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Genetic Diversity of Wild Tea(Camellia sinensis L.) in Korea  

Oh, Chan-Jin (Jeonnam Forest Environment Research Institute)
Lee, Sol (Division of Forest Science, College of Agriculture & Life Science, Chonbuk National University)
You, Han-Choon (Jeonnam Forest Environment Research Institute)
Chae, Jeong-Gi (Division of Forest Resources and Landscape Architecture, College of Agriculture & Life Science, Jeonnam National University)
Han, Sang-Sub (Division of Forest Science, College of Agriculture & Life Science, Chonbuk National University)
Publication Information
Korean Journal of Plant Resources / v.21, no.1, 2008 , pp. 41-46 More about this Journal
Abstract
Molecular relationship and genetic diversity of 21 wild tea collections which grown natural region in Korea were investigated based on PCR-RFLP analysis using DFR genes. Approximately 1.4kb fragment of the DFR gene from wild tea samples were successfully amplified use DFR 4+5 primer pair. On the bases of restriction fragment length polymorphism(RFLP) analysis using Hpa II and Mse I enzymes, three different band patterns shown from Hpa II enzyme and showed genetic diversity between same region wild tea group. Six kind of restriction enzyme profiles obtained from digested with restriction endonuclease Mse I and shown two kind of restriction enzyme profiles collected from same region wild tea at Ungpo. The results of RFLP analysis indicated that wild tea showed genetic diversity among different regions of tea groups, but also between same region wild tea.
Keywords
Wild Tea(Camellia sinensis L.); PCR-RFLP; DFR;
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