Browse > Article
http://dx.doi.org/10.1080/12298093.2019.1638672

Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis  

Bunsangiam, Sakaoduoen (Department of Microbiology, Faculty of Science, Kasetsart University)
Sakpuntoon, Varunya (Department of Microbiology, Faculty of Science, Kasetsart University)
Srisuk, Nantana (Department of Microbiology, Faculty of Science, Kasetsart University)
Ohashi, Takao (International Center for Biotechnology, Osaka University)
Fujiyama, Kazuhito (International Center for Biotechnology, Osaka University)
Limtong, Savitree (Department of Microbiology, Faculty of Science, Kasetsart University)
Publication Information
Mycobiology / v.47, no.3, 2019 , pp. 292-300 More about this Journal
Abstract
IAA biosynthetic pathways in a basidiomycetous yeast, Rhodosporidiobolus fluvialis DMKU-CP293, were investigated. The yeast strain showed tryptophan (Trp)-dependent IAA biosynthesis when grown in tryptophan supplemented mineral salt medium. Gas chromatography-mass spectrometry was used to further identify the pathway intermediates of Trpdependent IAA biosynthesis. The results indicated that the main intermediates produced by R. fluvialis DMKU-CP293 were tryptamine (TAM), indole-3-acetic acid (IAA), and tryptophol (TOL), whereas indole-3-pyruvic acid (IPA) was not found. However, supplementation of IPA to the culture medium resulted in IAA peak detection by high-performance liquid chromatography analysis of the culture supernatant. Key enzymes of three IAA biosynthetic routes, i.e., IPA, IAM and TAM were investigated to clarify the IAA biosynthetic pathways of R. fluvialis DMKU-CP293. Results indicated that the activities of tryptophan aminotransferase, tryptophan 2-monooxygenase, and tryptophan decarboxylase were observed in cell crude extract. Overall results suggested that IAA biosynthetic in this yeast strain mainly occurred via the IPA route. Nevertheless, IAM and TAM pathway might be involved in R. fluvialis DMKU-CP293.
Keywords
Indole-3-acetic acid; IAA biosynthesis; basidiomycetous yeast; Rhodosporidiobolus fluvialis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 El-Tarabily KA. Suppression of Rhizoctonia solani diseases of sugar beet by antagonistic and plant growth-promoting yeasts. J Appl Microbiol. 2004;96:69-75.   DOI
2 Nutaratat P, Srisuk N, Arunrattiyakorn P, et al. Indole-3-acetic acid biosynthetic pathways in the basidiomycetous yeast Rhodosporidium paludigenum. Arch Microbiol. 2016;198:429-437.   DOI
3 Rao RP, Hunter A, Kashpur O, et al. Aberrant synthesis of indole-3-acetic acid in Saccharomyces cerevisiae triggers morphogenic transition, a virulence trait of pathogenic fungi. Genetics. 2010;185:211.   DOI
4 Sun P-F, Fang W-T, Shin L-Y, et al. Indole-3-acetic acid-producing yeasts in the phyllosphere of the carnivorous plant Drosera indica L. Plos One. 2014;9:e114196.   DOI
5 Surussawadee J, Khunnamwong P, Srisuk N, et al. Papiliotrema siamense f.a., sp. nov., a yeast species isolated from plant leaves. Int J Syst Evol Microbiol. 2014;64:3058-3062.   DOI
6 Nutaratat P, Amsri W, Srisuk N, et al. Indole-3-acetic acid production by newly isolated red yeast Rhodosporidium paludigenum. J Gen Appl Microbiol. 2015;61:1-9.   DOI
7 Mujahid M, Sasikala C, Ramana CV. Production of indole-3-acetic acid and related indole derivatives from L-tryptophan by Rubrivivax benzoatilyticus JA2. Appl Microbiol Biotechnol. 2011;89:1001-1008.   DOI
8 Kulkarni GB, Sanjeevkumar S, Kirankumar B, et al. Indole-3-acetic acid biosynthesis in Fusarium delphinoides strain GPK, a causal agent of wilt in chickpea. Appl Biochem Biotechnol. 2013;169:1292-1305.   DOI
9 Tanaka E, Tanaka C, Ishihara A, et al. Indole-3-acetic acid biosynthesis in Aciculosporium take, a causal agent of witches' broom of bamboo. J Gen Plant Pathol. 2003;69:1-6.   DOI
10 Ricardo C-L, Campos-Reales N, Elmerich C, et al. Physiological evidence for differently regulated tryptophan-dependent pathways for indole-3-acetic acid synthesis in Azospirillum brasilense. Mol Gen Genet. 2000;264:521-530.   DOI
11 Reineke G, Heinze B, Schirawski J, et al. Indole-3-acetic acid (IAA) biosynthesis in the smut fungus Ustilago maydis and its relevance for increased IAA levels in infected tissue and host tumour formation. Mol Plant Pathol. 2008;9:339-355.   DOI
12 Hilbert M, Voll LM, Ding Y, et al. Indole derivative production by the root endophyte Piriformospora indica is not required for growth promotion but for biotrophic colonization of barley roots. New Phytol. 2012;196:520-534.   DOI
13 Krause K, Henke C, Asiimwe T, et al. Biosynthesis and secretion of indole-3-acetic acid and its morphological effects on Tricholoma vaccinum-spruce ectomycorrhiza. Appl Environ Microbiol. 2015;81:7003-7011.   DOI
14 Mashiguchi K, Tanaka K, Sakai T, et al. The main auxin biosynthesis pathway in Arabidopsis. Proc Natl Acad Sci USA. 2011;108:18512-18517.   DOI
15 Zhao Y. Auxin biosynthesis. Arabidopsis book. 2014;12:e0173.   DOI
16 Brandi M, Clark EM, Lindow SE. Characterization of the indole-3-acetic acid (IAA) biosynthetic pathway in an epiphytic strain of Erwinia herbicola and IAA production in vitro. Can J Microbiol. 1996;42:586-592.   DOI
17 Mano Y, Nemoto K. The pathway of auxin biosynthesis in plants. J Exp Bot. 2012;63:2853-2872.   DOI
18 Libbert E, Risch H. Interactions between plants and epiphytic bacteria regarding their auxin metabolism: v. isolation and identification of the IAA-producing and destroying bacteria from pea plants. Physiol Plant. 1969;22:51-58.   DOI
19 Ruanpanun P, Tangchitsomkid N, Hyde KD, et al. Actinomycetes and fungi isolated from plant-parasitic nematode infested soils: screening of the effective biocontrol potential, indole-3-acetic acid and siderophore production. World J Microbiol Biotechnol. 2010;26:1569-1578.   DOI
20 Limtong S, Koowadjanakul N. Yeasts from phylloplane and their capability to produce indole-3-acetic acid. World J Microbiol Biotechnol. 2012;28:3323-3335.   DOI
21 Spaepen S, Vanderleyden J, Remans R. Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS Microbiol Rev. 2007;31:425-448.   DOI
22 Duca D, Lorv J, Patten CL, et al. Indole-3-acetic acid in plant-microbe interactions. Antonie Van Leeuwenhoek. 2014;106:85-125.   DOI
23 Zakharova EA, Shcherbakov AA, Brudnik VV, et al. Biosynthesis of indole-3-acetic acid in Azospirillum brasilense. Insights from quantum chemistry. Eur J Biochem. 1999;259:572-576.   DOI
24 Xin G, Glawe D, Doty SL. Characterization of three endophytic, indole-3-acetic acid-producing yeasts occurring in Populus trees. Mycol Res. 2009;113:973-980.   DOI
25 Limtong S, Kaewwichian R, Yongmanitchai W, et al. Diversity of culturable yeasts in phylloplane of sugarcane in Thailand and their capability to produce indole-3-acetic acid. World J Microbiol Biotechnol. 2014;30:1785-1796.   DOI
26 Koshiba T, Saito E, Ono N, et al. Purification and properties of flavin- and molybdenum-containing aldehyde oxidase from coleoptiles of maize. Plant Physiol. 1996;110:781-789.   DOI
27 Stes E, Prinsen E, Holsters M, et al. Plant-derived auxin plays an accessory role in symptom development upon Rhodococcus fascians infection. Plant J. 2012;70:513-527.   DOI
28 Koga J, Adachi T, Hidaka H. IAA Biosynthetic pathway from tryptophan via indole-3-pyruvic acid in Enterobacter cloacae. Agric Biol Chem. 1991;55:701-706.   DOI
29 Brown HM, Purves WK. Indole acetaldehyde reductase of Cucumis sativus L: kinetic properties and role in auxin biosynthesis. Plant Physiol. 1980;65:107-113.   DOI
30 Zhao Y. Auxin biosynthesis: a simple two-step pathway converts tryptophan to indole-3-acetic acid in plants. Mol Plant. 2012;5:334-338.   DOI
31 Fu S-F, Sun P-F, Lu H-Y, et al. Plant growth-promoting traits of yeasts isolated from the phyllosphere and rhizosphere of Drosera spatulata Lab. Fungal Biol. 2016;120:433-448.   DOI
32 Spaepen S, Vanderleyden J. Auxin and plantmicrobe interactions. Cold Spring Harb Perspect Biol. 2011;3:a001438.   DOI
33 Nutaratat P, Srisuk N, Arunrattiyakorn P, et al. Plant growth-promoting traits of epiphytic and endophytic yeasts isolated from rice and sugar cane leaves in Thailand. Fungal Biol. 2014;118:683-694.   DOI