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Inhibitory Effects of Plant Extracts on Tyrosinase Activity and Melanin Synthesis  

Park, Hyen-Joo (College of Pharmacy, Seoul National University)
Park, Kwang-Kyun (College of Dentistry, Yonsei University)
Hwang, Jae-Kwan (College of Engineering, Yonsei University)
Chung, Won-Yoon (College of Dentistry, Yonsei University)
Lee, Seung-Eun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science)
Lee, Sang-Kook (College of Pharmacy, Seoul National University)
Publication Information
Natural Product Sciences / v.16, no.2, 2010 , pp. 133-139 More about this Journal
Abstract
In order to develop a new skin whitening agent, approximately 100 plant extracts were evaluated for their inhibitory activities against melanin biosynthesis in cultured mouse melanocyte melan-a cells. As a result, seven extracts exhibited over 50% inhibition of melanin synthesis compared to control at a concentration of $20\;{\mu}g/ml$. In particular, Aster ageratoides Turcz. var. ageratoides (branch, root, aerial, flower; $IC_{50}$ = 17.3, 6.1, 13.6, $12.9\;{\mu}g/ml$, respectively) and Physalis alkekengi var. francheti (leaf, unripen fructus, aerial; $IC_{50}$ = 6.5, 28.3, $23.9\;{\mu}g/ml$) markedly inhibited melanin synthesis. In addition, tyrosinase activity was monitored by the measurement of dopachrome formation from the oxidation of L-3,4-dihydroxyphenylalanine. Extracts of A. ageratoides Turcz. var. ageratoides (flower) and P. alkekengi var. francheti (leaf) showed the most potent tyrosinase inhibitory activity. These plants might be the potential candidate sources in the development of novel skin-whitening products.
Keywords
Melanin; Tyrosinase; Skin Whitening; Physalis alkekengi var. francheti; Aster ageratoides Turcz. var. ageratoides;
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