/$\textrm{cm}^2$ for 10 minutes. Reducing sugar content of hydrolyzate was 51.4%. 3. The optimum pH of the culture medium was 7.0, Cell growth reached to the maximum at 36 hours of cultivation time. 4. According to the vitamin requirement tests, Ca-pantothenate was found to be a promoting factor for the growth of the yeast cells. 5. "Gluten acid hydrolyzate" was most effective to the cell growth when added to the medium at the concentration of 0.1% as a nitrogen source. 6. Sacch. cerevisiae could assimilate the sugars in the hydrolyzate about 89.1%, and the yields of the yeast cells showed 23.2mg/ml of culture medium.

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Biomass Production of Saccharomyces cerevisiae KFCC 10823 and Its Use in Preparation of Doenjang

  • Yoo, Jin-Young;Kim, Hyeon-Gyu;Kwon, Dong-Jin
    • Journal of Microbiology and Biotechnology
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    • v.7 no.1
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    • pp.75-80
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    • 1997
  • An ethanolic fermentation process was developed for preparing Doenjang with high ethanol. Higher and efficient viable cell production of salt-tolerant ethanolic yeast is a prerequisite for the successful commercial-scale process of ethanol production during Doenjang fermentation. Culture conditions of salt-tolerant yeast, S. cerevisiae KFCC 10823, was studied in terms of the effect of several environmental and nutritional factors. Viable cell numbers were the highest in a medium containing the following components per liter of water: soysauce, 300ml; dextrose, 50 g; beef extract, 5 g; yeast extract, 5 g; $KH_2PO_4$, 5 g; NaCl, 50 g. The optimal culture conditions of S. cerevisiae KFCC 10823 were pH 5.5, $25^{\circ}C$, 200 rpm and 0.5 vvm. Yeast viability during batch fermentation was gradually decreased to a level less than $90{\%}$ after 35 hours. The maximum cell number was $2.2{\times}10_7$ cells/ml at the optimal condition. Doenjang prepared with ethanolic yeast was ripened after 45 days at $30^{\circ}C$. This Doenjang contains 470 mg% of amino nitrogen and 2.5% ethanol. The shelf-life at $30^{\circ}C$ was theoretically estimated as 444 days.

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Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis

  • Bunsangiam, Sakaoduoen;Sakpuntoon, Varunya;Srisuk, Nantana;Ohashi, Takao;Fujiyama, Kazuhito;Limtong, Savitree
    • Mycobiology
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    • v.47 no.3
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    • pp.292-300
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    • 2019
  • IAA biosynthetic pathways in a basidiomycetous yeast, Rhodosporidiobolus fluvialis DMKU-CP293, were investigated. The yeast strain showed tryptophan (Trp)-dependent IAA biosynthesis when grown in tryptophan supplemented mineral salt medium. Gas chromatography-mass spectrometry was used to further identify the pathway intermediates of Trpdependent IAA biosynthesis. The results indicated that the main intermediates produced by R. fluvialis DMKU-CP293 were tryptamine (TAM), indole-3-acetic acid (IAA), and tryptophol (TOL), whereas indole-3-pyruvic acid (IPA) was not found. However, supplementation of IPA to the culture medium resulted in IAA peak detection by high-performance liquid chromatography analysis of the culture supernatant. Key enzymes of three IAA biosynthetic routes, i.e., IPA, IAM and TAM were investigated to clarify the IAA biosynthetic pathways of R. fluvialis DMKU-CP293. Results indicated that the activities of tryptophan aminotransferase, tryptophan 2-monooxygenase, and tryptophan decarboxylase were observed in cell crude extract. Overall results suggested that IAA biosynthetic in this yeast strain mainly occurred via the IPA route. Nevertheless, IAM and TAM pathway might be involved in R. fluvialis DMKU-CP293.