• Title/Summary/Keyword: wood column

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Phenolic Glycosides from Cercidiphyllum japonicum Leaves

  • Lee, Tae-Seong;Min, Hee-Jeong;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.5
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    • pp.591-599
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    • 2015
  • Cercidiphyllum japonicum leaves were collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, methylene chloride ($CH_2Cl_2$), ethylacetate (EtOAc), and $H_2O$. A portion of EtOAc fraction (10 g) was chromatographed on a Sephadex LH-20 column, by the successively elution with various aqueous $MeOH-H_2O$ (1:9, fraction $1-2{\rightarrow}3:7$, fraction $3-5{\rightarrow}1:1$, fraction $6-9{\rightarrow}7:3$, fraction $10-13{\rightarrow}9:1$, fraction 14-16). Compound 2 was isolated from fraction 6 and compound 1 was separated from fraction 11 and 12. Compound 3 and 4 were purified from fraction 13. The isolated compounds were elucidated as quercetin-3-O-${\alpha}$-L-rhamnopyranoside (1), chlorogenic acid (2), quercetin-3-O-${\alpha}$-L-arabinofuranoside (3) and quercetin-3-O-${\beta}$-D-xylopyranoside (4) by the spectral and literature data, and by comparison with the authentic samples. These compounds were reported, for the first time, from the extracts of C. japonicum leaves. Also chlorogenic acid (2) has never been reported before in domestic tree species and can be used as an index compound for C. japonicum.

A Polyoxygenated Ellagitannin from Cercidiphyllum japonicum Bark

  • Lee, Min-Sung;Min, Hee-Jeong;Kim, Jin-Kyu;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.4
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    • pp.551-558
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    • 2016
  • Katsura tree (Cercidiphyllum japonicum Sieb. Et Zucc) bark was collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, methylene chloride ($CH_2Cl_2$), ethylacetate (EtOAc), and $H_2O$. The $H_2O$ fraction was chromatographed on a Sephadex LH-20 column with various aqueous MeOH eluting solvents to isolate an ellagitannin. The isolate was elucidated as macabarterin, a polyoxygenated ellagitannin by NMR analysis, including HSQC, HMBC, Q-TOF MS, and with the comparison of authentic literature data. The compound was an ellagitannin which was isolated, for the first time, from the extracts of Katsura tree bark, and has never been reported before in domestic tree or plant sources.

Hydrolysable Tannins from Cercidiphyllum japonicum Bark

  • Lee, Min-Sung;Min, Hee-Jeong;Si, Chuan-Ling;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.4
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    • pp.559-570
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    • 2016
  • The EtOAc and $H_2O$ soluble fractions of Katsura tree (Cercidiphyllum japonicum Sieb. Et Zucc) bark extracts were chromatographed on a Sephadex LH-20 column with various aqueous MeOH. Gallic acid (1), methyl galate (2), kurigalin (3), 1,2,3,6-tetra-O-galloyl-${\beta}$-D-glucose (4) and 1,2,3,4,6-penta-O-galloyl-${\beta}$-D-glucose (5) were isolated from EtOAc fraction. Isocorilagin (6) and methyl galate (2) were separated from $H_2O$ fraction. The structure determination was done by $^1H$ and $^{13}C$ NMR. Of these isolated compounds, methyl galate (2), kurigalin (3) and isocorilagin (6) were isolated, for the first time, from the bark extracts of Cercidiphyllum japonicum.

Procyanidins from Acer komarovii Bark

  • Lee, Tae-Sung;Kwon, Dong-Joo;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.2
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    • pp.214-223
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    • 2015
  • The bark of Acer komarovii was collected, ground, and extracted with 70% aqueous acetone to obtain concentrates. The concentrates were suspended in $H_2O$, and then successively partitioned with n-hexane, dichloromethane and ethylacetate to be freeze dried. A portion of ethylacetate fraction was chromatographed on a Sephadex LH-20 and a RP C-18 column with various aqueous MeOH-$H_2O$ (1:0, 1:1, 1:2, 1:5, 1:7, 1:9, 1:10, 3:1, and 4:1, v/v) eluents. Four compounds were isolated; (-)-epicatechin (9.6 g), procyanidin A2 (epicatechin-($4{\beta}{\rightarrow}8$, $2{\beta}{\rightarrow}O{\rightarrow}7$)-epicatechin) (1.3 g), procyanidin B2 (epicatechin-($4{\beta}{\rightarrow}8$)-epicatechin) (40.0 mg), and cinnamtannin B1 (epicatechin-($4{\beta}{\rightarrow}8$, $2{\beta}{\rightarrow}O{\rightarrow}7$)-epicatechin-($4{\beta}{\rightarrow}8$)-epicatechin) (690 mg). The analysis of the bark procyanidins showed that the basic unit constituting condensed tannins was only (-)-epicatechin. This study, for the first time, report the procyanidins of Acer komarovii bark.

Studies on Biological Activity of Wood Extractives(XVI) -Antioxidant Components from the Bark of Rbus chinensis-

  • Lee, Yeon-Suk;Park, Youngki;Lee, Oh-Kyu;Park, Il-Kwon;Shin, Sang-Chul;Kang, Ha-Young;Choi, Don-Ha;Choi, Tae-Ho;Lee, Hak-Ju
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.5 s.133
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    • pp.86-91
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    • 2005
  • Six compounds were isolated from the EtOAc and $Et_2O$ fractions of the bark of Rhus chinensis by repeated column chromatography with $SiO_2$ and Sephadex LH-20. The structures were determined by instrumental analysis using MS and NMR spectrophotometer as: gallic acid (1), methyl gallate (2), 6, 7-dimethoxycoumarin (3), orcinol-${\beta}$-D-glucoside (4), scopoletin (5), semialactone (6). Among these compounds, 6,7-dimethoxycoumarin (3) was isolated from this plant for the first time. To measure the antioxidant activity, the DPPH radical scavenging activity test was performed. Gallic acid (1) showed the strongest activity, while orcinol-${\beta}$-D-glucoside (4), semialactone (5) and scopoletin (6) had the low activities.

Apigenin Derivatives of Paulownia coreana Uyeki Leaves

  • Si, Chuan-Ling;Kim, Jin-Kyu;Kwon, Dong-Joo;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.2
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    • pp.83-87
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    • 2006
  • The leaves of Paulownia coreana Uyeki were extracted with acetone-$H_2O$ (7:3, v/v), concentrated under reduced pressure and fractionated successively with n-hexane, methylene chloride and ethyl acetate, leaving residual water soluble fraction. A portion of the resulting aqueous soluble powder was chromatographed on a Sephadex LH-20 column using aqueous methanol and ethanol-hexane as washing solvents. Three apigenin derivatives were isolated and identified as apigenin-7-O-${\beta}$-D-glucpyranoside, apigenin-7-O-${\beta}$-D-glucuronopyranoside and apigenin-7-O-[${\beta}$-D-glucuronopyranosyl($1{\rightarrow}2$)-O-${\beta}$-D-glucuronopyranoside] by spectroscopic methods including NMR and FAB-MS.

Phenylpropanoid Glycosides of Paulownia coreana Uyeki Leaves

  • Si, Chuan-Ling;Kim, Jin-Kyu;Kwon, Dong-Joo;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.2
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    • pp.78-82
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    • 2006
  • The leaves of Paulownia coreana Uyeki were collected, extracted with acetone-$H_2O$ (7:3, v/v), concentrated under reduced pressure and successively fractionated using n-hexane, methylene chloride, ethyl acetate and water on a separatory funnel. A portion of the ethyl acetate soluble powder was chromatographed on a Sephadex LH-20 column using aqueous methanol and ethanol-hexane as washing solvents. Two isomeric phenylpropanoid glycosides were isolated and elucidated as verbascoside and isoverbascoside by NMR and MS spectrometers.

Isolation and Identification of Stilbene glycosides from the Bark of Pinus koraiensis (잣나무 수피의 Stilbene glycosides의 분리 및 동정)

  • Song, Hong-Keun
    • Journal of the Korean Wood Science and Technology
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    • v.29 no.4
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    • pp.97-102
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    • 2001
  • EtOAc extract from the bark of Pinus koraiensis Sieb. et Zucc was isolated by column chromatography which was packed with Sephadex LH-20 or TSK-gel HW-40F. Several stilbene glycosides were identified by $^1H{\cdot}^{13}C$-NMR, HMQC, HMBC and $FAB^+$ MS. Three stilbene glycosides, Z-pinostilbenoside, E-desoxyrhaponticin, and E-resveratroloside, were identified.

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Study on Damage of Column of Wooden Traditional Building by Insects (곤충에 의한 목조 고 건축물 기둥의 손상에 대한 연구)

  • Kim, Gwang-Chul;Jeong, In-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.31 no.2
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    • pp.69-76
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    • 2003
  • Front columns of buildings were most severely damaged by insects. Hole diameter damaged by insects were different in each location on columns, that is, bottom, middle position, or top of columns. That mean various insects attacked on columns of wooden traditional buildings because kinds of each insect depends on the hole diameter. Specially, middle position of columns were harmed seriously. Hole diameters damaged by insects were various from 1 mm to 10 mm, but approximately 5 mm diameter was found most commonly.

Continuous Ethanol Fermentation using Immobilized Yeasts (고정화 효모에 의한 연속적 에탄올 발효)

  • 서근학;송승구;문성훈
    • Microbiology and Biotechnology Letters
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    • v.14 no.2
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    • pp.199-203
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    • 1986
  • A tubular tormentor was prepared by packing the wood chips and pumping the yeast solution of Saccharomyces formosensis in a tubular column. Investigations to characterize the ethanol fermentation in the immobilized cell tubular fermentor and to compare such a fermentors with other type fermentors were undertaken. Ethanol productivity of 24.4g EtOH/$\ell$.hr has been obtained from glucose substrate. This productivity is higher or compared favourably with that reported in immobilized bio-reactors.

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