• Journal of Ginseng Research
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• 제38권4호
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• pp.289-292
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• 2014

Ginseng leaf/stem extract produced by subcritical water extraction at high temperature ($190^{\circ}C$) posses higher cytotoxic activity against human cancer cell lines than ethanol extract. Subcritical water extraction can be a great candidate for extraction of functional substance from ginseng leaves/stems.

• 생약학회지
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• 제39권3호
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• pp.255-259
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• 2008

This study was investigated antioxidant and anticancer activity of water, methanol extract from upper and root part of Taraxacum mongolicum H. Total phenolic compound contents of methanol and water extracts from upper part were $51.95{\pm}0.18$ mg/g and $48.16{\pm}0.89$ mg/g respectively, and total flavonoid compound contents were estimated as $20.57{\pm}1.12$ mg/g in methanol extract and $6.55{\pm}1.20$ mg/g in water extract. $EC_{50}$ values for DPPH radical scavenging activity of methanol and water extract from upper part were $138.47{\pm}3.78{\mu}g/mL$ and $204.38{\pm}5.32{\mu}g/mL$, and methanol and water extracts from root part were as $512{\pm}8.11{\mu}g/mL$ in methanol extract and $1315.05{\pm}11.98{\mu}g/mL$ in water extract. Reducing power and hydroxyl radical $({\bullet}OH)$ scavenging activity estimated that methanol extract of each part were higher than water extracts. The cell viability showed that the methanol extract from upper part had a cytotoxicity in the growth of colon carcinoma cell (44.58%). Both water extract $(51.97{\pm}11.43%)$ from upper part and methanol $(53.46{\pm}19.77%)$, water $(52.79{\pm}13.53%)$ extracts from root part had quite higher cytotoxicity than that of methanol extract $(88.25{\pm}2.02%)$ from upper part. Based on the results, It was suggested that the methanol extract of Taraxacum mongolicum H. were potential materials for use as functional food and medicine.

• 대한본초학회지
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• 제23권3호
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• pp.41-51
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• 2008

Objectives : This study was conducted to evaluate the antioxidative and anticancer activity of extracts from Scutellaria baicalensis, Zizyphus jujuba and Atractylodes macrocephala. Methods : Three kinds of medicinal herbs were extracted with distilled water and 70% ethanol, and the extracts were tested for their antioxidant activity and growth inhibition activity against cancer cells. Results : Electron donating abilities (EDA) of the water and ethanol extract from Zizyphus jujuba and Atractylodes macrocephala were over than 87% at 1,000 ${\mu}g/mL$, respectively. The highest nitrite scavenging abilities (NSA) of the water and ethanol extracts from Scutellaria baicalensis were 68.9 and 79.2% at 1,000 ${\mu}g/mL$, respectively. Atractylodes macrocephala extracts had shown the highest growth inhibition activity against both of MDA and A549 cells and the water extract had higher activity than ethanol extract. Conclusions : These results suggest that the extracts of medicinal herbs used for this experiment had specific biological activity and they can be used as natural antioxidant to prevent oxidative damage in normal cells.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권2호
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• pp.76-84
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• 2002

On-line conversion estimation of enzymatic esterification reactions in solvent-free media was investigated. In principle, conversion to ester can be determined from the amount of water produced by the reaction, because water is formed as a by-product in a stoichiometric manner. In this study, we estimated the water production rate only from some measurements of relative humidity and water balances without using any analytical methods. In order to test the performance of the on-line conversion estimation, the lipase-catalyzed esterification of n-capric acid and n-decal alcohol in solvent-free media was performed whilst controlling water activity at various values. The reaction conversions estimated on-line were similar to those determined by offline gas chromatographic analysis. However, when the water activity was controlled at higher values, discrepancies between the estimated conversion values and the measured values became significant. The deviation was found to be due to the inaccurate measurement of the water content in the reaction medium during the initial stages of the reaction. Using a digital filter, we were able to improve the accuracy of the on-line conversion estimation method considerably. Despite the simplicity of this method, the on-line estimated conversions were in good agreement with the off-line measured values.

• 한방안이비인후피부과학회지
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• 제16권2호
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• pp.96-118
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• 2003

The effect of Glycyrrhizae Radix water extract, known as depigmenting agent, on melanin biosynthesis was investigated in cellular level by using B16 mouse melanoma cells. The inhibitory effect of Glycyrrhizae Radix water extract on melanogenesis was determined by mushroom tyrosinase assay traditionally using in vitro screening test. To determine whether Glycyrrhizae Radix water extract suppress melanin synthesis in cellular level, B16 mouse melanoma cells were cultured in the presence of different concentrations of Glycyrrhizae Radix water extract. Effects on cell proliferation, melanin biosynthesis, tyrosinase activity, DOPAchrome tautomerase activity, and expression level of mRNA for tyrosinase were examined. The maximum concentration of Glycyrrhizae Radix water extract that was not inhibitory to growth of the cells was 2 mgml. At that concentration, melanin synthesis was significantly inhibited without cytotoxicity after 5 days, compared with untreated cells. The treatment with Glycyrrhizae Radix water extract reduced tyrosinase and DOPAchrome tautomerase activity in a dose-dependent manner. However, the treatment with Glycyrrhizae Radix water extract did not affect significantly mRNA levels for tyrosinase. These results suggest that the inhibitory effect of Glycyrrhizae Radix water extract on melanogenesis is correlated with the suppression of tyrosinase and DOPAchrome tautomerase activity more than altering mRNA levels of tyrosinase.

• International Journal of Oral Biology
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• 제38권2호
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• pp.81-85
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• 2013

There are estimated to be about 700 species of bacteria in the oral cavity. Based on epidemiological investigations, some of these strains have been proposed as the pathogens responsible for oral diseases such as dental caries, gingivitis and periodontitis. Since electrolyzed hydrogen-rich water has been shown to have beneficial effects on human immunity, its use has increased. In our study, the antibacterial activity of hydrogen-rich water for oralagainst bacteria associated with oral disease was evaluated. The bacterial strains Streptococcus mutans, Fusobacterium nucleatum, Porphyromonas gingivalis and Tannerella forsythia were cultured in specific growth medium. S. mutans, F. nucleatum and P. gingivalis were soaked to thein both hydrogen water and tap water for 30 sec and then inoculated onto mitis-salivarius agar and brain heart infusion agar including supplemented withvitamin K and hemin, respectively. The numbers of bacterial colonies were then measured after cultivation for 48 hours. In the case of T. forsythia, which does not grow well on agar plates, inoculations into modified new oral spirochete (NOS) broth were performed and growth curve analysis was undertaken every day with a spectrophotometer. Hydrogen water showed antibacterial activity against all four bacterial strains in comparison with tap-water. We conclude from this that hydrogen water may have a positive impact on oral hygiene by helping to remove cariogenic bacteria and periodontopathogens.

• 생명과학회지
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• 제10권5호
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• pp.524-528
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• 2000

택사(Alismatis Rhizoma)는 중국에서 많이 생산되고, 우리나라에서도 그 생산량의 50%이상이 전남 순천지방에서 생산되고 있는데, 옛부터 생약으로 많이 사용되어 왔으며, 최근 택사분말은 무좀에 도포약으로 쓰이고 있어 택사에는 다양한 생리활성이 있는 것으로 생각된다. 따라서 본 연구에서는 추출용매에 따른 택사 추출물에 대한 항균 및 항산화성에 대하여 조사하였다. Paper disc법에 의한 택사 추출물의 항균 test결과 물추출물에서는 거의 모든 시험균주에서 항균활성이 나타났으나, 메탄올 및 에탄올 추출에서는 그 활성이 작게 나타났으며, 시험균주중 Vibrio parahaemolyticus균에 가장 강한 항균활성을 나타내었다. Linoleic acid에 대한 항산화력의 조사에서 택사 물추출물 100$\mu{l}$첨가시 메탄올 및 에탄올 추출물은 항균효과 나타나지 않았고, 물추출물은 효과가 나타났으나 0.1% BHT첨가시 보다는 그 효과가 적었다.

• 한국생활과학회지
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• 제22권6호
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• pp.687-699
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• 2013

This study was carried out to investigate the antifungal activity and house dust mite repellent effect of cotton and silk fabrics dyed with Juniperus chinensis heartwood extracts by hot-water and methanol. The chemical composition of Juniperus chinensis heartwood extracts by hot-water and methanol was analyzed by GC-MS. In the case of antifungal activity, dyed fabrics with hot water extract had no effect against chaetomium globosum fungus. But showing no growth in fabrics dyed with methanol extract, dyed fabrics with methanol extract showed very excellent antifungal activity. Silk fabric dyed with methanol extract showed excellent house dust mite repellent effect of 94.3%~96.0% against dermatophagodes farinae. The composition of Juniperus chinensis heartwood extracts from hot-water and methanol was different. There were no terpene and its analogue peaks in hot-water extract. However there were terpene and its analogue peaks in methanol extract. From these GC-MS results, terpene was identified in methanol extract of Juniperus chinensis heartwood. Efficient ingredient of antifungal activity and house dust mite repellent effect was assumed terpene.

• 한국식품영양학회지
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• 제35권2호
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• pp.96-105
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• 2022

This study compared and analyzed the antioxidant effect of Doenjang prepared from vegetable water, and explored the optimal addition ratio of vegetables of 5 kinds and the possibility of application to Doenjang. The sample is three kinds of vegetable water (VW1, VW2, VW3) prepared by adding different ratios of radish, carrot, green onion, onion and shiitake mushroom and Denjang prepared using it. Doenjang was aged and fermented at about 40℃ for 40 days, and then separated and used only solids. The content of their antioxidant compounds was measured the content of total phenolic acid contents and total flavonoid contents. In addition, the antioxidant effect was measured by electron donating activity, SOD-like activity, ABTs radical scavenging activity and reducing power. The total phenolic acid contents and total flavonoid contents were high at VW3 and that Doenjang made with VW3. Electron donating activity and SOD-liked activity were high at VW2 and Doenjang made with VW2. ABTs radical scavenging activity was high in Doenjang made of VW3, and Reducing power was high in VW3. Therefore, if Doenjang is prepared with vegetable water prepared by properly mixing 5 types of vegetables, the possibility of developing Doenjang with high antioxidant effect was suggested.

• 한국식품저장유통학회지
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• 제6권1호
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• pp.99-103
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• 1999

Corni Fructus extracts were investigated for antimicrobial and antioxidative activities. In the methanol, ethanol, water extracts from Corni Fructus, antimicrobial activity of the water extract was stronger than the others. Antimicrobial activities were strong against microorganisms such as Streptococcus mutans, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhimurium, Escherichia coli and Escherichia coli O-157, the activity against Escherichia coli O-157 was the strongest of all the microorganisms. The growth of all the microorganisms were completely inhibited to 48 hours in the 150${\mu}\ell$ per $m\ell$ of culture media. the shape of E. coli O-157 treated with Corni Fructus water extract was expended and its cellular surface was collapsed severely. When Corni Fructus water extract of 200${\mu}\ell$ was treated, hydrogen donating effect was more than 90%, and antioxidative activity using linoleic acid was similar to 0.1% BHT at the concentration of 50${\mu}\ell$.