• Bulletin of the Korean Chemical Society
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• 제26권7호
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• pp.1135-1137
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• 2005

• 한방안이비인후피부과학회지
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• 제25권1호
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• pp.1-11
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• 2012

Objective : Paeoniae radix alba(PRA) can enrich the blood and regulate menstruation, astringe yin and arrest sweating, calm the liver and arrest pain. This study was designed to investigate effects of PRA on skin whitening and elasticity using melanoma cells. Methods : In this experiment, effect of PRA on cell viability, inhibition of melanin synthesis and inhibitory effect on tyrosinase and elastase. Results : 1. More than $1,000\;{\mu}g/ml$ of PRA treated group showed lowered proliferation rates significantly compared to non-treated control group. 2. All of treated groups were lower levels of melanin synthesis respectively. 3. PRA did not show inhibitory effect on tyrosinase activities in vitro. But, PRA suppressed tyrosinase activities in B16F10 cells significantly. 4. PRA suppressed elastse type 1 activities in dose-dependent manner in vitro. But, PRA slightly suppressed elastase type 4 activities in vitro, and PRA also slightly suppressed elastase activities in vivo. Conclusion : These results suggest that PRA can inhibit melanin synthesis through ihhibitory action on tyrosinase activity and inhibt elastase activity, and also suggest that these results can be used for the study on maintaining skin whitening or elasticity.

• 한국응용과학기술학회지
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• 제26권2호
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• pp.110-116
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• 2009

In an attempt to find natural sources of antioxidants and whitening agents, Comparisons of the antioxidative and tyrosinase inhibitory activities of various ethanol extracts of Astragali Radix, Atractylodis Rhizoma Alba and Acanthopanacis Cortex were carried out. Comparison of the four ethanol extracts revealed that, Astragali Radix had the highest electron-donating ability(72.5%) and the highest SOD-like ability(26.1%). The xanthine oxidase experiment exhibited a hindrance effect of 88.5% in Atractylodis Rhizoma Alba, 81.1% in Acanthopanacis Cortex, 75.8% in Astragali Radix. A tyrosinase inhibitory activity assay was conducted to evaluate the whitening effects of the extracts, The tyrosinase inhibitory activity was 42.1% in the Acanthopanacis Cortex, 37.2% in the Atractylodis Rhizoma Alba, 6.0% in the Astragali Radix. Based on these results, we suggest that the ethanol extracts of Astragali Radix, Atractylodis Rhizoma Alba and Acanthopanacis Cortex can be used as food and cosmetic ingredients.

• 한방안이비인후피부과학회지
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• 제20권3호
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• pp.1-13
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• 2007

Objective : This study was performed to assess the whitening effect of Bombysis Corpus on melanin synthesis. Methods : The whitening effects of Bombysis Corpus were examined by in vitro melanin production assay. We assessed inhibitory effects of Bombysis Corpus on melanin-release from B16F10, on melanin production in B16F10, on mushroom tyrosinase activity in vitro, on tyrosinase activity in B16F10, effect of Bombysis Corpus on the expression tyrosinase, TRP-1, PKA, ERK-1 ERK-2, AKT-1, MITF in B16F10. Results : 1. Bombysis Corpus inhibited melanin-release, melanin production in B16F10. 2. Bombysis Corpus inhibited tyrosinase activity in vitro and in B16F10. 3. Bombysis Corpus suppressed the expression of tyrosinase, TRP-1 in B16F10. 4. Bombysis Corpus suppressed the expression of PKA in B16F10. 5. Bombysis Corpus suppressed the expression of ERK-1, ERK-2, AKT-1 in B16F10. 6. Bombysis Corpus suppressed the expression of MITF in B16F10. Conclusion : The study shows that Bombysis Corpus inhibited melanin production on the melanogenesis.

• 한국응용과학기술학회지
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• 제27권4호
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• pp.539-544
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• 2010

In this study, we evaluated the anti-oxidative, anti-wrinkle and whitening effect of Duchesnea indica (Andr.) Focke extract. Duchesnea indica (Andr.) Focke was extracted by two different solvents which were n-hexane and ethyl acetate. The anti-oxidant activity was measured by free radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl radical). And the inhibitory activities of tyrosinase for whitening effect and collagenase for anti-wrinkle were investigated. For anti-oxidant activity and whitening activity, ethyl acetate fraction of Duchesnea indica (Andr.) Focke extract showed more significant activity than n-hexane fraction of Duchesnea indica (Andr.) Focke extract. For anti-wrinkle activity, ethyl acetate fraction of Duchesnea indica (Andr.) Focke extract exhibited strong inhibition effects compared with reference. Therefore, Duchesnea indica (Andr.) Focke extract may be useful as a new antioxidant and anti-aging agent.

• 한국응용과학기술학회지
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• 제31권1호
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• pp.136-142
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• 2014

In an attempt to find natural sources of antioxidants and whitening agents, comparisons of the antioxidative and tyrosinase inhibitory activities of various ethanol extracts of Cuscutae Semen, Rubi Fructus and Paeoniae Radix were carried out. Comparison of the three ethanol extracts revealed that, Paeoniae Radix had the highest electron-donating ability(79.3%),; however, Rubi Fructus had the highest SOD-like ability(31.1%). The xanthine oxidase experiment exhibited a hindrance effect of 74.3% in Cuscutae Semen, 80.4% in Rubi Fructus and 60.8% in Paeoniae Radix. A tyrosinase inhibitory activity assay was conducted to evaluate the whitening effects of the extracts, The tyrosinase inhibitory activity was 20.1% in the Cuscutae Semen, 54.2% in the Rubi Fructus, 56.3% in the Paeoniae Radix. Based on these results, we suggest that the ethanol extracts of Cuscutae Semen, Rubi Fructus and Paeoniae Radix can be used as food and cosmetic ingredients.

• 한방안이비인후피부과학회지
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• 제15권2호
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• pp.1-19
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• 2002

Fifteen herbs used in oral medicine were extracted in ethanol and screened for tooth whitening effect, radical scavenging activity, inhibition of inducible nitric oxide synthase activity and anti-bacterial activity on Streptoccocus mutans. The results are as followed: 1. The tooth whitening effect of toothpaste containing 1 $\%$ ethanol extracts of 15 herbs was tested in brushing method using artificially stained HAT tablet and bovine tooth specimens. Toothpaste containing 1$\%$ of Vucia unijuga, Illicium verum, Angelica dahurica and Piper longum extracts showed tooth whitening effects on both HAT tablet and bovine tooth specimens. But the others did not show a considerable activity. 2. The antioxidant activity of ethanol extracts of 15 herbs was tested using the method of 1,1-diphenyl-2-picryl hydrazyl (DPPH) reactivity. Two ethanol extracts of Cimicifuga heracleifolia and Phyllostachys nigra var. henonis were found to be the most effective on radical scavenging activity. C. heracleifolia and P. nigra var. henonis extracts removed 86$\%$ and 81 $\%$ of DPPH radical at 0.01$\%$, compared with butylated hydroxy toluene as positive control. 3. The inhibition activity of ethanol extracts on nitrate production In RAW264.7 cell stimulated by lipopolysacchride was tested using the Griess reagent. But all extracts did not inhibit nitrate production. 4. The antibacterial activity on Streptoccocus mutans was tested by paper disk method. But no one extract showed any anti - bacterial activity.

• 대한화장품학회지
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• 제41권2호
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• pp.151-157
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• 2015

본 연구에서는 기능성 화장품 소재 개발을 위하여 비파엽 추출물을 제조하고 효소 및 세포 활성평가를 진행하였다. 활성평가는 tyrosinase 측정법을 이용한 미백 효능, NO 측정법을 이용한 항염 효능에 대한 활성등을 분석하였다. 미백 효능 관련 tyrosinase 활성 저해는 10% 농도의 비파엽 초임계 추출물에서 미약한 활성을 나타내었다. 항염 효능 관련 NO 활성 저해 분석 결과, 초임계 추출물, 에탄올 환류 추출물, 열수 추출물의 순서로 NO 억제 활성을 나타내었다. MTT assay를 실시한 결과, 70% 에탄올 환류 추출물 5% 처리를 제외하고는 모든 농도에서 세포독성이 관찰되지 않았다. 미백 효능 관련 멜라닌 생성 저해는 5% 처리 농도에서 30%, 70% 에탄올 환류 추출물이 강하게 멜라닌 생성을 억제하는 결과를 보였다. 이상의 연구결과들로부터 비파엽 추출물 제조를 통한 우수한 항염 효능 및 미백 효능 관련 활성을 나타내는 추출조건을 도출하였으며, 향후 기능성 화장품 소재 개발을 위한 기초 연구용 자료로서의 역할을 제시할 수 있을 것이다.

• 약학회지
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• 제47권6호
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• pp.398-403
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• 2003

To investigate the relationship between structure and biological activity of phenylpropanoids, we measured effects of phenylpropanoids on anti-oxidant and whitening activity, In DPPH radical scavenging activity, caffeic acid analogues showed the significant anti-oxidant activity. Although phenylpropanoids did not inhibit purified-tyrosinase activity, they significantly inhibited tyrosinase activity and melanin production in MSH-stimulated B16 melanoma cells. However, phenylpropanoids did not affect tyrosinase expression in MSH-stimulated B16 melanoma cells, which suggest that inhibition of MSH-induced melanin production was due to tyrosinase inhibition mediated via other signal pathways but not expression of tyrosinase. Phenylpropanoids also significantly inhibited both hyaluronidase and elastase activity, suggesting that phenylpropanoids may be used as whitening, hydration and anti-wrinkling agents. Hydroxyl residue of aromatic ring in phenylpropanoids plays an important role in anti-oxidant and whitening activity.

• 한국식품영양과학회지
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• 제39권10호
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• pp.1418-1424
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• 2010

본 연구에서는 정공피 물 추출물의 미백 효과를 확인하기 위하여 항산화 효능을 검증하였고, 멜라닌 생성의 첫 단계인 tyrosinase 활성, mouse melanoma B16BL6 세포 생존율 및 TRP-1, TRP-2 mRNA 발현 정도를 측정하였다. 정공피 물추출물은 DPPH 및 hydroxy 라디칼 소거 작용을 통하여 높은 항산화 효능을 나타내었고, tyrosinase, TRP-1, TRP-2의 활성을 효과적으로 억제하는 것을 확인하였다. 이는 정공피 물 추출물이 직접적으로 B16BL6 melanoma 세포의 tyrosinase 단백질의 활성을 저해시킴으로써 멜라닌 생성을 억제하는데 효과적임을 의미한다. 결론적으로 정공피 추출물은 항산화 활성과 미백 효과가 우수하여 기능성 화장품의 천연 소재로서 활용 가능성이 매우 높은 것으로 판단된다.