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Genetic Transformation of Irpex lacterus and Phlebia tremellosa to an Antibiotic Resistance  

Kim, Yun-Jung (Division of Life Sciences, and Research Institute of Life Sciences, Kangwon National University)
Kim, Myung-Kil (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute)
Song, Hong-Gyu (Division of Life Sciences, and Research Institute of Life Sciences, Kangwon National University)
Choi, Hyoung-T. (Division of Life Sciences, and Research Institute of Life Sciences, Kangwon National University)
Publication Information
Korean Journal of Microbiology / v.43, no.2, 2007 , pp. 147-149 More about this Journal
Abstract
White-rot fungi which degrade lignin can also degrade diverse recalcitrant compounds such as polymeric dyes, explosives, pesticides, and endocrine disrupting chemicals. Lignin degrading enzymes are involved in the degradation reactions, and introduction of foreign genes into a white-rot fungus is required in order to increase the degrading capacity. Genetic transformation experiment has been carried out in Irpex lacteus and Phlebia tremellosa to an antibiotic resistance. The transformation yields were 50-70 transformants/${\mu}g$ DNA and 15-25 transformants/${\mu}g$ DNA in I. lacteus and P. tremellosa, respectively. The stable replication of the plasmid was confirmed by PCR using the plasmid-specific primers, and many mutants were generated during this integration in both fungi.
Keywords
genetic transformation; Irpex lacteus; Phlebia tremellosa; phosphinothricin resistance;
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