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http://dx.doi.org/10.6114/jkood.2020.33.2.070

The Skin Whitening Effect of Gypsum Extract using B16F10 cell lines  

Kam, Eun-Young (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University)
Kang, Eun-Jeong (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University)
Ryu, Ji-Yeon (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University)
Park, Soo-Yeon (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University)
Jung, Min-Yeong (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University)
Kim, Jong-Han (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University)
Choi, Jung-Hwa (Dep. of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.33, no.2, 2020 , pp. 70-82 More about this Journal
Abstract
Objectives : Recently the aesthetic, especially skin whitening, is focused on. This study was designed to investigate the effects of Gypsum Extract(GE) on skin whitening using B16F10 cell lines. Methods : In this study, we investigated effect of GE on cell viability, melanin synthesis, Superoxide dismutase(SOD)-like activity and tyrosinase activity in vitro and in vivo. Results : In results, The proliferation of B16F10 cell lines was increased by GE concentration. But there was no statistical signification. Melanin synthesis was decreased except GE 125㎍/㎖ of treated group. And the melanin synthesis ratio was increased in group being treated α-MSH. But After treating GE, it was decreased compared to Non-GE treated group. In addition, the SOD-like activity was enhanced in more than GE 125㎍/㎖ of treated group. And the activity of tyrosinase in vitro was inhibited in GE 500㎍/㎖ and 1,000㎍/㎖ of treated groups. The activity of tyrosinase in vivo was reduced in both Arbutin 500㎍/㎖ of treated group and Gypsum 500㎍/㎖ of treated group compared to controled group. After being treated α-MSH, the activity of tyrosinase was enhanced. But after treating Arbutin 500㎍/㎖ or Gypsum 500㎍/㎖, it was inhibited being compared to the group of Non-medicament treated. Conclusion : The results of this study showed the effects of GE that can inhibit melanin synthesis and the activity of tyrosinase and enhance the SOD-like activity. It means that gypsum has the skin whitening effect.
Keywords
Gypsum extract(GE); B16F10; Skin whitening;
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Times Cited By KSCI : 8  (Citation Analysis)
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