• Journal of Microbiology and Biotechnology
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• v.24 no.5
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• pp.619-628
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• 2014

To identify lipase LipA (PFL_0617) from Pseudomonas protegens Pf-5, a lipA deletion mutant (Pf0617) and a complementary strain (Pf0617lipA) were constructed, and their effects on the lipase production were examined. Pf0617 remarkably decreased its whole-cell lipase activity, whereas Pf0617lipA made its whole-cell lipase activity not only restore to wild-type level but also get a further increment. However, the deletion and overexpression of lipA did not affect the extracellular lipase activity. In addition, the unbroken whole cells of these strains were able to catalyze the hydrolysis of membrane-permeable p-nitrophenyl esters, but could not hydrolyze the membrane-impermeable olive oil. These results confirmed that LipA was an intracellular lipase and Pf-5 could also be used as a natural whole-cell biocatalyst. To evaluate the potential of Pf-5 as a whole-cell biocatalyst and separately characterize the whole-cell LipA, the properties of the whole-cell lipases from Pf-5 and Top10lipA were characterized. The results demonstrated that both Pf-5 and Top10lipA exhibited high tolerance to alkaline condition, high temperature, heavy metal ions, surfactants, and organic solvents. Taken together, lipA can realize functional expression in E. coli Top10, and Pf-5 and Top10lipA as whole-cell biocatalysts may have enormous potential in applications.

• Journal of Microbiology and Biotechnology
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• v.27 no.1
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• pp.92-100
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• 2017

Acetoin (AC) is a volatile platform compound with various potential industrial applications. AC contains two stereoisomeric forms: (3S)-AC and (3R)-AC. Optically pure AC is an important potential intermediate and widely used as a precursor to synthesize novel optically active materials. In this study, chiral (3R)-AC production from meso-2,3-butanediol (meso-2,3-BD) was obtained using recombinant Escherichia coli cells co-expressing meso-2,3-butanediol dehydrogenase (meso-2,3-BDH), NADH oxidase (NOX), and hemoglobin protein (VHB) from Serratia sp. T241, Lactobacillus brevis, and Vitreoscilla, respectively. The new biocatalyst of E. coli/pET-mbdh-nox-vgb was developed and the bioconversion conditions were optimized. Under the optimal conditions, 86.74 g/l of (3R)-AC with the productivity of 3.61 g/l/h and the stereoisomeric purity of 97.89% was achieved from 93.73 g/l meso-2,3-BD using the whole-cell biocatalyst. The yield and productivity were new records for (3R)-AC production. The results exhibit the industrial potential for (3R)-AC production via whole-cell biocatalysis.

• Journal of The Korean Association For Science Education
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• v.8 no.2
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• pp.53-56
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• 1988

The Korean Federation of Education Association has published "Vacation Life" to promote self-teaching of middle school students during the summer and winter vacation of middle school students every year. The book include whole subjecy offered in the middle school. However subject of science(Physics, chemistry, biology and earth science) is more emphasized. This article described brief summary of the contents of biology branch published in winter "Vacation Life", 1980 and summer "Vacation Life" 1981.

• East Asian mathematical journal
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• v.31 no.2
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• pp.189-210
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• 2015

Character means a person's nature and the mind and behavior to lead a desirable life personally and socially. Also character can be changed with education and environment. Therefore character education will be possible to do practice through various educational activities and life experiences in school. In other words, we think that students will get person's character required to lead a desirable life personally and socially beyond what they just learn knowledge and skill, enter the higher schools and succeed one's way. This study accepts merits of existing character education policy through subject(mathematics) education and focus on direction for character education through subject(mathematics) education for systematic and synthetic approach for practice of character education. For this, we will see applicability of the plan for character education in the whole subject education, in particular focusing on cases of character education in mathematics subject.

• Journal of Korean Home Economics Education Association
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• v.16 no.1
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• pp.31-45
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• 2004

The purposes of this study are to explore the several aspects associated with school life long education through home and family life education by home economics teachers and suggest the research and practicing model for this education and ways of activating it. The home and family life education with life long education perspective is defined and discussed why this education is needed in today's rapid changing society. The characteristics of school life long education are explained in terms of definition. legal basis, purposes and roles The needs of school life long education through home and family life education by home economics teachers are discussed in such ways as enhancing the public relations and the image of home economics education, contributing of home economics teachers to community and achieving the identity of home economics as a subject matter in secondary school. The systems model suggested for studying and practicing school life long education through home and family life education is consisted of input. throughput, and output. The element including in each process is related with each other thus the change of one element affects on both the other elements and the whole system. Several ways of activating of this education are recommended.

• Journal of the Korean Home Economics Association
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• v.34 no.3
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• pp.327-343
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• 1996

This study is the last part of a collaborative study on the Family Life Issues of Middle aged Apartment-Residents in Seoul. This study is focused on case studies. The data were collected from 15 fulltime housewives who are in the upper-middle and the lower-middle families having at least one child in a middle or high school from Kangbuk and Kangnam areas. In this study, the whole family life issues were divided into the following 4 subareas ; 1. housewives' own life, 2. family life, 3. economic environments, 4. housing environments. The major result of this study is : the most important factor influencing the quality of life of the middle age family was the existence of children attending junior and senior high school, which brought various problems into the family with relation to self identity, time management and leisure of middle aged housewife, educational cost, shared family activities, housing space. Therefore the whole family life would be improved through the change of the current education system.

• Hwankyungkyoyuk
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• v.12 no.1
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• pp.64-75
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• 1999

The goals of moral education according to the 7th educational curriculum are (1) to learn the basic life custom and ethical norms necessary to desirable life, (2) to develop the judgment to solve desirably and practically the ethical matters in daily life, (3) to develop the sound citizenship, national identity and consciousness, and the consciousness of world peace and mankind's mutual prosperity, and (4) to develop the ethical propensity to practice the ideal and principle of life systematically Based on the goals in the above, the following can be established as goals of environmental education possible: (1) to learn judgment to solve practically the environmental problems in the society with their ethical understanding, and (2) to recognize that environmental consciousness is the basic necessity of sound citizenship and national identity and consciousness, and mankind's mutual prosperity, and to have attitudes to practice environmental preservation in daily life. Like these, the intellectual aspect, the affective aspect, and the active aspect can be established in the environmental education in the ethics education keeping their balance. In order to achieve its goals, the contents of ethics subject are organized largely with 4 domains: (1) individual life, (2) home life, life with neighbors, and school life, (3) social life, and (4) national life. Among these, environmental education is mainly included in the domain of social life. These contents concerning environmental education take 22 (32.4%) out of the whole 68 teaching factors which are taught in the ethics subject from the 3rd grade to 10th grade. These 22 environmental teaching factors are mainly related to environmental ethics, environmental preservation and measures, and sound consumption life. Classified according to each goal, the environmental contents in the 7th curriculum for ethics subject put emphasis on environmental value and attitudes, action and participation, and information and knowledge. Therefore, the recommendable teaching and learning method for the environmental education in ethics subject is to motivate students' practice or to make them practice in person. For example, role-play model, value-conflict model, group study model can be applied according to the topics of environmental education.

• International Journal of Reliability and Applications
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• v.5 no.4
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• pp.155-162
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• 2004

A new class of life distributions is studied. This class is defined based on comparing the residual life to the whole life in the moment generating function order giving "the new better than used in the moment generating function order ageing class ($NBU_{mgf}$)". Some new results of this class are given including some closere properties and characterizations. Finally testing exponentially against the $NBU_{mgf}$ class is also addressed.

• International Journal of Computer Science & Network Security
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• v.22 no.12
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• pp.146-152
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• 2022

The National Agency for Quality Assurance in Higher Education plays a crucial role in education in Ukraine, as an independent entity creates and ensures quality standards of higher education, which allow to properly implement the educational policy of the state, develop the economy and society as a whole. The purpose of the article: to reveal the crucial role of the National Agency for Quality Assurance in Higher Education to create quality management of higher education institutions, to show its mechanism as an independent entity that creates and ensures quality standards of higher education. and society as a whole. The mission of the National Agency for Quality Assurance in Higher Education is to become a catalyst for positive changes in higher education and the formation of a culture of its quality. The strategic goals of the National Agency are implemented in three main areas: the quality of educational services, recognition of the quality of scientific results, ensuring the systemic impact of the National Agency. The National Agency for Quality Assurance in Higher Education exercises various powers, which can be divided into: regulatory, analytical, accreditation, control, communication. The effectiveness of the work of the National Agency for Quality Assurance in Higher Education for 2020 has been proved. The results of a survey conducted by 183 higher education institutions of Ukraine conducted by the National Agency for Quality Assurance in Higher Education are shown. Emphasis was placed on the development of "Recommendations of the National Agency for Quality Assurance in Higher Education regarding the introduction of an internal quality assurance system." The international activity and international recognition of the National Agency for Quality Assurance in Higher Education are shown.

• Journal for History of Mathematics
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• v.22 no.3
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• pp.153-170
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• 2009

Zoltan, P. Dienes is a famous researcher and practitioner who has tried to teach mathematical structures to children for about 50 years. Even though his ideas of teaching mathematics and materials including MAB have been well known in Korea, they are only a part of his achievement he has developed for his whole life. So this article is designed for taking an overview of his whole life and achievement and getting some implications for today's mathematics education. In this article, his life story could be divided by five periods in terms of a scholastic career and his research achievement could be reorganized with respect to five theses: psychology of learning mathematics, mathematical curriculum, teacher education, games and material for mathematical learning. As a result, it is found that there is a deep connection between his personal life and his scholastic career.