• Proceedings of the Korean Society of Crop Science Conference
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• 2022.04a
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• pp.98-98
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• 2022

• BMB Reports
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• v.32 no.1
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• pp.6-11
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• 1999

To understand the function of phospholipids and their fatty acid composition on the morphological changes in the amp 1-4 mutant of Arabidopsis, the mutant was compared to the wild-type Arabidopsis by TLC, HPTLC, phosphorous assay, HPLC, and GC. In the mutant, phosphatidylethanolamine (PE) was increased 5-fold and phosphatidylglycerol (PG) was decreased 1.2-fold (nmol phosphorous/g tissue). Inositol phospholipids showed a generally increased trend ranging from 1.4-to 3.0-fold (nmol inositol/g tissue). When fatty acid composition of the mutant was compared to the wild-type, linoleic (18:2) and linolenic (18:3) acids of phosphatidylcholine (PC) and PG were decreased but palmitoleic acid (16:1) and oleic acid (18:1) of PC was increased 2.5- and 2.1-fold (mol%), respectively. In galactolipids, myristic acid (14:0) of monogalactosyl-diacylglycerol (MGDG) were increased 5.8-fold (mol%). Among the inositol phospholipids, lysophosphatidylinositol (L-PI) and phosphatidylinositol 4,5-bisphosphate ($PIP_2$) showed 4-and 1.9-fold (mol%) increase of 16:1, respectively. These results suggest that the increase of PE, the decrease of PG, the increase of inositol phospholipids, and the altered fatty acid composition are related to the phenotypic changes affecting the morphological features, and might cause different physiological changes in the amp 1-4 mutant compared to wild-type Arabidopsis.

• Korean Journal of Plant Resources
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• v.36 no.1
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• pp.26-31
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• 2023

In this study, we investigated in vitro inhibitory activity of wild-simulated ginseng (WSG) against non-alcoholic fatty liver disease using HepG-2 cells. T0901317 treatment increased the lipid accumulation in HepG-2 cells, but WSG treatment inhibited T0901317-mediated lipid accumulation. In addition, WSG downregulated T0901317-mediated expression of SREBP-1c, ACC, FAS and SCD-1 protein. In addition, WSG increased the phosphorylation level of LKB1 and AMPK. Compound C treatment blocked WSG-mediated downregulation of SREBP-1c protein. In conclusion, WSG is considered to inhibit the accumulation of lipids and triglycerides in HepG-2 cells by inducing the activation of LKB1 and AMPK successively, thereby reducing the expression of FAS, ACC, and SCD-1 through suppression of SREBP-1c expression.

• Interdisciplinary Bio Central
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• v.2 no.4
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• pp.13.1-13.8
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• 2010

The National BioResource Project of Japan is a governmental project to promote domestic/international research activities using biological resources. The project has 27 biological resources including three cereal resources. The core center and sub-center which historically collected the cereal resources were selected for each cereal program. These resources are categorized into several different types in the project; germplasm, genetic stocks, genome resources and database information. Contents of rice resources are wild species, local varieties in East and Southwest Asia & wild relatives, MNU-induced chemical mutant lines, marker tester lines, chromosome substitution lines and other experimental lines. Contents of wheat resources are wild strains, cultivated strains, experimental lines, rye wild and cultivated strains; EST clones and full-length cDNA clones. Contents of barley resources are cultivar and experimental lines, core collection, EST/cDNA clones, BAC clones, their filters and superpool DNA. Each resource is accessible from the online database to see the contents and information about the resources. Links to the genome information and genomic tools are also important function of each database. The major contents and some examples are presented here.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.215-215
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• 2022

Starch synthase proteins (SSI, SSII and SSIII) in rice are mainly involved in amylopectin synthesis mediating its chain elongation, and the functional loss of SSII can increase amylose accumulation through decreasing of amylopectin chain proportions. For purposes of identifying functional haplotypes and evolutionary analyses of this gene, SSII-1, we investigated 374 rice accessions belonging to different subgroups of origins. We subsequently performed bioinformatic analyses on their variations through haplotyping, resequencing and structuring based on different classified populations. Haplotyping of cultivated rice accessions using genetic variations within SSII-1 genomic region of chromosome 10 revealed a total of 8 haplotypes, representing 6 functional haplotypes by 4 non-synonymous SNPs of three different exons (1, 4 and 10), which effect on protein structure. Higher nucleotide diversity value was found in wild group (0.0055) compared to any of cultivated subpopulations, of which aus showed the most reduction of diversity value (0.0003). Tajima's D analysis exhibits the most Tajima's D value only in admixture group (0.3600) which appears to be the cause of a sudden population contraction by rare alleles scarcity. A clear separation of some wild accessions from the admixed cultivated subpopulations was observed in PCA and phylogenetic analysis. Similar admixed pattern of population structure was estimated with an increased K values of 2 to 8 where genetic components of almost all cultivated subpopulations were shared with the wild which can also be subsequently estimated by very low F_ST-values by -0.011 (wild-aromatic) and -0.003 (wild-admixture).

• Weed & Turfgrass Science
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• v.5 no.4
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• pp.196-202
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• 2016

With increasing LM soybean import, the concern about unintentional gene flow from LM soybean to wild soybean and consequential weedy risk has been growing. Therefore, we conducted this study to characterize seed traits including germination of hybrids resulted from gene flow from LM soybean to wild soybean in comparison with their parents, LM soybean and wild soybean. Pollen-donor LM soybean seeds were much greater and heavier (about 15.0 g of 100 seed weight) than F2 hybrid (5.7 g), while pollen-recipient wild soybean and F1 hybrid seeds were smallest and lightest (about 2.5 g). F2 hybrid was brown, intermediate between yellow LM soybean seed and black wild soybean seed. These findings indicate that F1 hybrid seeds show similar characteristics with wild soybean, while F2 hybrid seeds show intermediate color and size between two parents. F2 hybrid seed showed intermediate traits between two parents in germination and dormancy rates, which were 35% and 65%, respectively. LM soybean showed no dormancy, while wild soybean showed greater than 90% dormancy. This finding indicates that F2 hybrid show intermediate characteristics in seed germination with high dormancy trait, suggesting a potential weediness of hybrids resulted from gene flow from LM soybean to wild soybean.

• The Korea Journal of Herbology
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• v.38 no.5
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• pp.85-95
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• 2023

Objectives : This study was intended to reveal the chemical profiles of aerial(leaf, stem) and underground(rhizome, radix) parts of wild ginseng, and to investigate their anti-aging effects on human skin cells. Methods : Wild ginseng, estimated for over 20 years, was divided into the aerial and underground parts. Total phenolic contents of each extracts were measured using a Folin-ciocalteu method. The contents of 18 amino acids, 8 minerals and 27 ginsenosides were determined by GC-FID, ICP-MS and LC-MS, respectively. The anti-aging effects, including the radical scavenging activity, the activation of mitochondrial function on human fibroblasts, and the proliferation activity on human keratinocyte progenitor cells, for the whole plant and underground part of wild ginseng were evaluated. Results : The total phenolic acids, amino acids, and minerals in the aerial part were more than twice as high as in the underground part. Compared to the cultivated ginseng root, there were various types of ginsenosides in both parts of wild ginseng, and the total amount was more than twice as high. In particular, the aerial part significantly contained ginsenoside F1, F2, C-Mc1, and C-O, and the distinctive patterns that distinguish each parts of wild ginseng from the cultivated ginseng root were derived. The whole plant and underground part of wild ginseng exhibited significant antioxidant effect(14.3-45.6%), activation of mitochondrial membrane potential(105.5-120.1%), and cell proliferation(112.1-125.4%). Conclusions : The entire plant and underground part of wild ginseng are high value-added plants and have beneficial effects on skin anti-aging properties through its abundant metabolites.

• Korean Journal of Plant Resources
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• v.21 no.1
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• pp.41-46
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• 2008

Molecular relationship and genetic diversity of 21 wild tea collections which grown natural region in Korea were investigated based on PCR-RFLP analysis using DFR genes. Approximately 1.4kb fragment of the DFR gene from wild tea samples were successfully amplified use DFR 4+5 primer pair. On the bases of restriction fragment length polymorphism(RFLP) analysis using Hpa II and Mse I enzymes, three different band patterns shown from Hpa II enzyme and showed genetic diversity between same region wild tea group. Six kind of restriction enzyme profiles obtained from digested with restriction endonuclease Mse I and shown two kind of restriction enzyme profiles collected from same region wild tea at Ungpo. The results of RFLP analysis indicated that wild tea showed genetic diversity among different regions of tea groups, but also between same region wild tea.

• Journal of Ecology and Environment
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• v.44 no.3
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• pp.143-154
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• 2020

Background: Korean wild boars (Sus scrofa coreanus Heude), because of their adaptability, are a widespread large mammal; however, they sometimes cause problems by invading farms and eating the crops, creating insufficiencies of some foods in South Korea. To understand the diet composition of Korean wild boars according to sex and body size, we collected their feces from Mt. Jeombongsan, Seoraksan National Park, South Korea. The sizes of fecal samples were measured, and genomic DNA was extracted from the samples. We amplified specific loci targeting plants (rbcL and trnL) and animals (COI) to detect the food sources of this omnivore and amplified the ZF and SRY regions to determine the sex. Results: In the wild boar feces, Rosaceae and Bryophyte were the most frequently detected plant food sources at the family level and Diptera and Haplotaxida were the most frequently detected animal food sources at the order level. As a result of sex determination, the sex ratio of wild boars collected in the Mt. Jeombongsan area was approximately 1:1. Our result suggested that there is no significant difference between the diet composition of male and female boars. Based on the average cross-sectional area of the feces, the top 25% were classified into the large body size group and the bottom 25% were classified into the small body size group. The large body size group mainly preferred Actinidiaceae, and the small body size group most frequently consumed Fagaceae. The diet of the large body size group was more diverse than the small body size group. Conclusions: Our results showed that the wild boars preferred Rosaceae, especially Sanguisorba and Filipendula, as plant food sources, and Diptera and Coleoptera of Insecta as animal food sources. Based on the results, the dietary preferences of wild boar appear to be distinguished by not their sex but their body size. Our study could help to elucidate the feeding ecology and population structure of wild boar, as well as address conservation and management issues.

• Journal of Ecology and Environment
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• v.36 no.3
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• pp.167-172
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• 2013

A Two-year field experiment was carried out in 2009 and 2010 to evaluate the effects of different densities of wild oat and nitrogen (N) rates on oilseed rape yield and yield components. Experimental design was split plot with three replications. Increased nitrogen rates up to 150 kg N/ha caused an increase in oilseed rape grain yield in weed-free plots (4.26 t/ha; 1t = $10^3kg$), while even at the lowest density of wild oat (15 $plants/m^2$), increasing N fertilizer only up to 50 kg/ha led to a significant increase in grain yield. Wild oat growth was favored by high levels of N. Intraspecific competition reduced biomass, 100-seed weight and number of tillers of wild oat. Both lower N rate and intraspecific competition had negative effect on wild oat growth but the weed was still able to produce a considerable number of tillers and vigorous seeds. It showed that wild oat possess a notable adaptive plasticity and can allocate a greater proportion of its resource toward reproductive organs even under resource shortage conditions and, thus, it may increase the competitive ability of the weed over the crop.Our results suggest that effective weed management should be done to prevent wild oat seed production in crops preceding oilseed rape as well as to control the weed at early season in oilseed rape fields.