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http://dx.doi.org/10.5657/kfas.2008.41.1.007

UV Protection and Whitening Effects of Collagen Isolated from Outer Layer of the Squid Todarodes pacificus  

Kwon, Min-Chul (College of Bioscience and Biotechnology, Kangwon National University, Research Institute of Bioscience and Biotechnology, Kangwon National University)
Qadir, Syed Abdul (College of Bioscience and Biotechnology, Kangwon National University, Research Institute of Bioscience and Biotechnology, Kangwon National University)
Kim, Hyo-Sung (College of Bioscience and Biotechnology, Kangwon National University, Research Institute of Bioscience and Biotechnology, Kangwon National University)
Ahn, Ju-Hee (College of Bioscience and Biotechnology, Kangwon National University, Research Institute of Bioscience and Biotechnology, Kangwon National University)
Cho, Nam-Hyun (Hudem Food Company)
Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National University, Research Institute of Bioscience and Biotechnology, Kangwon National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.41, no.1, 2008 , pp. 7-12 More about this Journal
Abstract
To develop a whitening agent, cytotoxicity of the soluble collagen isolated from Todarodes pacificus (CIT) was evaluated. CIT tested for cytotoxicity on human dermal fibroblast (CCD-986sk) was exhibited very low cytotoxicity. Because tyrosinase is the key enzyme for melanin biosynthesis, the use of various tyrosinase inhibitors is a common practice for whitening purpose in cosmetics. Tyrosinase inhibitory activity and melanin production assay were measured to confirm the whitening effect. The inhibitory effect of MMP-1 in UV-irradiated human dermal fibroblast was also performed. CIT showed strong inhibition potency on tyrosinase by 51.5% at 0.2 mg/mL which increased the inhibition by increasing the concentration of CIT, and showed 69.1% inhibition at 1.0 mg/mL. CIT showed strong inhibition effect on melanin production with 82% at 1.0 mg/mL. The CIT also reduced about 76% expression of MMP-1 in UV-irradiated CCD-986sk cell at 1.0 mg/mL. From the preliminary observations, we suggest that the collagen isolated from CIT could be a potential source of natural skin-whitening and anti-aging agents for the photo-damaged skin.
Keywords
Todarodes pacificus; Collagen; MMP-1; Tyrosinase;
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