• Asian Pacific Journal of Cancer Prevention
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• 제15권3호
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• pp.1099-1104
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• 2014

Aims and Background: Ginsenoside Rh2, which exerts the potent anticancer action both in vitro and in vivo, is one of the most well characterized ginsenosides extracted from ginseng. Although its effects on cancer are significant, the underlying mechanisms remain unknown. In this study, we sought to elucidate possible links between ginsenoside Rh2 and phosphoglucose isomerase/autocrine motility factor (PGI/AMF). Methods: $KG1{\alpha}$, a leukemia cell line highly expressing PGI/AMF was assessed by western blot analysis and reverse transcription- PCR (RT-PCR) assay after transfection of a small interfering (si)-RNA to silence PGI/AMF. The effect of PGI/AMF on proliferation was measured by typan blue assay and antibody array. A cell counting kit (CCK)-8 and flow cytometry (FCM) were adopted to investigate the effects of Rh2 on PGI/AMF. The relationships between PGI/AMF and Rh2 associated with Akt, mTOR, Raptor, Rag were detected by western blot analysis. Results: KG1${\alpha}$ cells expressed PGI/AMF and its down-regulation significantly inhibited proliferation. The antibody array indicated that the probable mechanism was reduced expression of PARP, State1, SAPK/JNK and Erk1/2, while those of PRAS40 and p38 were up-regulated. Silencing of PGI/AMF enhanced the sensibility of $KG1{\alpha}$ to Rh2 by suppressing the expression of mTOR, Raptor and Akt. Conclusion: These results suggested that ginsenoside Rh2 suppressed the proliferation of $KG1{\alpha}$, the same as down-regulation of PGI/AMF. Down-regulation of PGI/AMF enhanced the pharmacological effects of ginsenoside Rh2 on KG1${\alpha}$ by reducing Akt/mTOR signaling.

• 한국콘텐츠학회논문지
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• 제16권5호
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• pp.463-476
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• 2016

1900년대 초는 서양 열강들이 아시아를 향해 본격적으로 진출한 시기로 당시 한국에는 외교관, 선교사, 의사 등 다양한 직업을 가진 외국인들이 들어왔다. 한국을 찾은 1900년대 초기의 외국인들에게 한국의 식생활은 호기심과 충격의 대상이었기에 그들은 한국인의 식생활에 대한 기록을 남겼다. 외국인들은 한국을 비교적 윤택하고, 오곡이 풍성한 매력적인 국가로 보았다. 한국인들에게 주식은 쌀이고, 중요한 양식으로 김치를 먹었으며, 다양한 곡식과 채소, 그리고 비빔밥을 먹었다. 한국에는 각종 육류와 생선, 미역, 약초(인삼)가 있었다. 외국인들은 한국인들이 식탐이 있다고 하면서, 밥을 고봉으로 많이 먹는다고 하였다. 한국인의 기본 식단으로는 밥 한그릇, 국 한 그릇, 김치와 간장, 다른 찬거리들이 있으며, 푸짐하게 상을 차려낼 때는 고깃국과 함께 날생선 말린 생선 튀긴 생선 중 한 가지가 올랐다. 한국에는 장독대가 있어서 간장, 된장, 고추장, 장아찌 등을 담가먹었다. 한국인에게는 특유의 옻냄새와 김치 양념냄새가 많이 났으며, 외국인들은 그 냄새를 림버거 치즈로 비유하면서 한국을 꼬랑내, 발냄새가 나는 나라로 언급하기도 하였다. 이처럼 1900년대 초 한국에 들어온 외국인들의 기록을 통해 한국의 식생활과 한국의 모습을 객관적으로 살펴볼 수 있었다.

• 한방안이비인후피부과학회지
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• 제13권2호
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• pp.76-111
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• 2000

I examined and referred to literatures of every generations on the nicknames, causes, herb medications and acupucture treatments of ptosis(上胞下垂) And then the results were obtained as follows. 1. The nicknames of ptosis(上胞下垂) are hyumok(휴목), chimpung(侵風), geompye(瞼廢), geompisubok(瞼皮垂覆), posu(胞垂) and bigwon(脾倦) which corresponds to blepharoptosis in Western Medicine. 2. The congenital ptosis(上胞下垂) is mostly caused by innate disposition(稟賦不足). The acquired ptosis(上胞下垂) is mostly caused by Qi sinking of Tri-energizer(中氣下陷). And besides this, there are Blood stasis due to Qi stagnancy(氣滯血瘀), invasion of the eyelid by wind(風邪入絡), Qi and Blood deficiency(氣血不足), Phlegm syndrome due to wind(風痰阻絡), Wind syndrome due to Yang hypertrophy(陽亢動風) and Stagnation of Liver Qi(肝氣鬱結). 3. In herb medication of ptosis(上胞下垂), Bojungikgitang(補中益氣湯) was used 14 times most and its effects are nourishing the spleen to promote the flow of Qi(健脾益氣) and elevating the YangQi and activation the meridian(升陽活絡). In the following, Insamyangyeongtang(人蔘養榮湯) was done 6 times and has effects of promoting the Qi and activating the blood(益氣養血) and of promoting blood circulation and restoring flow(活血通絡). The next, Jungyongtang(正容湯) appeared 5 times and this can expel wind, resolve phlegm and restore flow(祛風滌痰通絡). As single herb, Radix glycyrrhizae(甘草) was used 66 times most. Besides this, there are a few herbs used many times like Rhizoma atractylodis macrocephalae(白朮), Radix angelicae gogantis(當歸), Radix ginseng(人蔘). Radix astragali(황기) and Rhizoma cimicifugae(升麻). 4. In acupunture treatment, Chanzhu(撰竹) was used 19 times most. Besides this acupoint, there are some points choson frequently like Zusanli(足三里), Sanyinjiao(三飮交), Yangbai(陽白), Taiyang(太陽), Tongziliao(瞳子 ), Jingming(晴明), Hegu(合谷) and Fengchi(風池).

• 한국응용곤충학회지
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• 제23권4호
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• pp.209-214
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• 1984

1980년도부터 우리나라 Burley 종 잎담배산지에서 엽맥에 녹대병상 또는 괴저증상을 나타내는 새로운 병징이 관찰되기 시작하였다. 기주범위조사, 항혈청반응, 물리적성질조사, 복숭아혹진딧물에 의한 전염여부 및 바이러스 입자 관찰을 통해 이들은 서로 병징을 달리하는 PYY의 두가지 계통으로 밝혀졌다. 이 계통들은 잎담배포장에서 자연감염에 의해 나타난 주요병징에 따라 엽맥연대계통 (PVY-VB)과 엽맥괴저계통 (PVY-VN) 으로 명명하였다. PVY 항혈청과 이 계통들의 이병즙액을 반응시킨 결과 양성반응을 나타냈으며 그 반응에서는 두계통간의 차가 없었다. 이들과 병징이 유사한 tobacco etch virus 및 tobacco vein mottling virus 항혈청과의 반응에서는 음성반응을 나타냈다. 전자현미경에 의해 약 730 nm의 사상형 바이러스 입자가 관찰되었으며 그 형태와 크기에는 두 계통간 차가 없었다.

• Nutrition Research and Practice
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• 제10권3호
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• pp.259-264
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• 2016

BACKGROUND/OBJECTIVES: Stromal cell-derived growth factor 1 (SDF-1), also known as chemokine ligand 12, and chemokine receptor type 4 are involved in cancer cell migration. Compound K (CK), a metabolite of protopanaxadiol-type ginsenoside by gut microbiota, is reported to have therapeutic potential in cancer therapy. However, the inhibitory effect of CK on SDF-1 pathway-induced migration of glioma has not yet been established. MATERIALS/METHODS: Cytotoxicity of CK in C6 glioma cells was determined using an EZ-Cytox cell viability assay kit. Cell migration was tested using the wound healing and Boyden chamber assay. Phosphorylation levels of protein kinase C $(PKC){\alpha}$ and extracellular signal-regulated kinase (ERK) were measured by western blot assay, and matrix metallopeptidases (MMP) were measured by gelatin-zymography analysis. RESULTS: CK significantly reduced the phosphorylation of $PKC{\alpha}$ and ERK1/2, expression of MMP9 and MMP2, and inhibited the migration of C6 glioma cells under SDF-1-stimulated conditions. CONCLUSIONS: CK is a cell migration inhibitor that inhibits C6 glioma cell migration by regulating its downstream signaling molecules including $PKC{\alpha}$, ERK1/2, and MMPs.

• 대한한방소아과학회지
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• 제32권1호
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• pp.30-43
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• 2018

Objectives The purpose of this study is to analyze the clinical research literature of TCM (traditional Chinese medicine) which is applicable to pediatric fracture and to broaden the field about clinical application of Korean medicine treatment for pediatric fracture. Methods We searched randomized controlled trials about TCM treatment of pediatric fracture from the CNKI (China National Knowledge Infrastructure) January 2013 to December 2017. We searched the literature and analyzed the treatment methods and the results. Results 31 papers were selected from 63 studies. In most studies, the total effective rate of the treatment group was significantly higher than that of the control group, and the bone healing time of the treatment group was significantly lower than that of the control group. Methods of herb treatment include internal medicine (IM), fumigation (FG) and external application (EA). The most commonly used herb medicine were Angelicae Gigantis Radix (當歸), Carthami Flos (紅花), Drynaria Fortunei (骨碎補), Paeonia Lactiflora Pallas (芍藥), Persicae Semen (桃仁), Lycopodii Herba (伸筋草), Ligusticum Chuanxiong Hort (川芎), Olibanum (乳香), Salviae Miltiorrhizae Radix (丹蔘), and Panax Noto Ginseng (三七). Conclusions The use of herb medicine in pediatric fractures has resulted in faster and more effective restoration of fracture union than the Western medical therapy alone. Based on the results of this study, it is possible to widen the scope of Korean medicine if additional clinical studies on pediatric fracture were conducted.

• 농업과학연구
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• 제26권2호
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• pp.8-12
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• 1999

SEM을 사용하여 꽃노랑총채벌레, Frankliniella occidentalis,의 외부형태적 특징을 살펴보았다. 꽃노랑총채벌레의 촉각은 모두 8마디로 구성되어 있으며 길이는 0.25mm정도이다. 또한 3번째 마디와 4번째 마디는 부메랑 모양을 하고 있으며 5번째와 6번째 마디에는 길이가 $7{\mu}m$정도의 감각기가 있다. 8번째마디에는 8개의 감각기가 있다. 입틀은 비교적 앞으로 튀어나온 모양을 하고 있으며 많은 감각기가 분포하고 있는데 모두 14개의 감각기가 양쪽으로 배열되어 있다. 다리의 구조는 발톱을 가지고 있지 않다.

• Journal of Veterinary Science
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• 제22권2호
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• pp.18.1-18.12
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• 2021

Background: We previously elucidated the protective mechanism of Korean red ginseng oil (RGO) against Brucella abortus infection, and our phytochemical analysis revealed that palmitic acid (PA) was an abundant component of RGO. Consequently, we investigated the contribution of PA against B. abortus. Objectives: We aimed to investigate the efficacy of PA against B. abortus infection using a murine cell line and a murine model. Methods: Cell viability, bactericidal, internalization, and intracellular replication, western blot, nitric oxide (NO), and superoxide (O₂^-) analyses and flow cytometry were performed to determine the effects of PA on the progression of B. abortus infection in macrophages. Flow cytometry for cytokine analysis of serum samples and bacterial counts from the spleens were performed to determine the effect of PA in a mouse model. Results: PA did not affect the growth of B. abortus. PA treatment in macrophages did not change B. abortus uptake but it did attenuate the intracellular survivability of B. abortus. Incubation of cells with PA resulted in a modest increase in sirtuin 1 (SIRT1) expression. Compared to control cells, reduced nitrite accumulation, augmented O₂^-, and enhanced pro-inflammatory cytokine production were observed in PA-treated B. abortus-infected cells. Mice orally treated with PA displayed a decreased serum interleukin-10 level and enhanced bacterial resistance. Conclusions: Our results suggest that PA participates in the control of B. abortus within murine macrophages, and the in vivo study results confirm its efficacy against the infection. However, further investigations are encouraged to completely characterize the mechanisms involved in the inhibition of B. abortus infection by fatty acids.

• 대한본초학회지
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• 제28권6호
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• pp.155-160
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• 2013

Objectives : Gastric cancer is a leading cause of cancer-related deaths, worldwide. Houttuynia cordata Thunberg (H. cordata) has been used as a medicinal plants and it has an anti-cancer activity in human colorectal cancer and leukemic cancer. However, the potential anti-cancer activity and mechanisms of H. cordata for human gastric cancer cells have not been tested so far. Thus, this study examined the biological effects of H. cordata on the human gastric cancer cell line SNU-1 and AGS. Methods : Inhibition of cell proliferation and cell cycle by H. cordata was carried out by MTT assay and Muse cell cycle analysis and the expressions of protein associated with apoptosis and cell cycle regulation were investigated with Western blot analysis. Results : In MTT assay, the proliferation of SNU-1 and AGS cells was significantly inhibited by H. cordata in a time and dose dependent manner, Inhibition of cell proliferation by H. cordata was in part associated with apoptotic cell death, as shown by changes in the expression ratio of Bax to Bcl-2 by H. cordata. Also, H. cordata regulated the expression of cell cycle regulatory proteins such as pRb, cyclin D1, cyclin E, CDK4, CDK2, p21 and p15. Conclusion : The antiproliferative effect of H. cordata on SNU-1 and AGS gastric cancer cells revealed in this study suggests that H. cordata has intriguing potential as a chemopreventive or chemotherapeutic agent.

• 대한한방내과학회지
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• 제44권3호
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• pp.578-584
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• 2023

Objectives: This is a five-year survival and complete response (CR) report on rectal cancer treated with western medicine and Korean traditional medicine. Method: A 25-year-old woman diagnosed with rectal cancer visited ⃝⃝ Korean traditional medicine hospital after neoadjuvant concurrent chemoradiation therapy, chemotherapy, and low anterior resection with regional lymph node dissection. She was treated with Korean traditional medicine, including acupuncture, abdominal moxibustion, wild ginseng pharmacopuncture, and herbal medicine, which was based on integrated medicine therapy (IMT), from January 2018 to February 2022. The tumor size was measured by scanning with computed tomography (CT), magnetic resonance imaging, and positron-emission tomography/CT. Adverse events were evaluated using laboratory conclusion and National Cancer Institute Common Terminology Criteria for Adverse Events version 5.0. Result: During four years of treatment, IMT maintained safety. The patient finally reached five-year survival without any recurrence or complication (CR) on March 11, 2022. Conclusion: We suggest that an integrative approach including Korean traditional medicine can be a meaningful treatment option for rectal cancer. Further studies should be performed to establish the proper treatment protocol of integrative medicine for rectal cancer.