• 한국균학회소식:학술대회논문집
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• 2018.05a
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• pp.11-11
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• 2018

Cryphonectria parasitica, chestnut blight fungus, has a characteristic of decreasing pathogenicity when infected with Cryphonectria hypovirus 1. C. parasitica is known to be one of the most representative model systems used to observe the interaction between viruses, plants and fungi. The mitogen-activated protein kinase (MAPK) pathway, which is well conserved in various organisms ranging from yeast to humans, functions in relaying phosphorylation-dependent signals within MAPK cascades to diverse cellular functions involved in the regulation of pheromone, cell wall integrity, and osmotolerance in filamentous fungi. Several genes in the MAPK pathway were revealed to be regulated by hypovirus, or to be involved in pathogenicity in C. parasitica. Among these pathways, the CWI pathway has aroused interest because CpBck1, an ortholog of yeast Bck1 (a CWI MAPKKK), was previously reported to be involved in cell wall integrity and sectorization. Interestingly, sporadic sectorization was observed in the CpBck1 mutant and sectored phenotypes were stably inherited in the progeny that were successively transferred from sectored mycelia. In this study, we analyzed the biological function of CpSlt2, downstream gene of CpBck1, to confirm whether the sectorization phenomenon occurred in the specific single gene or cell wall integrity (CWI) pathway. As results, the CpSlt2-null mutant exhibited marked changes in colonial growth, near absence of conidiation and aerial hyphae, abnormal pigmentation, CWI-related phenotypic defects, and dramatically impaired virulence. As cultivation of the mutant strains progressed, the majority of the colonies showed sporadic sectorization and mycelia from the sectored area stably maintained the sectored phenotype. These results suggest that the unique sectorization is CWI pathway-specific, though the components in the same CWI pathway have common and specific functions.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.29 no.10 s.241
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• pp.1361-1368
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• 2005

The structural and leakage integrity of steam generator tubes should be sustained all postulated loads with appropriate margin even if a crack is present. During the past three decades, for effective integrity evaluation, several limit load solutions have been used world-widely. However, to predict accurately load carrying capacities of specific components under different conditions, the solutions have to be modified by using lots of experimental data. The purpose of this paper is to propose a new burst pressure estimation scheme based on fracture mechanics analyses for steam generator tube with an axial or circumferential through-wall crack. A series of three dimensional elastic-plastic finite element analyses were carried out and, then, closed-form estimation equations with respect to both J-integral and crack opening displacement were derived through reference stress method. The developed engineering equations were utilized for structural integrity evaluation and the resulting data were compared to the corresponding ones fiom experiments as well as limit load solutions. Thereafter, since the effectiveness was proven by promising results, it is believed that the proposed estimation scheme can be used as an efficient tool for integrity evaluation of cracked steam generator tubes.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.2
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• pp.33-37
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• 2006

Since 1993, the civil aviation community through RTCA (Radio Technical Commission for Aeronautics) and the ICAO (International Civil Air Navigation Organization) have been working on the definition of GNSS augmentation systems that will provide improved levels of accuracy and integrity. These augmentation systems have been classified into three distinct groups: Aircraft Based Augmentation Systems (ABAS), Space Based Augmentation Systems (SBAS) and Ground Based Augmentation Systems (GBAS). The last one is an implemented system to support Air Navigation in CAT-I approaching operation. It consists of three primary subsystems: the GNSS Satellite subsystem that produces the ranging signals and navigation messages; the GBAS ground subsystem, which uses two or more GNSS receivers. It collects pseudo ranges for all GNSS satellites in view and computes and broadcasts differential corrections and integrity-related information; the Aircraft subsystem. Within the area of coverage of the ground station, aircraft subsystems may use the broadcast corrections to compute their own measurements in line with the differential principle. After selection of the desired FAS for the landing runway, the differentially corrected position is used to generate navigation guidance signals. Those are lateral and vertical deviations as well as distance to the threshold crossing point of the selected FAS and integrity flags. The Department of Applied Science in Naples has create for its study a virtual GBAS Ground station. Starting from three GPS double frequency receivers, we collect data of 24h measures session and in post processing we generate the GC (GBAS Correction). For this goal we use the software Pegasus V4.1 developed from EUROCONTROL. Generating the GC we have the possibility to study and monitor GBAS performance and integrity starting from a virtual functional architecture. The latter allows us to collect data without the necessity to found us authorization for the access to restricted area in airport where there is one GBAS installation.

• Advances in Energy Research
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• v.6 no.1
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• pp.75-90
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• 2019

Anthropogenic greenhouse gas emissions are rising rapidly despite efforts to curb release of such gases. One long term potential solution to offset these destructive emissions is the capture and storage of carbon dioxide. Partially depleted hydrocarbon reservoirs are attractive targets for permanent carbon dioxide disposal due to proven storage capacity and seal integrity, existing infrastructure. Optimum well completion design in depleted reservoirs requires understanding of prominent geomechanics issues with regard to rock-fluid interaction effects. Geomechanics plays a crucial role in the selection, design and operation of a storage facility and can improve the engineering performance, maintain safety and minimize environmental impact. In this paper, an integrated geomechanics workflow to evaluate reservoir caprock integrity is presented. This method integrates a reservoir simulation that typically computes variation in the reservoir pressure and temperature with geomechanical simulation which calculates variation in stresses. Coupling between these simulation modules is performed iteratively which in each simulation cycle, time dependent reservoir pressure and temperature obtained from three dimensional compositional reservoir models in ECLIPSE were transferred into finite element reservoir geomechanical models in ABAQUS and new porosity and permeability are obtained using volumetric strains for the next analysis step. Finally, efficiency of this approach is demonstrated through a case study of oil production and subsequent carbon storage in an oil reservoir. The methodology and overall workflow presented in this paper are expected to assist engineers with geomechanical assessments for reservoir optimum production and gas injection design for both natural gas and carbon dioxide storage in depleted reservoirs.

• Journal of Korea Multimedia Society
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• v.24 no.8
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• pp.1044-1058
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• 2021

Tempered electronic contents have multiplied in last few years, thanks to the emergence of sophisticated artificial intelligence(AI) algorithms. Deepfakes (fake footage, photos, speech, and videos) can be a frightening and destructive phenomenon that has the capacity to distort the facts and hamper reputation by presenting a fake reality. Evidence of ownership or authentication of digital material is crucial for combating the fabricated content influx we are facing today. Current solutions lack the capacity to track digital media's history and provenance. Due to the rise of misrepresentation created by technologies like deepfake, detection algorithms are required to verify the integrity of digital content. Many real-world scenarios have been claimed to benefit from blockchain's authentication capabilities. Despite the scattered efforts surrounding such remedies, relatively little research has been undertaken to discover where blockchain technology can be used to tackle the deepfake problem. Latest blockchain based innovations such as Smart Contract, Hyperledger fabric can play a vital role against the manipulation of digital content. The goal of this paper is to summarize and discuss the ongoing researches related to blockchain's capabilities to protect digital content authentication. We have also suggested a blockchain (smart contract) dependent framework that can keep the data integrity of original content and thus prevent deepfake. This study also aims at discussing how blockchain technology can be used more effectively in deepfake prevention as well as highlight the current state of deepfake video detection research, including the generating process, various detection algorithms, and existing benchmarks.

• Animal Bioscience
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• v.35 no.2
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• pp.166-176
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• 2022

Objective: Sperm is particularly susceptible to reactive oxygen species (ROS) stress. Glutathione (GSH) is an endogenous antioxidant that regulates sperm redox homeostasis. However, it is not clear whether boar sperm could utilize cysteine for synthesis GSH to protect sperm quality from ROS damage. Therefore, the present study was undertaken to elucidate the mechanism of how cysteine is involved in protecting boar sperm quality during liquid storage. Methods: Sperm motility, membrane integrity, lipid peroxidation, 4-hydroxyIlonenal (4-HNE) modifications, mitochondrial membrane potential, as well as the levels of ROS, GSH, and, ATP were evaluated. Moreover, the enzymes (GCLC: glutamate cysteine ligase; GSS: glutathione synthetase) that are involved in glutathione synthesis from cysteine precursor were detected by western blotting. Results: Compared to the control, addition of 1.25 mM cysteine to the liquid storage significantly increased boar sperm progressive motility, straight-line velocity, curvilinear velocity, beat-cross frequency, membrane integrity, mitochondrial membrane potential, ATP level, acrosome integrity, activities of superoxide dismutase and catalase, and GSH level, while reducing the ROS level, lipid peroxidation and 4-HNE modifications. It was also observed that the GCLC and GSS were expressed in boar sperm. Interestingly, when we used menadione to induce sperm with ROS stress, the menadione associated damages were observed to be reduced by the cysteine supplementation. Moreover, compared to the cysteine treatment, the γ-glutamylcysteine synthetase (γ-GCS) activity, GSH level, mitochondrial membrane potential, ATP level, membrane integrity and progressive motility in boar sperm were decreased by supplementing with an inhibitor of GSH synthesis, buthionine sulfoximine. Conclusion: These data suggest that boar sperm could biosynthesize the GSH from cysteine in vitro. Therefore, during storage, addition of cysteine improves boar sperm quality via enhancing the GSH synthesis to resist ROS stress.

• Animal Bioscience
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• v.37 no.5
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• pp.852-861
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• 2024

Objective: The present study aimed to investigate the effect of β-nicotinamide mononucleotide (NMN) supplementation on ram sperm quality during storage at 4℃ in vitro. Methods: Tris-citric acid-glucose solution containing different doses of NMN (0, 30, 60, 90, and 120 µM) was used to dilute semen collected from rams and it was stored at 4℃. Sperm motility, plasma membrane integrity as well as acrosome integrity were evaluated at 0, 24, and 48 h time points after storage at 4℃. In addition, sperm mitochondrial activity, lipid peroxidation (LPO), malondialdehyde (MDA) content, reactive oxygen species (ROS) content, glutathione (GSH) content, superoxide dismutase (SOD) activity, and apoptosis were measured at 48 h time point after storage at 4℃. Results: Results demonstrate that the values obtained for sperm motility, acrosome integrity, and plasma membrane integrity in the NMN treatments were significantly higher than control (p<0.05). The addition of 60 µM NMN significantly improved ram sperm mitochondrial activity and reduced LPO, MDA content, and ROS content compared to control (p<0.05). Interestingly, sperm GSH content and SOD activity for the 60 µM NMN treatment were much higher than those observed for control. NMN treatment also decreased the level of Cleaved-Caspase 3, Cleaved-Caspase 9, and Bax while increasing Bcl-2 level in sperm at 48 h time point after storage at 4℃. Conclusion: Ram sperm quality can be maintained during storage at 4℃ with the addition of NMN at 60 µM to the semen extender. NMN also reduces oxidative stress and apoptosis. Overall, these findings suggest that NMN is efficient in improving the viability of ram sperm during storage at 4℃ in vitro.

• Journal of the Korean Operations Research and Management Science Society
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• v.32 no.2
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• pp.149-162
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• 2007

In the era of the internet and ubiquitous computing, IS users are still facing a variety of threats. Therefore, a need of more tightened information security service increases unprecedentedly. In this sense, this study is aimed at proposing a new research model in which IT assets (i.e., network, system, and information influence) Security and Information Security Service (i.e., confidentiality, integrity, nonrepudiation, authentication) affect information security quality positively, leading to users' satisfaction eventually. To prove the validity of the proposed research model, PLS analysis is applied with valid 177 questionnaires. Results reveal that both IT assets Security and Information Security Service influence informations security qualify positively, and user satisfaction as well. From the results, it can be concluded that Korean government's recent orchestrated efforts to boost the IT assets Security and Information Security Service helped great improve the information security quality and user satisfaction.

• International Journal of Contents
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• v.3 no.4
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• pp.1-7
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• 2007

There has been increased interest in wireless sensors in the last few years. This paper provides insight into the properties that make these sensors so attractive, specifically considering their efficiency, data reliability, and the ability to verify the data generated. Some advantages a wireless network presents over traditional information sensing are discussed as well. The paper considers how the environmental field can benefit from using these networks. Some of the possible challenges this industry will face in adopting this new method of data sampling and collection are also considered. A project we conducted raised concern over measures needed for the integrity of the communication system to be maintained, thus ensuring the integrity of the data being collected. From results of an experimental project conducted in York University, the reliability and usefulness of a sensor network is discussed.

• Journal of Korean Academy of Nursing
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• v.31 no.6
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• pp.1109-1118
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• 2001

The purpose of this study was to analyze the appraising frame and the accreditation system of universities in both Korea and the United Sates, as well as developing a standard plan to appraise 4 year nursing education institutions in Korea. Research design of this study was descriptive comparative research. The results of the research can be divided into six fields, educational purposes, educational courses, students, faculty, facility .equipments and administration.finance in Korea. Then the results were further categorized into seven fields : mission and governance, faculty, students, curriculum and instruction, resources, educational effectiveness and integrity. Thus Korean appraising frames were suggested to have eight fields of standard. The fields are philosophy and purposes of educational institution, educational courses and instruction, students, faculty, facility .equipments, adminstration.finance, educational effectiveness and integrity.