Mycobiology

The Korean Society of Mycology (한국균학회)
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Enhanced Expression of Glucose 2-Oxidase in Phlebia tremellosa by Addition of Phthalates

  • Kim, Baik-Joong (Department of Biochemistry, Kangwon National University) ;
  • Kim, Hye-Won (Department of Biochemistry, Kangwon National University) ;
  • Choi, Hyoung-T. (Department of Biochemistry, Kangwon National University)
  • Received : 2010.10.25
  • Accepted : 2010.11.15
  • Published : 2011.03.31
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Abstract

Most fungi possess several hydrogen peroxide-generating enzymes, glucose oxidase and pyranose oxidase. Pyranose oxidase can use glucose as its substrate to generate hydrogen peroxide. White rot fungi, which degrade diverse recalcitrant compounds, contain lignin-degrading enzymes, and lignin peroxidase and manganese peroxidase require hydrogen peroxide for their enzymatic reactions. In this study, we isolated a cDNA fragment of pyranose oxidase from Phlebia tremellosa using PCR and examined its expression under the degradation conditions of diethylphthalate (DEP). Pyranose oxidase expression was enhanced up to 30% by the addition of DEP, and this result supports the possible involvement of pyranose oxidase in the degradation of recalcitrant compounds.

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References

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