Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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β-Nicotinamide mononucleotide improves chilled ram sperm quality in vitro by reducing oxidative stress damage

  • Zhendong Zhu (College of Animal Science and Technology, Qingdao Agricultural University) ;
  • Haolong Zhao (College of Animal Science and Technology, Qingdao Agricultural University) ;
  • Qitai Yang (College of Animal Science and Technology, Qingdao Agricultural University) ;
  • Yajing Li (College of Animal Science and Technology, Qingdao Agricultural University) ;
  • Ruyuan Wang (College of Animal Science and Technology, Qingdao Agricultural University) ;
  • Adedeji Olufemi Adetunji (Department of Agriculture, University of Arkansas at Pine Bluff) ;
  • Lingjiang Min (College of Animal Science and Technology, Qingdao Agricultural University)
  • Received : 2023.09.23
  • Accepted : 2024.01.13
  • Published : 2024.05.01
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Abstract

Objective: The present study aimed to investigate the effect of β-nicotinamide mononucleotide (NMN) supplementation on ram sperm quality during storage at 4℃ in vitro. Methods: Tris-citric acid-glucose solution containing different doses of NMN (0, 30, 60, 90, and 120 µM) was used to dilute semen collected from rams and it was stored at 4℃. Sperm motility, plasma membrane integrity as well as acrosome integrity were evaluated at 0, 24, and 48 h time points after storage at 4℃. In addition, sperm mitochondrial activity, lipid peroxidation (LPO), malondialdehyde (MDA) content, reactive oxygen species (ROS) content, glutathione (GSH) content, superoxide dismutase (SOD) activity, and apoptosis were measured at 48 h time point after storage at 4℃. Results: Results demonstrate that the values obtained for sperm motility, acrosome integrity, and plasma membrane integrity in the NMN treatments were significantly higher than control (p<0.05). The addition of 60 µM NMN significantly improved ram sperm mitochondrial activity and reduced LPO, MDA content, and ROS content compared to control (p<0.05). Interestingly, sperm GSH content and SOD activity for the 60 µM NMN treatment were much higher than those observed for control. NMN treatment also decreased the level of Cleaved-Caspase 3, Cleaved-Caspase 9, and Bax while increasing Bcl-2 level in sperm at 48 h time point after storage at 4℃. Conclusion: Ram sperm quality can be maintained during storage at 4℃ with the addition of NMN at 60 µM to the semen extender. NMN also reduces oxidative stress and apoptosis. Overall, these findings suggest that NMN is efficient in improving the viability of ram sperm during storage at 4℃ in vitro.

Keywords

Acknowledgement

This work was supported in part by the Young Innovation Team Plan Program of Higher Education of Shandong Province Project for Z Zhu (2022KJ170), Technology System of Modern Agricultural Industry in Shandong Province (SDAIT-10-08) for L Min, and the Start-up Fund for High-level Talents of Qingdao Agricultural University for Z Zhu (1121010).

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