• Title/Summary/Keyword: whitening agent

Search Result 239, Processing Time 0.029 seconds

Whitening Activities of the Halophyte L. tetragonum (Thunberg) A. A. Bullock Extract in B16F10 Melanoma Cells (염생식물 갯질경 추출물의 미백 활성)

  • Kim, Min-Jin;Hyun, Kwang Hee;Kim, Ji-Hye;Han, Dong Hwan;Kim, Seung-Young;Lee, Nam Ho;Hyun, Chang-Gu
    • KSBB Journal
    • /
    • v.32 no.3
    • /
    • pp.218-233
    • /
    • 2017
  • To identify the natural and novel cosmeceutical ingredients which have whitening function, we examined the effects of tyrosinase activity and melanin production on halophyte Limonium tetragonum (Thunb.) A. A. Bullock extracts. As a result, the extracts showed whitening effect with no cytotoxicity. Melanin contents of B16F10 melanoma cells were decreased in a dose-dependent manner at 50, 100, $200{\mu}g/mL$ treatment of the extracts. In tyrosinase activity inhibition test, L. tetragonum (Thunb.) A. A. Bullock extracts showed decreased tyrosinase activity as the concentration of ${\alpha}-MSH$ was increased. Furthermore, it was observed that the tyrosinase and MITF expression was significantly downregulated by adding L. tetragonum (Thunb.) A. A. Bullock extracts in ${\alpha}-MSH$, a melanogenesis inducing material, treated B16F10 melanoma cells. These results indicate that L. tetragonum (Thunb.) A. A. Bullock extract might be an effective whitening agent by inhibit melanin formation.

Antioxidative Activity and Antiaging Effects of Duchesnea indica (Andr.) Focke extract (뱀딸기추출물의 항산화 활성 및 항노화에 관한 연구)

  • Roh, Eon-Joo;Park, Si-Hyang;Hwang, Su-Mi;Ro, Hyeon-Su;Kim, Byung-Kwan
    • Journal of the Korean Applied Science and Technology
    • /
    • v.27 no.4
    • /
    • pp.539-544
    • /
    • 2010
  • In this study, we evaluated the anti-oxidative, anti-wrinkle and whitening effect of Duchesnea indica (Andr.) Focke extract. Duchesnea indica (Andr.) Focke was extracted by two different solvents which were n-hexane and ethyl acetate. The anti-oxidant activity was measured by free radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl radical). And the inhibitory activities of tyrosinase for whitening effect and collagenase for anti-wrinkle were investigated. For anti-oxidant activity and whitening activity, ethyl acetate fraction of Duchesnea indica (Andr.) Focke extract showed more significant activity than n-hexane fraction of Duchesnea indica (Andr.) Focke extract. For anti-wrinkle activity, ethyl acetate fraction of Duchesnea indica (Andr.) Focke extract exhibited strong inhibition effects compared with reference. Therefore, Duchesnea indica (Andr.) Focke extract may be useful as a new antioxidant and anti-aging agent.

Anti-oxidant Activity and Whitening Activity of Bamboo Extracts (대나무 추출액의 항산화 작용과 미백작용)

  • Song, Ho-Sun;Moon, Hyo-Jin;Park, Byoung-Eun;Choi, Bang-Sil;Lee, Dong-Ja;Lee, Ji-Yun;Kim, Chang-Jong;Sim, Sang-Soo
    • YAKHAK HOEJI
    • /
    • v.51 no.6
    • /
    • pp.500-507
    • /
    • 2007
  • To investigate the possibility of development as a whitening agent using bamboo extracts (Phyllostachys nigra var. henonis), we measured DPPH assay, NBT/XO assay, intracellular ROS scavenging assay, tyrosinase assay and MSH-induced melanin production in B-16 cells. Bamboo extracts had dose-dependently anti-oxidant activity in DPPH, NBT/XO and intracellular ROS assay. Bamboo extracts appear to inhibit xanthine oxidase directly. Bamboo extracts inhibited not only purified tyrosinase activity but also inhibited tyrosinase activity and melanin production in B16 melanoma cells stimulated by $1{\mu}M\;{\alpha}$-MSH. Anti-oxidant activity and cytotoxicity of ethyl acetate fraction was more potent than those of water fraction, whereas whitening effect of water fraction was stronger than ethyl acetate fraction. Therefore, these results suggest that water fraction of bamboo extracts may be useful for the development as whitening agents reducing cytotoxicity.

Antaroide, a Novel Natural Nine-Membered Macrolide, Inhibits Melanin Biosynthesis in B16F10 Murine Melanoma Cells

  • Ryu, Min-Ji;Baek, Eun-Ki;Kim, Soyeon;Seong, Chi Nam;Yang, Inho;Lim, Kyung-Min;Nam, Sang-Jip
    • Biomolecules & Therapeutics
    • /
    • v.29 no.1
    • /
    • pp.98-103
    • /
    • 2021
  • The demand for natural substances with anti-melanogenic activity is increasing due to the recent interest in skin whitening. Intensive investigation on the culture broth of Streptomyces sp. SCO-736, a marine bacterium from the Antarctica coast, has led to the isolation of a new natural product named antaroide (1). The chemical structure was established through the interpretation of MS, UV, and NMR spectroscopic data. Antaroide is a nine-membered macrolide with lactone and lactam moieties. To investigate its applicability in skin whitening cosmetics, its anti-melanogenic activity in B16F10 murine melanoma cells was examined. As a result, antaroide displayed strong inhibitory activities against melanin synthesis and also attenuated the dendrite formation induced by the α-melanocyte stimulating hormone (α-MSH). Antaroide suppressed the mRNA expression of the melanogenic enzymes such as tyrosinase, TRP-1 and TRP-2. This suggests that it may serve as a transcriptional regulator of melanogenesis. Collectively, the discovery of this novel natural nine-membered macrolide and its anti-melanogenic activity could give new insights for the development of skin whitening agents.

CLINICAL STUDY OF SHADE IMPROVEMENT AND SAFETY OF POLYMER-BASED PEN TYPE BlancTis Forte WHITENING AGENT CONTAINING 8.3% CARBAMIDE PEROXIDE (8.3% Carbamide Peroxide 함유 펜 형 자가미백제인 BlancTis Forte의 색조개선 및 안전성에 관한 임상연구)

  • Lee, Jin-Kyung;Min, Sun-Hong;Hong, Sung-Tae;Oh, So-Ram;Chung, Shin-Hye;Hwang, Young-Hye;You, Sung-Yeop;Bae, Kwang-Shik;Baek, Seung-Ho;Lee, Woo-Cheol;Son, Won-Jun;Kum, Kee-Yeon
    • Restorative Dentistry and Endodontics
    • /
    • v.34 no.2
    • /
    • pp.154-161
    • /
    • 2009
  • This clinical study evaluated the whitening effect and safety of polymer based-pen type BlancTis Forte (NIBEC) containing 8.3% carbamide peroxide. Twenty volunteers used the BlancTis Forte whitening agent for 2 hours twice a day for 4 weeks. As a control. Whitening Effect Pen (LG) containing 3% hydrogen peroxide was used by 20 volunteers using the same protocol. The change in shade (${\Delta}E^*$, color difference) was measured using $Shadepilot^{TM}$ (DeguDent) before, during, and after bleaching (2 weeks, 4 weeks, and post-bleaching 4 weeks). A clinical examination for any side effects (tooth hypersensitivity or soft tissue complications) was also performed at each check-up. The following results were obtained. 1. Both the experimental and control groups displayed a noticeable change in shade (${\Delta}E$) of over 2. No significant differences were found between the two groups (p > 0.05), implying that the two agents have a similar whitening effect.2. The whitening effect was mainly due to changes in a and b values rather than in L value (brightness). The experimental group showed a significantly higher change in b value, thus yellow shade, than the control (p < 0.05). 3. None of the participants complained of tooth hypersensitivity or soft tissue complications, confirming the safety of both whitening agents.

Whitening Effect and Mechanism of Aerial Part of Pueraria lobata (갈만 추출물의 미백활성 및 기전 연구)

  • Kim, Da Eun;Hwang, Yeon Sil;Chang, Bo Yoon;Han, Ji Hye;Kim, Dae Sung;Kim, Hye Soo;Cho, Hyoung Kwon;Kim, Sung Yeon
    • Korean Journal of Pharmacognosy
    • /
    • v.47 no.4
    • /
    • pp.343-351
    • /
    • 2016
  • The purpose of this study was to evaluate the whitening effect of aerial part of Pueraria lobata and mechanisms. Aerial part of Pueraria lobata, dose-dependently reduced the melanin content. Aerial part of Pueraria lobata, significantly decreased cellular tyrosinase activity, while there was not any effect on tyrosinase in cell-free conditions. To elucidate the mechanisms behind the aerial part of Pueraria lobata, treated melanogenesis regulation, the expressions of melanogensis related genes, proteins, and the activity of ${\alpha}-glucosidase$ were determined. Aerial part of Pueraria lobata, significantly inhibited gene and protein levels of MITF, tyrosinase and TRP-1. It suppressed the ${\alpha}-glucosidase$, leading to inhibition on the maturation of tyrosinase. Also aerial part of Pueraria lobata, was observed to have the high antioxidant activity. These results suggested that whitening effect of aerial part of Pueraria lobata, should be due to the down-regulation of MITF, tyrosinase and TRP-1 expression and the intercepting maturation of tyrosinase through suppressing ${\alpha}-glucosidase$. Another should be the high anti-oxidant activity. The findings show the possibility that aerial part of Pueraria lobata, can be used as a potential skin-whitening agent.

Trend of Depigmenting Research Based on Patent Analysis (특허분석으로 본 미백 연구의 기술 동향)

  • Kim, Eun-Ki;Lee, Hyang-Bok;Lee, Haeng-Byoung;Lee, Cheo-Young
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.33 no.4
    • /
    • pp.209-217
    • /
    • 2007
  • Melanin plays an important role in protecting human skin from UV radiation and determines the race and skin color. Melanin is also major target for developing skin-whitening cosmeceuticals. Recently, as the market size of skin-whitening cosmeceuticals has rapidly expanded, related researches and developments are also focused on maximizing the safety and efficacy. Also, patents of skin-whitening materials have been increasing steadily for ensuring the competitive power. Patent also shows the research trend of industry and institutes. In this review, we analyze the trend of research and development based on the patent application of skin-whitening cosmeceuticals.

Whitening Effect of the Extracts from Juglans mandshurica (가래나무 추출물의 미백효과)

  • Lee, Gye-Won;Lee, Ju-Yeon;Cho, Young-Ho
    • KSBB Journal
    • /
    • v.25 no.1
    • /
    • pp.18-24
    • /
    • 2010
  • Juglans mandshurica belongs to the family Juglandaceae is known to contain a wide range of pharmacological activities including anti-cancer, anti-inflammation, astringent, and anti-human immunodeficiency virus-type 1 (HIV-1). Melanogenesis refers to the biosynthesis of melanin pigment in melanocytes. In this study, to investigate the whitening activity of the extracts from Juglans mandshurica, we measured effects on a tyrosinase activity, a melanogenesis, and a tyrosinase synthesis in the B16/BL6 melanoma cells and an antioxidant activity. The extracts significantly scavenged a 1,1-diphenyl-2-picrylhydrazyl (DPPH) and a superoxide anion radicals in a dose-dependent manner with a $SC_{50}$ value of $20\;{\mu}g/mL$ and $25\;{\mu}g/mL$, respectively. Also, the tyrosinase activity and melanogenesis were significantly inhibited by the extracts. Furthermore, the synthesis of tyrosinase protein was significantly decreased by the extracts in enzyme-linked immunosorbent assay. Double blind study on the clinical efficacy of a cream containing 2% of the extracts showed that the extracts have a significant skin whitening effect. Therefore, this study demonstrates that the extracts from Juglans mandshurica may be useful as a potential agent for skin whitening.

UV Protection and Whitening Effects of Collagen Isolated from Outer Layer of the Squid Todarodes pacificus (동해 오징어 (Todarodes pacificus) 껍질 유래 콜라겐의 UV 보호능 및 미백효과)

  • Kwon, Min-Chul;Qadir, Syed Abdul;Kim, Hyo-Sung;Ahn, Ju-Hee;Cho, Nam-Hyun;Lee, Hyeon-Yong
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.41 no.1
    • /
    • pp.7-12
    • /
    • 2008
  • To develop a whitening agent, cytotoxicity of the soluble collagen isolated from Todarodes pacificus (CIT) was evaluated. CIT tested for cytotoxicity on human dermal fibroblast (CCD-986sk) was exhibited very low cytotoxicity. Because tyrosinase is the key enzyme for melanin biosynthesis, the use of various tyrosinase inhibitors is a common practice for whitening purpose in cosmetics. Tyrosinase inhibitory activity and melanin production assay were measured to confirm the whitening effect. The inhibitory effect of MMP-1 in UV-irradiated human dermal fibroblast was also performed. CIT showed strong inhibition potency on tyrosinase by 51.5% at 0.2 mg/mL which increased the inhibition by increasing the concentration of CIT, and showed 69.1% inhibition at 1.0 mg/mL. CIT showed strong inhibition effect on melanin production with 82% at 1.0 mg/mL. The CIT also reduced about 76% expression of MMP-1 in UV-irradiated CCD-986sk cell at 1.0 mg/mL. From the preliminary observations, we suggest that the collagen isolated from CIT could be a potential source of natural skin-whitening and anti-aging agents for the photo-damaged skin.

The Antioxidant and Skin Whitening Effect of Withania somnifera (Winter Cherry) (윈터체리 추출물의 항산화 및 미백 개선 효과)

  • Kim, Dae Yong;Kim, Mee Kyung;Kim, Bong-Woo
    • Journal of Food Hygiene and Safety
    • /
    • v.30 no.3
    • /
    • pp.258-264
    • /
    • 2015
  • Withania somnifera has been used in folk medicine to treat various ailments for centuries. In this studies to investigate the whitening effect of Withania somnifera extracts as an active ingredient for whitening cosmetics, the antioxidant capacity and the effects of Withania somnifera extracts on melanogenesis in B16-F10 melanoma cells were identified. Withania somnifera extracts significantly reduced both tyrosinase activity and melanin content in a concentration-dependent manner. Furthermore, it was found that Withania somnifera extracts decreased ${\alpha}-MSH$ (melanocyte-stimulating hormone)-induced tyrosinase activity and MITF(microphthalmia associated transcription factor) protein expression. These data indicate that Withania somnifera extracts attenuate ${\alpha}$-MSH-stimulated melanin synthesis by modulating MITF expression and that they may be a useful therapeutic agent for treating hyperpigmentation and an ingredient of whitening cosmetics.