• Biotechnology and Bioprocess Engineering:BBE
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• 제10권2호
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• pp.155-161
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• 2005

The inherent instability of metabolite production in plant cell culture-based bioprocessing is a major problem hindering its commercialization. To understand the extent and causes of this instability, this study was aimed at understanding the variability of anthocyanin accumulation during long-term subcultures, as well as within subculture batches, in Vitis vinifera cell cultures. Therefore, four cell line suspensions of Vitis vinifera L. var. Gamay Freaux, A, B, C and D, originated from the same callus by cell-aggregate cloning, were established with starting anthocyanin contents of $2.73\;\pm\;0.15,\;1.45\;\pm\;0.04,\;0.7\;\pm\;0.024\;and\;0.27\;\pm\;0.04$CV (Color Value)/g-FCW (fresh cell weight), respectively. During weekly subculturing of 33 batches over 8 months, the anthocyanin biosynthetic capacity was gradually lost at various rates, for all four cell lines, regardless of the significant difference in the starting anthocyanin content. Contrary to this general trend, a significant fluctuation in the anthocyanin content was observed, but with an irregular cyclic pattern. The variabilities in the anthocyanin content between the subcultures for the 33 batches, as represented by the variation coefficient (VC), were 58, 57, 54, and $84\%$ for V. vinifera cell lines A, B, C and D, respectively. Within one subculture, the VCs from 12 replicate flasks for each of 12 independent subcultures were averaged, and found to be $9.7\%$, ranging from 4 to $17\%$. High- and low-producing cell lines, VV05 and VV06, with 1.8-fold differences in their basal anthocyanin contents, exhibited different inducibilities to L-phenylalanine feeding, methyl jasmonate and light irradiation. The low-producing cell line showed greater potential in enhanced the anthocyanin production.

• 한국작물학회지
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• 제64권2호
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• pp.89-101
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• 2019

The variation of content of 12 soybean seed isoflavone components was determined in the aglycone, glucoside, malonylglucoside and acetylglucoside groups of 44 Korean soybean cultivars grown in 2016 as well as in 2017. The total isoflavone content of the 44 cultivars averaged at $2935.4{\mu}g/g$ and was in the range of 950.6 to $5226.3{\mu}g/g$ for two years. Malonylglucoside group averaged at $2437.2{\mu}g/g$ with the highest proportion of isoflavone composition (83.0%). Significant differences were observed between cultivars, years and their interactions for both the total isoflavone and each composition group contents (P < 0.0001); however, no year-wise differences were observed for daidzein and genistin. The broad-sense heritability ($h^2$) within the set of 44 Korean soybean cultivars was as high as 0.93 for the total isoflavone content and was in the range of 0.8-0.92 for each composition group of isoflavone except for acetylglucoside. The total isoflavone content in cultivar group for soy-sprout was higher ($3850.4{\mu}g/g$) than that for the other cultivar groups of soy-paste and tofu ($3082.8{\mu}g/g$), black or green soybean cooked with rice ($2345.8{\mu}g/g$), and early maturity group ($1298.6{\mu}g/g$). The total isoflavone content of 'Sowonkong', a soybean cultivar for soy-sprout, was the highest ($5226.3{\mu}g/g$). In the cultivar group for soy-paste and tofu, the average isoflavone contents of 'Daepung', 'Daepung2ho', 'Saegeum', 'Uram', and 'Jinpung' were higher than $4000{\mu}g/g$. With the exception of small seeded cultivars with low isoflavone contents such as 'Sohwang' and 'Socheongja', the seed size and total isoflavone content were significantly negatively correlated in 2016 and 2017, respectively ($r=-0.47^{**}$ and $-0.49^{**}$). The number of days of growth from flowering to maturity did not affect the variations observed in isoflavone content.

• 대한치과교정학회지
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• 제15권1호
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• pp.123-140
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• 1985

Variation in the facial pattern and effect of the rotational jaw growth on the facial proportion were studied in serial cephalometric radiographs of 40 Korean children (25 boys, 15 girls) ranging in age from 6 to 13 years. According to Y-axis (N-S-Gn) growth change during the 7 years of period, the subject who had exhibited 'wave-like' manner of Y-axis growth change within ${\pm}\;2^{\circ}$ was classified to the Parallelwise group and the subject who had exhibited Y-axis increase more than $+2^{\circ}$ was classified to the Clockwise rotation group and the subject who had exhibited Y-axis decrease more than $-2^{\circ}$ was classified to the Counterclockwise rotation group. For the comparison of each group, a total of 22 morphologic variables were employed and the data were analyzed by means of computer morphometrics and statistical methods. On the basis of the finding of this study, the following trends were established. 1. The Parallelwise group was $75\%$, the Clockwise rotation group was $12.5\%$ and the Counter-clockwise rotation group was $12.5\%$. 2. The growth pattern of cranial base was related to the rotation of mandible. 9. Maxillary prognathism was occured in the Counterclockwise rotation group and the rotation of palatal plane was occured in the Clockwise rotation group. 4. Mandibular prognathism was occured in the Parallelwise and the Counterclockwise rotation groups, especially in the Counterclockwise rotation group. 5. The degree of maxillo-mandibular divergency was constant in the Clockwise rotation group but decreased in the Counterclockwise rotation and the Parallelwise groups, especially in the Counterclockwise group. 6. There were no differences in the size of the anterior upper facial height (N-ANS) and the posterior lower facial height ((Go-Me)-PNS) but there were differences in the size of the anterior lower facial height (ANS-Me) and the posterior upper facial height ((S-N)-PNS) between each group. 7. The growth increment and the size of the facial depth was not related to the growth pattern of the face but the growth increment and the size of the facial height was related to the growth pattern of the face. 8. Proportional change due to the facial growth rotation was concentrated in the anterior lower face. 9. The most apparent difference between each group was happened in the size of the posterior cranial base (S-Ba) and the facial length (S-Gn).

• 한국작물학회지
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• 제37권6호
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• pp.521-527
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• 1992

우리나라 각 지역에서 수집한 유색대두 계통에 관한 당함량변이와 그의 주요 특성과의 관계를 살펴 본 결과를 요약하면 다음과 같다. 1. 유색 대두 수집계통의 당함량의 변이는 최저 8.3%에서 최고 12.1%의 범위로서 평균 10.1%를 나타내었다. 2. 국내 지방수집 유색대두 1087계통에서 성숙군 분포는 O군 (--115일) 0.5%, 1군(116--123일) 1%, II군(124--131일) 5.7%, III군(132--139일) 16%, Ⅵ군(140-147일) 19%, V군(148--155일) 22%, Ⅵ군(156--163일) 14% 그리고 군(164--일) 19%로 나타났고, 대부분 III, IV, V, Ⅵ, Ⅶ군에 속하는 중ㆍ만생계통인 것으로 나타났으며 만생종일수록 당함량이 많은 경향이었다. 그리고 각각의 지역에 있어서 성숙일수가 긴 성숙군 계통들의 당함량이 높은 것으로 나타났다. 3. 종피색에 따른 당함량의 차이는 볼 수 없었고, 피분유무에 따른 당함량의 차이는 피분이 있는 계통이 높은 것으로 나타났다. 4. 유색대두 종실의 자엽색에 따른 당함량의 변이는 노란 자엽계통보다 녹색 자엽 계통이 높은 것으로 나타났다. 5. 전체 수결 계통에서 당함량은 단백질함량과는 부의 상관(-029$^{**}$ )을, 지방함량과는 정의 상관(0.21$^{**}$ )을, 고리고 성숙기간과는 정의 상관(0.36$^{**}$ )을 나타내었고, 각 성숙군내에서도 조숙계통을 제외한 각 성숙군 내 계통들에서 당함량은 단백질 함량 및 지방함량과 유의 상관을 나타내었다. 또한 당함량과 알카리 붕괴도 간에도 유의적 정의 상관을 나타내었다.

• Journal of Oral Medicine and Pain
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• 제14권1호
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• pp.57-66
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• 1989

66 patients with temporomandibular disorders were selected for experimental group, and 45 normal subjects who were Dental students were selected for control group. Average age of experimental group was 30.5 years, Male to Female ratio was 2 : 3, and their age distribution were teen-ages to seventh decades. Transcranial radiography (TR) with Denar Accurad 100 was used for each group to get the values of width in joint spaces and to investigate the bony changes of articular surfaces and relative condylar position to articular fossa. In addition to TR, clinical interview and routine charting about amount of mandibular movements and occlusal variations were carried out in experimental group. The obtained results were as follows : 1. The mean values of joint space with in control group were 2.15mm to anterior, 2.98mm to superior and 2.29mm to posterior and the value of relative condylar position to the deepest portion of articular fossa was 0.21mm to anterior. In experimental group, those values were 2.01mm, 2.14mm 2.22mm and 0.12mm to posterior in sequence, respectively. Joint spaces in experimental group, therefore, were inclined to decreased, and relative condylar position was inclined to retrude. Joint space in control group showed symmetric condylar position, but in experimental group showed asymmetry. 2. Non-affected joints with no bony changes in experimental group showed the narrowest joint spaces which were thought to manifest the abnormal stress to non-affected side to dysfunctional state of contralateral affected joints. 3. Amount of mandibular movements in experimental group were within normal values in lateral movements and in protrusive movement but in opening movement with or without passive stretch, those were lower than normal values. Frequency of occlusal variation, for example, protrusive posterior contacts, open bite, median line shift to lateral side were inclined to increase with bony changes and with crepitus.

• Asian-Australasian Journal of Animal Sciences
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• 제15권8호
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• pp.1178-1181
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• 2002

Records from a total of 202 crossbred pigs were classified by their ear type and coat color to examine the presence of interrelationships with growth performances. Crossbred pigs were F2 generations of full sib family out of ten Landrace sows bred by 5 Korean domestic boars. Heavily drooped ear type was predominant, 195 out of 202 pigs over the other two types (1 straight and 6 slightly drooped). Coat colors were classified as four categories, all white, all black, dominant white or dominant black. Ratio among coat color categories did not fall within Mendelian principle of independence regarding two loci involved. There was dependency between ear type and coat color. However, due to rarity of ear types other than heavy drooped, dependency comes from distribution of those rare ear types. Three least squares models to test the effect of ear type and coat colors on growth performances were analyzed. First model analyzed effects on birth weight, body weight at 3 and 6 weeks and ADG' before weaning and between 3 and 5 weeks of age. This model included sex in addition to ear type and coat color. Second model analyzed postweaning growth traits (initial weight, final weight and ADG between these periods) upon initiation of performance testing. This model included effects of sex, test group and start age (as a covariate) in addition. Third model was fit for fasted weight before slaughter and included the effects of sex, test group and age at slaughter (as a covariate). The effects of sex and ear type were not significant source of variation for all traits. Test group was a significant source of variation for all the postweaning traits. Effect of coat color was not significant until the initiation of performance testing and became significant then after. Least squares means of dominantly black pigs were significantly lower than the other three coat colored pigs in final weight around 195 days of age and in ADG from the start of performance test and final weight measure.

• Animal Systematics, Evolution and Diversity
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• 제27권1호
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• pp.1-17
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• 2011

Thais Roding, 1798, commonly known as rock-shell, is among the most frequently found gastropod genera worldwide on intertidal rocky shores including those of Japan, China, Taiwan and Korea. This group contains important species in many marine environmental studies but species-level taxonomy of the group is quite complicated due to the morphological variations in shell characters. This study examined the genetic variations and relationships among three Korean Thais species based on the partial nucleotide sequences of mitochondrial cox1 gene fragments. Phylogenetic trees from different analytic methods (maximum parsimony, neighbor-joining, and maximum likelihood) showed that T. bronni and T. luteostoma are closely related, indicating the most recent common ancestry. The low sequence divergence found between T. luteostoma and T. bronni, ranging from 1.53% to 3.19%, also corroborates this idea. Further molecular survey using different molecular marker is required to fully understand a detailed picture of the origin for their low level of interspecific sequence divergence. Sequence comparisons among conspecific individuals revealed extensive sequence variations within the three species with maximum values of 2.43% in T. clavigera and 1.37% in both T. bronni and T. luteostoma. In addition, there is an unexpectedly high level of mitochondrial genotypic diversity within each of the three Korean Thais species. The high genetic diversity revealed in Korean Thais species is likely to reflect genetic diversity introduced from potential source populations with diverse geographic origins, such as Taiwan, Hong Kong, and a variety of different coastal regions in South China and Japan. Additional sequence analysis with comprehensive taxon sampling from unstudied potential source populations will be also needed to address the origin and key factors for the high level of genetic diversity discovered within the three Korean Thais species studied.

• The Plant Pathology Journal
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• 제39권2호
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• pp.171-180
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• 2023

Spring black stem and leaf spot, caused by Phoma medicaginis, is an issue in annual Medicago species. Therefore, in this study, we analyzed the response to P. medicaginis infection in a collection of 46 lines of three annual Medicago species (M. truncatula, M. ciliaris, and M. polymorpha) showing different geographic distribution in Tunisia. The reaction in the host to the disease is explained by the effects based on plant species, lines nested within species, treatment, the interaction of species × treatment, and the interaction of lines nested within species × treatment. Medicago ciliaris was the least affected for aerial growth under infection. Furthermore, the largest variation within species was found for M. truncatula under both conditions. Principal component analysis and hierarchical classification showed that M. ciliaris lines formed a separate group under control treatment and P. medicaginis infection and they are the most vigorous in growth. These results indicate that M. ciliaris is the least susceptible in response to P. medicaginis infection among the three Medicago species investigated here, which can be used as a good candidate in crop rotation to reduce disease pressure in the field and as a source of P. medicaginis resistance for the improvement of forage legumes.

• 한국산림과학회지
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• 제89권5호
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• pp.666-676
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• 2000

굴참나무 천연집단의 형태적 변이(엽형 특성)를 연구하기 위하여 위도, 경도, 해발고 및 지리적 특성을 고려하여 19개 집단을 선발하였다. 각 집단별로 30개체를 대상으로 개체목당 수관 1/3 하단부에서 60개의 엽을 고르게 채집하여 엽신장, 최대엽폭, 엽맥수 및 엽병장을 조사한 후 그 값을 이용하여 엽신장/최대엽폭, 엽신장/엽병장, 상1/3폭/최대엽폭, 상1/3폭/하1/3폭 및 엽병장/엽맥수에 대한 형상비를 계산하여 엽형 특성에 대한 변이를 조사한 결과는 다음과 같다. 1. 엽신장/최대엽폭, 엽신장/엽병장, 엽병장/엽맥수, 상1/3폭/최대엽목, 상1/3폭/하1/3폭, 엽병장 및 엽맥수에 대한 변이는 집단간 및 집단내의 개체간에 고도의 유의적인 차이를 보였다. 특히, 분산기여도는 집단간보다 집단내의 개체간에 큰 것으로 나타나, 굴참나무의 연구는 집단보다 개체에 의한 연구가 바람직할 것으로 보여진다. 2. 엽형특성에 대한 주성분 분석은 첫 두 주성분까지의 적재 가여율이 80.5%로 굴참나무 엽형특성 분류에 중요한 정보를 주는 요인이었다. 각 인자별 기여도에 있어서는 제1주성분에서 엽병장/엽맥수 및 엽병장, 제2주성분에서 상1/3폭/최대엽폭, 상1/3폭/하1/3폭 및 엽맥수 인자가 높은 기여도를 나타냈다. 3. 위도, 경도 및 해발고에 따른 엽형특성에 대한 지리적 변이는 위도에 있어 엽신장/최대엽폭 및 엽신장/엽병장에 대해서는 정의 상관을 나타내었으며, 나머지 특성(엽병장/엽맥수, 상1/3폭/최대엽목, 상1/3폭/하1/3폭, 엽병장 및 엽맥수)들에 대해서는 부의 상관을 나타내었다. 그러나 경도와 해발고는 위도와 반대의 경향을 나타내었다. 4. 군집분석(Complete linkage cluster)한 결과는 거리지수 1.6에서 2개의 그룹 즉, I그룹(Pop. 1, 4, 5 및 13)과 II그룹(Pop. 2, 3, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18 및 19)으로 나뉘었으며, 거리지수 1.3에서는 II그룹이 다시 Pop. 3, 7, 10, 14, 15 및 17의 군집(II-1)과 Pop. 2, 6, 8, 9, 11, 12, 16, 18, 19의 군집(II-2)으로 나뒤어 총 3그룹으로 분리되었다. 대체적으로 이것은 사면의 방향에 대하여 I그룹은 북동면에, II-1그룹은 남동면에, 그리고 II-2그룹은 남서면에 집단이 위치하고 있어 환경조건이나 입지조건의 차이에 의하여 일어난 자연도태의 결과로 집단간 차이를 보이는 것으로 사료된다.

• 한국식물병리학회지
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• 제11권4호
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• pp.353-360
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• 1995

Tests were performed on 232 bacterial strains (71 strains of Pseudomonas tolaasii and 161 white line reacting organisms, WLRO) isolated from cultivated mushrooms. As results, P. tolaasii was divided into 5 groups on the basis of the phenotypical characteristics for the strains, and group 3 was the major one including 48 (62%) out of the total 71 strains. WLRO were classified into 23 groups, and group 10 was the major group (65 strains, 30% of the total WLRO tested). A white line was well formed at 22$^{\circ}C$ and at 4 mm distance between P. tolaasii and WLRO colonies in their dual culture on Pseudomonas agar F medium within 36-hr incubation, but not formed at $25^{\circ}C$ even for 72-hr incubation. The morphological, cultural and biological properties of P. tolaasii group 3, and the main group of WLRO, group 10, were different only in the light of pathogenicity. Also group 2 of P. tolaasii had the characteristics similar to group 24 of WLRO which was pathogenic to cultivated mushrooms, suggesting the P. tollaasiii and WLRO strains may be the same species although their white line forming ability and pathogenicity were more or less different from one another. Subculture of the strains in P. tolaasii group 1 induced the variation of their pathogenicity, white line forming ability and utilization of sodium benzoate and sodium tartrate, and thus it can be inferred that they were converted to strains having the characteristics of group 3 or WLRO groups.