• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.50-50
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• 2021

본 연구에서는 7년근과 13년근 산양삼을 선정하여 적변 유무에 따라 분류하고 적변에 따른 산양삼의 생육특성과 진세노사이드 함량을 조사하기 위해 수행되었다. 산양삼 재배지 9개소의 산양삼 지하부의 생육특성을 분석한 결과, 7년근과 13년근 산양삼의 생육특성은 적변에 따른 유의적인 차이는 확인되지 않았다. 적변에 따른 연근별 산양삼의 진세노사이드 함량을 비교 분석한 결과, 7년근 산양삼은 Rb1과 Rg1은 진세노사이드 함량이 일반삼에 비해 적변삼에서 높았고, 진세노사이드 Rc, Rd, Re, Rg2는 적변삼에 비해 일반삼에서 유의적으로 높은 것을 확인하였으나 적변에 따른 13년근 산양삼의 진세노사이드 함량 유의적인 차이는 확인할 수 없었다. 산양삼 생육특성과 진세노사이드 함량 간의 상관관계를 분석한 결과, 일반삼의 경우 진세노사이드 Rb2, Rc, Rd, Rf, Rg1이 산양삼의 지하부 길이와 유의적인 정의 상관관계를 보였고, 적변삼은 진세노사이드 Rd가 지하부 길이와는 유의적인 정의 상관관계, 주근직경과는 유의적인 부의 상관관계를 보였다. 본 연구에서는 7년근과 13년근 모두 적변에 따른 생육특성의 차이는 확인되지 않았고, 진세노사이드 함량은 7년근에서는 적변에 따라 유의적인 차이를 보였지만 13년근에서는 유의적인 차이가 없는 것을 확인하였다. 임가에서 산양삼은 7년근에 비해 13년근이 보다 고가에 거래되는 만큼 본 연구의 결과를 통해 적변삼에 대한 소비자들의 인식을 개선하여 산양삼 재배 농가의 소득 증대에 도움을 주고, 향후 산양삼의 품질규격을 정립하는 데 있어 유용한 정보를 제공할 수 있을 것으로 사료된다.

• Journal of Ginseng Research
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• 제44권6호
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• pp.815-822
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• 2020

Background: Recently, beneficial roles of ginsenoside F2 (GF2), a minor constituent of Panax ginseng, have been demonstrated in diverse inflammatory diseases. However, its roles in alcoholic liver inflammation and injury have not been clearly understood. Here, we investigated the underlying mechanism by which GF2 ameliorated alcoholic liver injury. Methods: To induce alcoholic liver injury, C57BL/6J wild type (WT) or interleukin (IL)-10 knockout (KO) mice were orally administered with ethanol (3 g/kg) or ethanol-containing GF2 (50 mg/kg) for 2 wk. Liver injury and infiltration of macrophages and neutrophils were evaluated by serum biochemistry and immunohistochemistry, respectively. The changes of hepatic immune cells were assessed by flow cytometry and polymerase chain reaction analysis. In vitro differentiation of naïve T cells was performed. Results: GF2 treatment significantly attenuated alcoholic liver injury, in which infiltrations of inflammatory macrophages and neutrophils were decreased. Moreover, the frequencies of Foxp3⁺ regulatory T cells (Tregs) increased but IL-17-producing T (Th17) cells decreased in GF2-treated mice compared to controls. Furthermore, the mRNA expression of IL-10 and Foxp3 was significantly increased, whereas IL-17 mRNA expression was suppressed in GF2-treated mice. However, these beneficial roles of GF2 were not observed in GF2-treated IL-10 KO mice, suggesting a critical role of IL-10. Similarly, GF2 treatment suppressed differentiation of naïve T cells into Th17 cells by inhibiting RORgt expression and stimulating Foxp3 expression. Conclusion: The present study suggests that GF2 treatment attenuates alcoholic liver injury by increasing IL-10 expression and Tregs and decreasing IL-17 expression and Th17 cells.

• 한국작물학회지
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• 제30권4호
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• pp.347-351
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• 1985

품종간 엽중수분변화에 대한 광합성과 호흡에 미치는 영향을 밝히기 위하여 온실에서 자란 잎담배 5품종의 엽중수분함량을 변화시켜서 광일광합성속도, 호흡속도, 기공저항 및 엽육저항을 각각 조사한 결과를 요약하면 다음과 같다. 1. 품종간 광합성속도는 N. longiflora가 20.7mg $CO_2$/dm$^2$/hr로 제일 컸고 Burley종이 제일 적었다. 2. 광합성속도와 단위엽면적당 건물중과는 정의 상관을 보였다. 3. 안동엽은 WSD 53%에서 광합성속도가 50%정도 감소되었으나 Burley 21은 외관상으로 위조상태 이전인 WSD 43%에서 광합성능력을 상실하여 재배품종은 N. longiflora와 안동엽보다 수분결핍에 의해 광합성 작용이 크게 억제되었다. 4. 품종간 호흡속도의 변화는 수분결핍 초기에 약간 증가하다가 광합성속도와 같은 경향으로 감소하였다. 5. 수분결핍에 따라 기공저항과 엽육저항은 증가되었으며 수분결핍에 의한 광합성속도의 감소는 주로 기공이 닫혀지기 때문으로 본다.

• The Plant Pathology Journal
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• 제25권1호
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• pp.77-85
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• 2009

Pseudomonas fluorescens strain Gpf01, isolated from ginseng rhizosphere showed antiviral activity against Cucumber mosaic virus, when tested in a local host of CMV, Chenopodium amaranticolor. Transposon mutant library of Gpf01 was prepared using pGS9::Tn5 and the mutant Gpf01-RS19 was found to loose antiviral production. We developed primers from the flanking region of Tn5 and found a cosmid clone pAV1123, harboring 1.2 kb antiviral compound producing (avcf01) locus. When a sub-clone pPH9, which carried 9.3 kb region of pAV1123, was introduced into antivirus deficient P. fluorescens wild type strain B16, it exhibited antiviral activity. Using Tn3-gus mutagenesis and complementation analysis, it was found that the genes related to antiviral activity production resided in a 9.3 kb HindIII-HindIII fragment of pAV1123, indicating that the plasmid carries an essential genes promoting antiviral activity.

• 대한암한의학회지
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• 제23권1호
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• pp.33-43
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• 2018

Objectives: To observe the mitigating effects of a Traditional Korean Medicine(TKM) treatment program especially including pharmacopuncture, with Cyclophosphamide and doxorubicin chemotherapy on a both sides breast cancer patient. Methods: AA 74 year-old female patient diagnosed with both sides breast cancer (Right) pT1bpN0M0, (Left) pT1cpN1Mx was admitted to hospital of Dong-eui university in May of 2017. She received Cyclophosphamide and Doxorubicin from May $31^{st}$ to August $2^{nd}$, 2017 followed by TKM treatment consisting of herbal medicine, acupuncture, moxibustion and pharmacopuncture (Trionycis Carapax, Non-toxic Bee Venom, and Cultivated Wild Ginseng Extract) for a period of almost 4 months, from May $13^{th}$ to August $19^{th}$, 2017. Symptoms were evaluated by the grade of chief complaints refer to Common Terminology Criteria for Adverse Events (CTCAE) and Eastern Cooperative Oncology Group (ECOG). Results: TKM including pharmacopuncture alleviated chemotherapy-induced nausea, fatigue, joint pain, diarrhea, insomnia. Conclusions: This case study potentiates TKM with pharmacopuncture's significant efficacy in aiding breast cancer patients suffering from Cyclophosphamide plus Doxorubicin induced adverse effects. Further research should take place for clear understanding of the exact amount of dosage and safety. Moreover it must be accompanied by long-term follow up researches.

• 대한한의진단학회지
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• 제16권2호
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• pp.47-58
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• 2012

Objectives : The purpose of the present study is to grasp and provide basic data necessary for the establishment of korean healthcare policies in future. Methods : A questionnaire developed by the author was distributed to 299 students of D University located in Busan and collected thereafter from March 5 to June 1, 2012. Statistical processing of the data obtained was conducted using the SPSS WIN IBM 20.0 program to indicate outcomes in frequencies and percentages. Results : The results of the survey are as follows. 1. The number of students who were mainly using korean hospitals/clinics was 6(2.0%). The most important factor in selecting korean medical institutions was 'medical technology'. 2. The treatment that was received the most frequently and the most reliable at korean medical institutions was acupuncture. The most frequent reason for taking korean medicine was 'prescriptions tailored to constitutions' and the most frequent reason for avoiding korean medicine was 'high prices'. 3. There was few number of students who knew that car insurance coverage for traffic accidents include treatment at korean clinics. and who knew about 'permitted standard herbal medicine', 'the use of disposable needles', 'wild ginseng pharmacopuncture'. 4. Among items necessary for the development of korean medicine, 'treatment technology with fast and high effects' was the most frequently selected. Conclusions : University students' preference for and perception of korean medicine were shown to be very low. It is considered that public relations should be reinforced to implant correct perception of korean medicine in university students who will be major users of hospitals and clinics.

• 동아시아식생활학회지
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• 제25권4호
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• pp.549-564
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• 2015

This study was conducted on the head family house of Panseogong coterie of Andong Kwon clans in Daksil village, Bongwha gun, Kyungbuk: historical sources survey and field visit over four rounds were conducted from March to June 2014. In-depth interview, storytelling, and nutrition facts were used as methods of research using 49 participants, and the potentials of trend goods were the main purpose of this research. The specialties of bongwha prefecture in the Chosun Dynasty were pine nut, manna lichen, ginseng, sweetfish, and wild honey. Ritual food bulcheonwi's offerings a feature in Choongjae Kwonbeol's head family - were sacred wine, me, gang, otang, myun, myunjeok, pyunjeok, pyunchung, pyun, po, chungpomook, dojeok, sookchae, ssam, chimchai, sikhye, chogwa, silgwa, soojunggwa, etc. The ritual foods of bulcheonwi's head family house Choongjae Kwonbeol were commercialized through storytelling. Choongjae Kwonbeol's ritual ceremonies, recognized for their national royalty, were reorganized as the traditional Korean table settings, so that the Korean people could easily access them. The special meal called "Geumgyeporansang" represented the head family house foods' essence to share and serve others through the Dacshil village's natural beauty, Bonghwa's local ingredients, and festivities after the "Chungjae Gwunbal Bulchun" rituals. The ritual foods called "Cheongamjungsang", utilizing "Chungjae Gwunbal Bulchun" rituals, were represented in the form of a lunch box: such foods illustrate the classical scholar's mindset that enjoys the nature and arts through education and virtue.

• 한국산학기술학회논문지
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• 제17권5호
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• pp.574-581
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• 2016

대 일본 단기소득임산물 수출액은 2010년 1,754만 달러에서 2014년 942만 달러로 2010년 대비 46.3% 감소하였다. 추가적인 노력을 통해 대 일본 수출 촉진이 필요한 시점이다. 본 연구는 일본의 FTA, TPP 등 시장 개방 여건 변화에 대응하여 밤, 표고버섯, 떫은감, 산양삼의 일본 시장에서의 경쟁력 확보 방안을 모색하기 위해 마련되었다. 본 연구에서는 일본 주부의 단기 임산물의 속성별 선호도를 분석하기 위하여 컨조인트 분석을 활용하였다. 분석 결과, 임산물별 속성 및 수준에 대한 뚜렷한 선호에 차이가 나타났으며, 한국산 단기소득임산물은 중국산 단기소득임산물 보다 선호되었으나 일본산 단기 소득임산물 보다는 경쟁력이 다소 떨어지는 것으로 나타났다. 또한 일본 주부는 가격에 민감하게 반응하지 않았으며, 다른 속성 변화에 더 민감하게 반응하는 것으로 나타났다. 본 연구에서 도출된 결과는 우리나라 주요 단기임산물의 대 일본 수출 방안 수립에 활용될 수 있을 것이다.

• 동의생리병리학회지
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• 제34권4호
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• pp.177-183
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• 2020

The Hippo-YAP signaling pathway is critical for cell proliferation, survival, and self-renewal in both Drosophila and mammals. Disorder of Hippo-YAP pathway leads to tumor development, progression and poor prognosis in various cancers. YAP/TAZ are the key downstream effectors of the Hippo pathway and they can be inhibited through LATS1/2, core kinases in the Hippo pathway, mediated phosphorylation. In this study, we investigated the effect of Angelica gigas Nakai extract (AGNE) on Hippo-YAP/TAZ pathway. First, ANGE induced YAP/TAZ phosphorylation and dissociation of the YAP/TAZ-TEAD transcription complex. By qRT-PCR, we found that ANGE inhibits the expression of YAP/TAZ-TEAD target gene, CTGF and CYR61. In addition, the transcriptional activity of YAP/TAZ was not suppressed significantly in LATS1/2 double-knockout (DKO) cells by ANGE compared to LATS1/2 wild-type (WT) cells, which means AGNE inhibits YAP/TAZ signaling through direct action on LATS1/2. Further, it was confirmed that AGNE-induced activation of LATS1/2 inhibited the migration potential of the vector-expressing cells by suppressing YAP/TAZ activity. The reduced migration potential was restored in active YAP-TEAD expressing cells. Taken together, the results of this study indicate that ANGE downregulates YAP/TAZ signaling in cells through the activation of LATS1/2.

• 대한한방내과학회지
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• 제42권3호
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• pp.444-454
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• 2021

Objectives: High natural killer cell activity (NKA) is associated with a clinically favorable status in patients with cancer. This study explores whether traditional Korean medicine (TKM) improves NKA in patients with cancer. Methods: We report three clinical cases involving patients with cancer who underwent NKA tests while they received TKM treatment, including acupuncture, moxibustion, wild ginseng pharmacopuncture, and Korean herbal medicines. We analyzed the treatment process and NKA test results of each patient. Results: The NKA of all three patients presented with an increasing tendency during the treatment process. Patient 1, who had been diagnosed with breast cancer, received inpatient treatment 3 times between September 16, 2017 and February 27, 2019. The NKA increased from 7.2 pg/mL to 571.7 pg/mL. Patient 2, who had thyroid cancer, was hospitalized 4 times between July 23, 2019 and August 27, 2020. The NKA improved from 317.4 pg/mL to 2000 pg/mL. Patient 3, who had liver cancer, received inpatient treatment 4 times between May 27, 2016 and January 10, 2017. The NKA increased from 22.2 pg/mL to 459.5 pg/mL. The chief complaints of these patients also were alleviated. Conclusions: TKM may be an effective treatment for accelerating NKA. Further research is needed to establish evidence that TKM stimulates NKA.