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http://dx.doi.org/10.4162/nrp.2016.10.3.259

Compound K attenuates stromal cell-derived growth factor 1 (SDF-1)-induced migration of C6 glioma cells  

Kim, Hyuck (Department of Diagnostics, College of Korean Medicine, Dongguk University)
Roh, Hyo Sun (Department of Acupoint, College of Korean Medicine, Dongguk University)
Kim, Jai Eun (Department of Pathology, College of Korean Medicine, Dongguk University)
Park, Sun Dong (Department of Prescription, College of Korean Medicine, Dongguk University)
Park, Won Hwan (Department of Diagnostics, College of Korean Medicine, Dongguk University)
Moon, Jin-Young (Department of Acupoint, College of Korean Medicine, Dongguk University)
Publication Information
Nutrition Research and Practice / v.10, no.3, 2016 , pp. 259-264 More about this Journal
Abstract
BACKGROUND/OBJECTIVES: Stromal cell-derived growth factor 1 (SDF-1), also known as chemokine ligand 12, and chemokine receptor type 4 are involved in cancer cell migration. Compound K (CK), a metabolite of protopanaxadiol-type ginsenoside by gut microbiota, is reported to have therapeutic potential in cancer therapy. However, the inhibitory effect of CK on SDF-1 pathway-induced migration of glioma has not yet been established. MATERIALS/METHODS: Cytotoxicity of CK in C6 glioma cells was determined using an EZ-Cytox cell viability assay kit. Cell migration was tested using the wound healing and Boyden chamber assay. Phosphorylation levels of protein kinase C $(PKC){\alpha}$ and extracellular signal-regulated kinase (ERK) were measured by western blot assay, and matrix metallopeptidases (MMP) were measured by gelatin-zymography analysis. RESULTS: CK significantly reduced the phosphorylation of $PKC{\alpha}$ and ERK1/2, expression of MMP9 and MMP2, and inhibited the migration of C6 glioma cells under SDF-1-stimulated conditions. CONCLUSIONS: CK is a cell migration inhibitor that inhibits C6 glioma cell migration by regulating its downstream signaling molecules including $PKC{\alpha}$, ERK1/2, and MMPs.
Keywords
Panax ginseng; compound K; SDF-1; anti-tumor; C6 glioma;
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