• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.33 no.2
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• pp.70-82
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• 2020

Objectives : Recently the aesthetic, especially skin whitening, is focused on. This study was designed to investigate the effects of Gypsum Extract(GE) on skin whitening using B16F10 cell lines. Methods : In this study, we investigated effect of GE on cell viability, melanin synthesis, Superoxide dismutase(SOD)-like activity and tyrosinase activity in vitro and in vivo. Results : In results, The proliferation of B16F10 cell lines was increased by GE concentration. But there was no statistical signification. Melanin synthesis was decreased except GE 125㎍/㎖ of treated group. And the melanin synthesis ratio was increased in group being treated α-MSH. But After treating GE, it was decreased compared to Non-GE treated group. In addition, the SOD-like activity was enhanced in more than GE 125㎍/㎖ of treated group. And the activity of tyrosinase in vitro was inhibited in GE 500㎍/㎖ and 1,000㎍/㎖ of treated groups. The activity of tyrosinase in vivo was reduced in both Arbutin 500㎍/㎖ of treated group and Gypsum 500㎍/㎖ of treated group compared to controled group. After being treated α-MSH, the activity of tyrosinase was enhanced. But after treating Arbutin 500㎍/㎖ or Gypsum 500㎍/㎖, it was inhibited being compared to the group of Non-medicament treated. Conclusion : The results of this study showed the effects of GE that can inhibit melanin synthesis and the activity of tyrosinase and enhance the SOD-like activity. It means that gypsum has the skin whitening effect.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.30 no.4
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• pp.49-61
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• 2017

Objectives : In order to find out the whitening effects of Leonuri Herba, this study was designed to identify the effects and the action mechanism of LHE(Leonuri Herba extract) on Malignant melanoma cell lines. Methods : After treating LHE on the B16F10 cell-Malignant melanoma cell line-, the cell survival rate, melanin biosynthesis rate, intra&extracellular tyrosinase activity rate, SOD-like activity, tyrosinase mRNA gene expression were investigated. The results were compared with control group without LHE treatment or with positive control group treated with whitening efficacy substance. Results : B16F10 cell survival rate, melanin biosynthesis rate, and intra&extracellular tyrosinase activity were significantly inhibited depending on the concentration of treated LHE. Melanin biosynthesis rate and tyrosinase activity rate were also decreased when ${\alpha}-MSH$ was combined with LHE. In addition, the SOD-like activity was increased in a concentration-dependent manner in the treatment with the LHE, indicating signigicant activity at high concentrations, and the tyrosinase mRNA gene expression was decreased in both the LHE-treated group, the LHE and ${\alpha}-MSH-treated$ group. Conclusions : LHE seems to inhibit melanin synthesis through inhibition of tyrosinase activity and inhibition of tyrosinase mRNA gene expression. It also has the effect of promoting SOD-like activity and may be used clinically as a skin whitening agent in the future.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.9
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• pp.4038-4045
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• 2011

To investigate whitening Effects of Angelica dahurica Radix Ethanol Extract (ADEE), we used melan-a cell line, brown guinea pig, and HMB-45. We treated with ADEE of 6.25, 12.5, 25, and 50 ${\mu}g/m{\ell}$ concentration in order to evaluate the effect of ADEE on cell viability and on morphological observation of melan-a cells. Also we were induced the artificial tanning spots by 1,500 mJ/$cm^2$ of ultraviolet B radiation on the backs of brown guinea pigs (approximately 450~500g) and then the test agent of $30{\mu}{\ell}$ was applied on the spots twice a day, five days a week, for five weeks respectively. The visible whitening effect was evaluated once a week. At the end of the experiment, the animals were sacrificed under anesthetization. The artificial tanning spots were obtained by biopsy punch and stained with HMB-45 to observe the gp100 proteins which were melanosomes. Our results show that cell viability was not reduce at ADEE concentrations between 6.25 and 50 ${\mu}g/m{\ell}$, melanin synthesis and melanocyte dendricity were decreased in ADEE treated melan-a cells increasing ADEE concentration. In the gross observation, ADEE treated groups had lower pigmentation than the vehicle control groups. And in the histological observation, ADEE treated groups had lower melanocytes than the vehicle control groups. Also in the quantitative analysis of the gp100 proteins using image analysis software, ADEE treated groups had a significantly lower value (p<0.001) than the vehicle control group and this resultsagreed with the results of observation under microscope. From these results, weconcluded that ADEE had positive whitening effect.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.4 s.54
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• pp.295-304
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• 2005

Recently, arbutin, oil soluble licorice extact (GLY), ascorbyl glucoside (AA2G), and ethyl ascorbyl ether (EAE) have been widely used as functional whitening ingredients. To Investigate which combination between the above agents could be more effective for whitening effect, tyrosinase activity and MSH-induced melanin production in B-16 melanoma cells were investigated. Both GLY and arbutin dose-dependently inhibited purified tyrosinase activity. The inhibitory effects of GLY with AA2A or EAE on Drosinase activity were more potent than those of GLY alone, whereas that of arbutin with other ingreadients did not show those effects. In MSH-induced melanin production in B-16 melanoma cells, the mixture of Gly and EAE more significantly reduced melanin formation than Gly alone. Stability of mixture of GLY, arbutin, AA2A and EAE exposed at the temperature of $25^{\circ}C\;or\;45^{\circ}C$ for 30 days were also investigated. All of the combinations of whitening agents did not show any critical changes in their composition stability. These data suggest that the combination of GLY and vitamin C derivatives such as AA2G and EAE may be useful for the promotion of whitening effect.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.43 no.4
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• pp.76-81
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• 2011

This study was carried out in order to develop a speciality functional coated paper, which can be recognized under a ultraviolet lamp. The special fluorescent whitening agent (FWA) which absorbs ultraviolet light (397~410 nm) and emits it as yellowish light (570~500nm) was used. The special FWA was applied in a coating color in two ways: (1) direct application to a coating color as a FWA; (2) application on pigment surface, drying it and application it as a fluorescence whitening pigments (colored pigments). The effects of the special FWA on coating color properties were tested and the distribution of colored pigments in coated paper was evaluated under a ultraviolet lamp. The results showed that the colored pigments didn't affect on coating color properties. Experimental results showed the possibility of producing a security coated paper using the special FWA. To produce a speciality functional paper, it was found to be proper to apply the special FWA in the form of colored pigment.

• Journal of Applied Biological Chemistry
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• v.58 no.3
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• pp.266-271
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• 2015

Recently many effort focused to understand the mechanical insights of melanogenesis to develop the agent for hyper-pigmentation. So this study was performed to investigate the depigmentation of Vitex rotundifolia. With B16F10 mouse melanoma cell, we have seen inhibition of the tyrosinase, MITF, TRP-1, TRP-2, and melanin synthesis, which eventually were dose dependently decreased by Vitex rotundifolia. Specially, Vitex rotundifolia decreased the protein levels of tyrosinase and TRP-1. In conclusion, Vitex rotundifolia showed the whitening activity in all the experiments mentioned above and we expect that it can be used for preventing melanin synthesis.

• Journal of Life Science
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• v.23 no.3
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• pp.383-388
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• 2013

This study was performed to determine the whitening effect of several solvent fractions of Diospyros kaki extracts. Fractions from ethanol extracts of D. kaki were prepared by a systematic fractionation procedure using hexane, ethyl acetate, butanol, and $H_2O$. The ethyl acetate fractions were used to evaluate the inhibitory effects on tyrosinase activity and melanin synthesis in B16F10 melanoma cells. Ethyl acetate fractions suppressed the expression of tyrosinase, TRP-1 and TRP-2 in B16F10 melanoma cells. These results showed that ethyl acetate fractions of D. kaki could be developed as a skin whitening material in cosmetics.

• Natural Product Sciences
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• v.16 no.2
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• pp.133-139
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• 2010

In order to develop a new skin whitening agent, approximately 100 plant extracts were evaluated for their inhibitory activities against melanin biosynthesis in cultured mouse melanocyte melan-a cells. As a result, seven extracts exhibited over 50% inhibition of melanin synthesis compared to control at a concentration of $20\;{\mu}g/ml$. In particular, Aster ageratoides Turcz. var. ageratoides (branch, root, aerial, flower; $IC_{50}$ = 17.3, 6.1, 13.6, $12.9\;{\mu}g/ml$, respectively) and Physalis alkekengi var. francheti (leaf, unripen fructus, aerial; $IC_{50}$ = 6.5, 28.3, $23.9\;{\mu}g/ml$) markedly inhibited melanin synthesis. In addition, tyrosinase activity was monitored by the measurement of dopachrome formation from the oxidation of L-3,4-dihydroxyphenylalanine. Extracts of A. ageratoides Turcz. var. ageratoides (flower) and P. alkekengi var. francheti (leaf) showed the most potent tyrosinase inhibitory activity. These plants might be the potential candidate sources in the development of novel skin-whitening products.

• YAKHAK HOEJI
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• v.42 no.1
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• pp.39-45
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• 1998

Kojic acid, antimelanogenic agent, has been widely used in cosmetics to lighten the skin color. However, it has skin irritancy and instability against pH, temperature and light. To overcome these problems and optimize the molecular structure of kojic acid (KA), a prodrug, kojic acid monostearate(KMS), has been synthesized to modify the topical drug delivery in the point of sustained release of the parent drug via enzymatic hydrolysis during skin absorption. The prodrug was tested for enzymatic hydrolysis with cytosolic fraction of hairless mouse, skin. From the in vitro skin permeation study through hairless mouse skin, we found that KMS was retained in the skin and generated KA continuously by the skin esterase cleavage. In addition, topical formulations of o/w type creams and polyolprepolymer-containing cream were further tested for whitening effects using in vivo yellow skin guinea pig model.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.3
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• pp.271-279
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• 2021

Ultrasound-assisted extraction (UAE) conditions, including extraction time, extraction temperature, and ethanol concentration, were optimized to increase the total flavonoid content (TFC), tyrosinase inhibitory activity (TIA), and collagenase inhibitory activity (CIA) of Spirulina platensis through central composite design (CCD). For the optimization of the three dependent variables, a quadratic regression model was derived from 17 experimental sets for the simultaneous maximization of TFC, TIA, and CIA. The predicted optimal UAE conditions were 44.0 min of extraction time, 82.8℃ of extraction temperature, and 96.0% of ethanol concentration. Under these conditions, 0.93 mg QE/g DM of TFC, 81.9% of CIA, and 92.1% of TIA were predicted. The CCD-based UAE optimization enabled an increase in TFC, CIA, and TIA, thereby confirming that the S. platensis extract can be used in the development of a cosmetic material with skin whitening and anti-wrinkle effects.