The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Variability in Responses to Phoma medicaginis Infection in a Tunisian Collection of Three Annual Medicago Species

  • Mounawer Badri (Laboratory of Extremophile Plants, Centre of Biotechnology of Borj Cedria) ;
  • Amina Ayadi (Laboratory of Extremophile Plants, Centre of Biotechnology of Borj Cedria) ;
  • Asma Mahjoub (Laboratory of Extremophile Plants, Centre of Biotechnology of Borj Cedria) ;
  • Amani Benltoufa (Laboratory of Extremophile Plants, Centre of Biotechnology of Borj Cedria) ;
  • Manel Chaouachi (Laboratory of Bioactive Substances, Centre of Biotechnology of Borj Cedria) ;
  • Rania Ranouch (Laboratory of Bioactive Substances, Centre of Biotechnology of Borj Cedria) ;
  • Najah Ben Cheikh (Laboratory of Extremophile Plants, Centre of Biotechnology of Borj Cedria) ;
  • Aissa Abdelguerfi (Ecole Nationale Superieure Agronomique d'Alger) ;
  • Meriem Laouar (Ecole Nationale Superieure Agronomique d'Alger) ;
  • Chedly Abdelly (Laboratory of Extremophile Plants, Centre of Biotechnology of Borj Cedria) ;
  • Ndiko Ludidi (Plant Biotechnology Research Group, Department of Biotechnology, University of the Western Cape) ;
  • Naceur Djebali (Laboratory of Bioactive Substances, Centre of Biotechnology of Borj Cedria)
  • Received : 2022.09.19
  • Accepted : 2022.12.12
  • Published : 2023.04.01
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Abstract

Spring black stem and leaf spot, caused by Phoma medicaginis, is an issue in annual Medicago species. Therefore, in this study, we analyzed the response to P. medicaginis infection in a collection of 46 lines of three annual Medicago species (M. truncatula, M. ciliaris, and M. polymorpha) showing different geographic distribution in Tunisia. The reaction in the host to the disease is explained by the effects based on plant species, lines nested within species, treatment, the interaction of species × treatment, and the interaction of lines nested within species × treatment. Medicago ciliaris was the least affected for aerial growth under infection. Furthermore, the largest variation within species was found for M. truncatula under both conditions. Principal component analysis and hierarchical classification showed that M. ciliaris lines formed a separate group under control treatment and P. medicaginis infection and they are the most vigorous in growth. These results indicate that M. ciliaris is the least susceptible in response to P. medicaginis infection among the three Medicago species investigated here, which can be used as a good candidate in crop rotation to reduce disease pressure in the field and as a source of P. medicaginis resistance for the improvement of forage legumes.

Keywords

Acknowledgement

This research was funded by the Tunisian-Algerian bilateral project (2013-2015), and the Tunisian Ministry of Higher Education and Scientific Research (CBBC02 LR15) and the National Research Foundation of South Africa (GUN 120 114) in the framework of the Tunisian-South African Joint Research Collaboration Program (2019-2023).

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