• Journal of Microbiology and Biotechnology
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• 제24권5호
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• pp.619-628
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• 2014

To identify lipase LipA (PFL_0617) from Pseudomonas protegens Pf-5, a lipA deletion mutant (Pf0617) and a complementary strain (Pf0617lipA) were constructed, and their effects on the lipase production were examined. Pf0617 remarkably decreased its whole-cell lipase activity, whereas Pf0617lipA made its whole-cell lipase activity not only restore to wild-type level but also get a further increment. However, the deletion and overexpression of lipA did not affect the extracellular lipase activity. In addition, the unbroken whole cells of these strains were able to catalyze the hydrolysis of membrane-permeable p-nitrophenyl esters, but could not hydrolyze the membrane-impermeable olive oil. These results confirmed that LipA was an intracellular lipase and Pf-5 could also be used as a natural whole-cell biocatalyst. To evaluate the potential of Pf-5 as a whole-cell biocatalyst and separately characterize the whole-cell LipA, the properties of the whole-cell lipases from Pf-5 and Top10lipA were characterized. The results demonstrated that both Pf-5 and Top10lipA exhibited high tolerance to alkaline condition, high temperature, heavy metal ions, surfactants, and organic solvents. Taken together, lipA can realize functional expression in E. coli Top10, and Pf-5 and Top10lipA as whole-cell biocatalysts may have enormous potential in applications.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.92-100
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• 2017

Acetoin (AC) is a volatile platform compound with various potential industrial applications. AC contains two stereoisomeric forms: (3S)-AC and (3R)-AC. Optically pure AC is an important potential intermediate and widely used as a precursor to synthesize novel optically active materials. In this study, chiral (3R)-AC production from meso-2,3-butanediol (meso-2,3-BD) was obtained using recombinant Escherichia coli cells co-expressing meso-2,3-butanediol dehydrogenase (meso-2,3-BDH), NADH oxidase (NOX), and hemoglobin protein (VHB) from Serratia sp. T241, Lactobacillus brevis, and Vitreoscilla, respectively. The new biocatalyst of E. coli/pET-mbdh-nox-vgb was developed and the bioconversion conditions were optimized. Under the optimal conditions, 86.74 g/l of (3R)-AC with the productivity of 3.61 g/l/h and the stereoisomeric purity of 97.89% was achieved from 93.73 g/l meso-2,3-BD using the whole-cell biocatalyst. The yield and productivity were new records for (3R)-AC production. The results exhibit the industrial potential for (3R)-AC production via whole-cell biocatalysis.

• 한국과학교육학회지
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• 제8권2호
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• pp.53-56
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• 1988

The Korean Federation of Education Association has published "Vacation Life" to promote self-teaching of middle school students during the summer and winter vacation of middle school students every year. The book include whole subjecy offered in the middle school. However subject of science(Physics, chemistry, biology and earth science) is more emphasized. This article described brief summary of the contents of biology branch published in winter "Vacation Life", 1980 and summer "Vacation Life" 1981.

• East Asian mathematical journal
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• 제31권2호
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• pp.189-210
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• 2015

Character means a person's nature and the mind and behavior to lead a desirable life personally and socially. Also character can be changed with education and environment. Therefore character education will be possible to do practice through various educational activities and life experiences in school. In other words, we think that students will get person's character required to lead a desirable life personally and socially beyond what they just learn knowledge and skill, enter the higher schools and succeed one's way. This study accepts merits of existing character education policy through subject(mathematics) education and focus on direction for character education through subject(mathematics) education for systematic and synthetic approach for practice of character education. For this, we will see applicability of the plan for character education in the whole subject education, in particular focusing on cases of character education in mathematics subject.

• 한국가정과교육학회지
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• 제16권1호
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• pp.31-45
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• 2004

본 연구는 학교 평생교육 관점에서의 가정생활 교육과 관련된 제반 양상을 다각적으로 분석하여 체계적 접근법을 통하여 모형화 시키고 이를 기초로 활성화 방안을 모색해 본 탐색적 연구이다. 관련문헌과 선행연구 고찰을 통하여 분석한 결과 변화하는 사회에서 평생교육과 가정생활교육의 요구는 지속적으로 증가될 것으로 나타났고, 학교는 학교가 가진 평생교육기관으로서의 제반 특성 때문에 평생교육의 구심체적 기능을 수행할 수 있는 것으로 나타났다. 또한 학교 평생 교육적 관점에서의 가정생활 교육은 교육 수요자의 교육요구 측면이나. 교과 이미지와 정체성 강화 및 전공자의 사회적 기여를 증진시키는 측면에서 필요하다고 나타났다. 마지막으로 학교 평생 교육적 관점에서의 가정생활 교육의 연구와 현장 실천을 위하여 탐색적 체계모형이 제안되었고. 이 모형에 근거한 활성화 방안이 모색되었다.

• 대한가정학회지
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• 제34권3호
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• pp.327-343
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• 1996

This study is the last part of a collaborative study on the Family Life Issues of Middle aged Apartment-Residents in Seoul. This study is focused on case studies. The data were collected from 15 fulltime housewives who are in the upper-middle and the lower-middle families having at least one child in a middle or high school from Kangbuk and Kangnam areas. In this study, the whole family life issues were divided into the following 4 subareas ; 1. housewives' own life, 2. family life, 3. economic environments, 4. housing environments. The major result of this study is : the most important factor influencing the quality of life of the middle age family was the existence of children attending junior and senior high school, which brought various problems into the family with relation to self identity, time management and leisure of middle aged housewife, educational cost, shared family activities, housing space. Therefore the whole family life would be improved through the change of the current education system.

• 한국환경교육학회지:환경교육
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• 제12권1호
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• pp.64-75
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• 1999

The goals of moral education according to the 7th educational curriculum are (1) to learn the basic life custom and ethical norms necessary to desirable life, (2) to develop the judgment to solve desirably and practically the ethical matters in daily life, (3) to develop the sound citizenship, national identity and consciousness, and the consciousness of world peace and mankind's mutual prosperity, and (4) to develop the ethical propensity to practice the ideal and principle of life systematically Based on the goals in the above, the following can be established as goals of environmental education possible: (1) to learn judgment to solve practically the environmental problems in the society with their ethical understanding, and (2) to recognize that environmental consciousness is the basic necessity of sound citizenship and national identity and consciousness, and mankind's mutual prosperity, and to have attitudes to practice environmental preservation in daily life. Like these, the intellectual aspect, the affective aspect, and the active aspect can be established in the environmental education in the ethics education keeping their balance. In order to achieve its goals, the contents of ethics subject are organized largely with 4 domains: (1) individual life, (2) home life, life with neighbors, and school life, (3) social life, and (4) national life. Among these, environmental education is mainly included in the domain of social life. These contents concerning environmental education take 22 (32.4%) out of the whole 68 teaching factors which are taught in the ethics subject from the 3rd grade to 10th grade. These 22 environmental teaching factors are mainly related to environmental ethics, environmental preservation and measures, and sound consumption life. Classified according to each goal, the environmental contents in the 7th curriculum for ethics subject put emphasis on environmental value and attitudes, action and participation, and information and knowledge. Therefore, the recommendable teaching and learning method for the environmental education in ethics subject is to motivate students' practice or to make them practice in person. For example, role-play model, value-conflict model, group study model can be applied according to the topics of environmental education.

• International Journal of Reliability and Applications
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• 제5권4호
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• pp.155-162
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• 2004

A new class of life distributions is studied. This class is defined based on comparing the residual life to the whole life in the moment generating function order giving "the new better than used in the moment generating function order ageing class ($NBU_{mgf}$)". Some new results of this class are given including some closere properties and characterizations. Finally testing exponentially against the $NBU_{mgf}$ class is also addressed.

• International Journal of Computer Science & Network Security
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• 제22권12호
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• pp.146-152
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• 2022

The National Agency for Quality Assurance in Higher Education plays a crucial role in education in Ukraine, as an independent entity creates and ensures quality standards of higher education, which allow to properly implement the educational policy of the state, develop the economy and society as a whole. The purpose of the article: to reveal the crucial role of the National Agency for Quality Assurance in Higher Education to create quality management of higher education institutions, to show its mechanism as an independent entity that creates and ensures quality standards of higher education. and society as a whole. The mission of the National Agency for Quality Assurance in Higher Education is to become a catalyst for positive changes in higher education and the formation of a culture of its quality. The strategic goals of the National Agency are implemented in three main areas: the quality of educational services, recognition of the quality of scientific results, ensuring the systemic impact of the National Agency. The National Agency for Quality Assurance in Higher Education exercises various powers, which can be divided into: regulatory, analytical, accreditation, control, communication. The effectiveness of the work of the National Agency for Quality Assurance in Higher Education for 2020 has been proved. The results of a survey conducted by 183 higher education institutions of Ukraine conducted by the National Agency for Quality Assurance in Higher Education are shown. Emphasis was placed on the development of "Recommendations of the National Agency for Quality Assurance in Higher Education regarding the introduction of an internal quality assurance system." The international activity and international recognition of the National Agency for Quality Assurance in Higher Education are shown.

• 한국수학사학회지
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• 제22권3호
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• pp.153-170
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• 2009

Dienes는 새수학 운동가로서, 수학의 구조를 어린 학생들에게 가르치고자 부단히 노력한 연구가이며, 실천가이다. 국내에서는 그의 수학교육 이론이 부분적으로 알려져 있지만, 그것은 50여 년 간 Dienes가 남긴 연구 업적의 일부에 불과하다. 이 연구에서는 약 90여 년 간의 그의 긴 일생과 약 50 여 년간의 연구 일생을 돌아보고, 그것이 오늘날의 수학교육에 주는 의미를 되새겨 보고자 한다. 먼저 그의 일생은 그의 회고록과 기타 연구 논문에 언급된 내용을 바탕으로 크게 다섯 부분으로 구분하여 정리하였다. 그의 연구 업적은 Dienes가 연구 전성기에 관심을 가졌던 분야로 꼽은 수학학습심리 이론, 교육과정개발, 교사교육, 그가 개발한 교구와 게임등의 주제를 바탕으로 정리하였다. 이러한 고찰을 통해 그의 학문적 성향과 연구 내용이 그의 인간적 삶의 배경과 역사와 무관하지 않음을 알 수 있었다.