• Title/Summary/Keyword: white-rot fungi

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Cloning of a Laccase Gene Fragment from Ganoderma lucidum (영지버섯 Laccase 유전자의 구리결합부위 I과 IV사이 지역의 클로닝)

  • 조지현;최형태;김경훈
    • Korean Journal of Microbiology
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    • v.36 no.3
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    • pp.192-195
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    • 2000
  • Degenerate primevs corresponding to the consensus sequences of the copper-binding regions in the N- and Cterminal domains of fungal laccases were used to isolate laccase gene-specific sequences froin a white rot rungus Ganodern~a lucidrm w-hich has been known to strengthen the imnnne system. A 1.6 Kbp fragment was amplified by PCR and its base sequence was detenuiued. Locating seven iutrous within the base sequence, we could deduce its amino acid sequence. The nucleotide sequence witl~out introlls was 47Y0 identical to that of lee1 gene of Pametes wllosa; lhe identity in amino acid sequences of the two was 7994 The deduced amino acid seqoence was also sunilar to those of Coriolus versicolo~ kc3 (79%); Co~,iolz~s hirsutus phenolouiduse (78%), Trainetes vel.srcoloi. lccl (77%), Trametes ~!i/Iosa Ice2 (77%) and Trametes vemicolor kc4 (66%).

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Ralstonia pickettii Enhance the DDT Biodegradation by Pleurotus eryngii

  • Purnomo, Adi Setyo;Maulianawati, Diana;Kamei, Ichiro
    • Journal of Microbiology and Biotechnology
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    • v.29 no.9
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    • pp.1424-1433
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    • 2019
  • DDT is a hydrophobic organic pollutant, which can be bio-accumulated in nature and have adverse consequences on the physical condition of humans and animals. This study investigated the relationship between the white-rot fungus Pleurotus eryngii and biosurfactant-producing bacterium Ralstonia pickettii associated with the degradation of DDT. The effects of R. pickettii on fungal development were examined using in vitro confrontation assay on a potato dextrose agar (PDA) medium. R. pickettii culture was added to the P. eryngii culture at 1, 3, 5, 7, and 10 ml ($1ml{\approx}1.44{\times}10^{13}CFU$). After 7 d incubation, about 43% of the initial DDT ($12.5{\mu}M$) was degraded by the P. eryngii culture only. The augmentation of 7 ml of R. pickettii culture revealed a more highly optimized synergism with DDT degradation being approximately 78% and the ratio of optimization 1.06. According to the confrontational assay, R. pickettii promoted the growth of P. eryngii towards the bacterial colony, with no direct contact between the bacterial cells and mycelium (0.71 cm/day). DDD (1,1-dichloro-2,2-bis(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene), and DDMU (1-chloro-2,2-bis(4-chlorophenyl) ethylene) were identified as metabolic products, indicating that the R. pickettii could enhance the DDT biodegradation by P. eryngii.

First report of white rot on a wild gu1ic(Allium monanthum) caused by Sclerotium cepivorum and Sclerotium sp.

  • Cho, Weon-Dae;Hong, Sung-Ki;Kim, Yong-Ki;Kim, Woo-Sik;Jee, Hyeong-Jin
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.131.2-132
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    • 2003
  • White rot on garlic caused by Sclerotium cepivorum firstly occurred at Goheoung, Jeonnam in 1998. Thereafter, the disease rapidly spread throughout the country except Gangwon and became a major limiting factor for the cultivation of various Allium species such as garlic, onion, and welsh onion. The disease that has not been reported on a wild garlic(Allium monanthum) previously occurred severely at Seosan, Choongnam in 2003. Among cultivation areas in the region, 10.7% were infected by the disease and the ratio of diseased plant reached up to 55.0% in some heavily infected fields. Two species of Sclerotium were consistently isolated from infected samples and identified as S. cepivorum or another Sclerotium sp. Averaged size of sclerotium of the former was 455.0x562.2 urn, while the later was 374.4${\times}$347.2$\mu\textrm{m}$. Patogenicity to Allium species and mycological characteristics such as sclerotium size, growth temperature, and microconidia of the fungi were similar to those reported on other Allium species previously. Consequently, the wild garlic is a newly reported host of the two pathogenic fungi in Korea.

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Ganoderma lucidum균 전처리를 이용한 볏짚의 상압.소다펄프화

  • 주용찬;강진하
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.34 no.4
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    • pp.51-60
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    • 2002
  • This study was carried out to develop the biochemical pulping method to enhance energy saving and decrease the capital cost through the soda pulping under atmospheric pressure (100%). Nonwood substrates, rice straw, were pretreated by white-rot fungi, Ganoderma Iucidum. It has acquired several basic data that can be applied in bio-soda pulping. The results of this study were as follow. Without any nutrients or with glucose, N and glucose+N the weight losses of rice straws inoculated by Canoderma Iucidum were 8.5~29.8%, 9.3~32% 11.8~30.1% and 11.8~24.4% respectively for 5, 10, 15, 20, 25 and 30 days incubation. The more extending incubation periods, the more increasing weight losses. The yield of untreated rice saw was 54.8% after pulping. When any nutrients was not added or glucose, N and glucose+N were added for the pretreatment, the total yields were ranged 50.6~38.5%, 48.6~34.4%, 47.2~38.4% and 49.5~42.6% respectively for 5, 10, 15, 20, 25 and 30 days incubation. The yields were gradually decreased based on extending of incubation. The physical properties of rice straw soda pulps without fungal treatment, the density, breaking length, burst index, tear index and folding endurance were 0.24 g/$\textrm{cm}^3$, 2.32 km, 0.91 kPa $m^2$/g, 56.7 mN.$m^2$/g and 35 times, respectively. After pretreatment without any nutrients or with glucose, N and glucose+N as nutrients the density was 0.27~0.30 g/$\textrm{cm}^3$, the breaking length 3.14~5.25 km, burst index 1.42~2.78 kPa.$\textrm{m}^2$/g, tear index45.8~64.5 mN.$\textrm{m}^2$/g and folding endurance 47~288 times at all incubating periods when pulping was done. The physical properties were increased with the increasing incubation periods. However, when glucose+N was added, the physical properties were shown superior results each incubating duration.

Decoorizatiion of Kraft Pulp Bleaching Effluent by White -rot Fungi (백색부후균에 의한 크라프트 펄프 표백폐수의 탈색)

  • 조남석;이재원;박종문;최태호;안드레레오노비치
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.31 no.4
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    • pp.58-68
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    • 1999
  • This experiment was to investigate decoloization characteristics of E1 effluents from the bleaching plant of pulp mill with three white-rot fungi(Trametes versicolor, Ganoderma appanatum and Pleurotus ostreatus).In addition, the effect of carbon and nitrogen resources was discussed on its decolorization. The color removal of E1 effluent during shaking and stationary cultures were 72% and 80%, respectively. Stationary culture was more effective on decolorization of E1 effluent compared to the shaking culture. The optimum inoculum weight was 1.0g based on dry weight of mycelia . The decolorization medium I showed 88% of the color removal of E1 effluent with in one day cultivation of T.versicolor and P.ostreatus . Color removal was increased from 87% to 90%. T.versicolor and P.ostreatus by the addition of 0.5% glucose. By addition of nitorgen sources(ammonium sulfate and ammonium choride), medium was much higher than that of carbon source. With 0.1% ammoniumm sulfate, P.ostreatus and T.versicolor showed 94% and 92% of the color removal within one day of cultivation , respectively. On decolorization medium II, T.versicolor and P.ostretus were 94% of oclor removal with addition of carbon source. The addition of nitrogen source was much more efficient than that of carbon source. With 0.1% amminium chloride, T.versicolor and P.ostreatus showed 95% of its color removal . The decolorization medium II was higher color removal than medium I, and also MnP and laccase were produced. However, the decolorization medium I produced a little MnP and laccase activity. It could be suggested that MnP and laccase may play an important role in decolorization of E1 effluent.

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Genetic Transformation of Irpex lacterus and Phlebia tremellosa to an Antibiotic Resistance (아교버섯과 기계충버섯의 형질전환)

  • Kim, Yun-Jung;Kim, Myung-Kil;Song, Hong-Gyu;Choi, Hyoung-T.
    • Korean Journal of Microbiology
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    • v.43 no.2
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    • pp.147-149
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    • 2007
  • White-rot fungi which degrade lignin can also degrade diverse recalcitrant compounds such as polymeric dyes, explosives, pesticides, and endocrine disrupting chemicals. Lignin degrading enzymes are involved in the degradation reactions, and introduction of foreign genes into a white-rot fungus is required in order to increase the degrading capacity. Genetic transformation experiment has been carried out in Irpex lacteus and Phlebia tremellosa to an antibiotic resistance. The transformation yields were 50-70 transformants/${\mu}g$ DNA and 15-25 transformants/${\mu}g$ DNA in I. lacteus and P. tremellosa, respectively. The stable replication of the plasmid was confirmed by PCR using the plasmid-specific primers, and many mutants were generated during this integration in both fungi.

Protoplast Isolation and Genetic Transformation of Polyporus brumalis (겨울우산버섯의 원형질체 분리와 유전자 형질전환)

  • Ryu, Sun-Hwa;Kim, Myung-Kil
    • Korean Journal of Microbiology
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    • v.50 no.4
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    • pp.372-375
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    • 2014
  • This experiment was undertaken to investigate proper conditions for protoplast isolation and genetic transformation of the white rot fungi, Polyporus brumalis. The protoplasts were formed from mycelia at a frequency of $1{\times}10^7/ml$ with 0.5% Usukizyme. The transformation vector (pHYgpt) was constructed using hygromycin resistance gene (hph) for the selectable maker. The yield was 100-160 transformants/${\mu}g$ DNA in a transformation mediated by 40% polyethylene glycol solution with aurintricarboxylic acid, heparin and supermidine. The genomic integration of the pHYgpt was confirmed by hph-specific PCR and the expected amplified band appeared only in the transformants. These results could be an efficient tool in gene engineering of the genus polyporus.

Effect of $Zn^{2+}$ and Ferulic Acid on Laccase and Manganese Peroxidase Production by Funalia trogii (Funalia trogii에 의한 Laccase와 Manganese Peroxidase의 생산시 $Zn^{2+}$ 및 Ferulic Acid가 미치는 영향)

  • Park, Chul-Hwan;Han, Eun-Jung;Lee, Byung-Hwan;Lee, Jin-Won;Kim, Sang-Yong
    • KSBB Journal
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    • v.21 no.2
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    • pp.85-89
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    • 2006
  • Typical property of the white-rot fungi is their ability to degrade lignin and other aromatic compounds with non-specific extracellular enzyme. In this work, the modification of the strain(Funalia trogii ATCC 200800) and the culture condition was performed to enhance enzyme productivity. Single cell was separated by the protoplasts formation and several putative laccase and manganese peroxidase inducers were tested. By adopting the modified strain, enzyme productivity increased comparing with that of the original strain. Extracellular enzyme formation was highly stimulated by the addition of copper and various aromatic compounds in the glucose-based culture medium.

Enhanced Expression of Glucose 2-Oxidase in Phlebia tremellosa by Addition of Phthalates

  • Kim, Baik-Joong;Kim, Hye-Won;Choi, Hyoung-T.
    • Mycobiology
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    • v.39 no.1
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    • pp.64-66
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    • 2011
  • Most fungi possess several hydrogen peroxide-generating enzymes, glucose oxidase and pyranose oxidase. Pyranose oxidase can use glucose as its substrate to generate hydrogen peroxide. White rot fungi, which degrade diverse recalcitrant compounds, contain lignin-degrading enzymes, and lignin peroxidase and manganese peroxidase require hydrogen peroxide for their enzymatic reactions. In this study, we isolated a cDNA fragment of pyranose oxidase from Phlebia tremellosa using PCR and examined its expression under the degradation conditions of diethylphthalate (DEP). Pyranose oxidase expression was enhanced up to 30% by the addition of DEP, and this result supports the possible involvement of pyranose oxidase in the degradation of recalcitrant compounds.

Isolation of a Wood-rotting Fungus to Decolorize a Wide Range of Structurally Different Synthetic Dyes. (다양한 염료의 탈색이 가능한 목재부후균 분리)

    • Microbiology and Biotechnology Letters
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    • v.31 no.3
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    • pp.301-306
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    • 2003
  • Twenty-one different fungi were tested for their ability to decolorize a wide range of structurally different dyes. Twenty fungal strains were isolated from fruiting bodies which were collected at the Kwangneung National Arboretum, Korea. One fungal strain were isolated from a rotting wood at Soongsil University, Korea. Nine kinds of dyes were used: three anthraquinone dyes and six azo dyes. The five fungal strains, Laetiporus sulphureus, Polyporus arcularius. Auricularia polytricha, Stereum ostrea, and Bjerkandera sp. UK-l showed decolorization ability. Except Auricularia polytricha, the four fungal strains were wood rotting fungi, and belonged to Aphyllophorales. Bjerkandera sp. UK-I, which was a white rot fungus, could decolorize all kinds of dyes tested in this study, indicating this fungus is one of candidates for applying in biological methods of dye waste treatment.