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The Genetic Variability and Relationships of Japanese and Foreign Chickens Assessed by Microsatellite DNA Profiling

  • Osman, S.A.M.;Sekino, M.;Nishihata, A.;Kobayashi, Y.;Takenaka, W.;Kinoshita, K.;Kuwayama, T.;Nishibori, M.;Yamamoto, Y.;Tsudzuki, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.10
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    • pp.1369-1378
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    • 2006
  • This is the first study in which genetic variability and relationships of a large number of Japanese chicken breeds were revealed along with those of several foreign breeds by using microsatellite DNA polymorphisms. Twenty-eight breeds (34 populations) of native Japanese chickens and seven foreign breeds or varieties were analyzed. The mean number of alleles per locus, the proportion of the polymorphic loci, and the expected average heterozygosity ranged from 1.75 to 4.70, from 0.55 to 1.00, and from 0.21 to 0.67, respectively. Microsatellite alleles being unique to a particular population were detected in some populations. The $D_A$ genetic distance between populations was obtained from allele frequency for every pair of the populations to construct a neighbor-joining tree. According to the phylogenetic tree, excluding a few exceptions, native Japanese chicken breeds and foreign breeds were clearly separated from each other. Furthermore, the tree topology divided native Japanese chickens into four main classes, which was almost in accordance with the classification based on body morphology; that is, (1) Cochin type, (2) Malay type, (3) layer type, and (4) intermediate type between Malay and layer types. This is the first finding for native Japanese chickens.

Influences of Nd-Fe-B Magnets on the Magnetic Anisotropy Direction of Permalloy Thin Films Fabricated by rf Magnetron Sputtering (Rf 마그네트론 스퍼터링으로 제조된 퍼멀로이 박막의 자기이방성 조절을 위한 NdFeB 영구자석의 영향 및 자기특성 해석)

  • Lee, Y.H.;Kim, K.H.;Kim, J.
    • Journal of the Korean Magnetics Society
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    • v.12 no.2
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    • pp.51-56
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    • 2002
  • Permalloy thin films fabricated by rf magnetron sputtering showed the excellent magnetic properties, i.e., an effective permeability of over 2000 at 1$\mu\textrm{m}$ thick up to 10 MHz, a saturation magnetization of 10∼12 kG, a coercive force of 0.2∼1 Oe, resistivity (p) is 20 ${\mu}$$\Omega$cm. In order to control the magnetic anisotropy direction of the films in a wafer scale, two parallel Nd-Fe-B permenant mangnets were used to provide the magnetic field during the sputtering process. As a result, the anisotropy direction was successfully controlled when the two magnets were seperated with a distance of 70 mm. 3D simmulation of the magnteic fields around the wafer during sputtering were in accord with the above result.

Electrical Resistivity Survey in the Eon-Yang Fault Area, Southeastern Korean Peninsula (경상분지(慶尙盆地) 언양단층(彦陽斷層) 지역(地域)에 대(對)한 전기비저항(電氣比抵抗) 탐사연구(探査硏究))

  • Kim, In-Soo;Kim, Jong-Yeol
    • Economic and Environmental Geology
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    • v.16 no.1
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    • pp.11-18
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    • 1983
  • Gyeongsang Basin in the southeastern part of the Korean peninsular is characterized by many fault systems. To decipher the geotectonical evolution of the Korean peninsular and marginal basins in her adjacent areas it is prerequisite to understand the spatial distribution pattern and mutual relationships of these fault systems. Because of difficulties in finding any criterion to recognize the faults in field, their extension and mutual relationships in ages are not very clear yet. As an attempt to find geophysical criteria to recognize the fault, geoelectrical resistivity survey was carried out in this study. With the Wenner configuration four resistivity soundings and twenty seven resistivity profilings were done. The electrode distance used was up to 50m. From the results of the resistivity soundings and boring data of earlier groundwater investigations the depth of alluvial and weathered zone was established to be at most 20m in the study area. In the resistivity profiling low resistivity anomaly zones are detected on every traverse, which are interpreted as caused by fractures, fault clays and mylonites in the fault zone. The width of the fault zone amounts to 0.3-1km. By correlating and connecting the negative anomaly zones from traverse to traverse one can determine the trend of th of the faultzone and therefore that of fault itself. The recognized fault trend in this way was $N15^{\circ}-20^{\circ}E$ and this coincides with the direction of the inferred fault line from earlier geological surface mapping. With the help of this characteristical negative anomaly the existance of another $N80^{\circ}W$ trending fault was estabished. This study has shown that geoelectrical resistivity survey can be applied successfully to the problem of tracing fault line insofar as a fault zone has been developed along fault line.

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Fabrication Of Ultraviolet LED Light Source Module Of Current Limiting Diode Circuit By Using Flip Chip Micro Soldering (마이크로솔더링을 이용한 정전류다이오드 회로 자외선 LED 광원모듈 제작)

  • Park, Jong-Min;Yu, Soon Jae;Kawan, Anil
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.29 no.4
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    • pp.237-240
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    • 2016
  • The improvement of irradiation intensity and irradiation uniformity is essential for large area and high power UVA light source application. In this study, large number of chips bonded by micro soldering technique were driven by low current, and current limiting diodes were configured to supply constant current to parallel circuits consisting of large number of series strings. The dimension of light source module circuit board was $350{\times}90mm^2$ and 16,650 numbers of 385 nm flip chip LEDs were used with a configuration of 90 parallel and 185 series strings. The space between LEDs in parallel and series strings were maintained at 1.9 mm and 1.0 mm distance, respectively. The size of the flip chip was $750{\times}750{\mu}m^2$ were used with contact pads of $260{\times}669{\mu}m^2$ size, and SAC (96.5 Sn/3.0 Ag/0.5 Cu) solder was used for flip chip bonding. The fabricated light source module with 7.5 m A supply current showed temperature rise of $66^{\circ}C$, whereas irradiation was measured to be $300mW/cm^2$. Inaddition, 0.23% variation of the constant current in each series string was demonstrated.

Effects of geometric conditions of blade on Performance of Axial Pan (익형의 기하학적 조건에 따른 축류팬의 성능에 관한 연구)

  • Ahn E. Y.;Kim J. W.;Jeongng E. J.
    • 한국전산유체공학회:학술대회논문집
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    • 2005.04a
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    • pp.25-29
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    • 2005
  • Axial fan is used for the supplement of large amount of flows. Axial blowers show relatively high efficiency of the system. The present model of axial fan is for cooling a condenser in an air-conditioning unit that exhibits tendency toward compact size. In order to realize the compact model, the width of an axial blade should be cut down in axial distance. Main interest lies on the performance of the axial blowing system with blades having shorter chord length. One of the important design parameters for axial fan is the shape of the blades of it. Design of blades includes the cross-sectional shape and its dimension, including the chord length. We consider two types of blades; one is NACA airfoil with normal chord length and the other is with shortening chord length by $10\%$ of normal airfoil. Axial blower with the modified blades is essential for the compact model of an air-conditioner. The other design parameters are same in the two cases. Using a wind tunnel follows ASHRAE standards carries out evaluation of performance of the system. Detail of flows around the blades is prepared by velocity measurements using PIV. According to performance estimation, the axial blower with short chord blade show quite close to the performance results, including flow rate and pressure rise, of the standard one. The reason of the two similar results is that the flowpatterns depend on Reynolds number based on the chord length of a blade. In this investigation, the critical chord length is found, in which the flows near the airfoil are so unstable and the performance of the system is decreased. A series of figures is for the detail information on the flow.

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A Comparison of Two Kinds of Markers Applied in Analysis of Genetic Diversity in Sheep and Goat Populations

  • Yang, Z.P.;Chang, H.;Sun, W.;Gen, R.Q.;Mao, Y.J.;Tsunoda, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.7
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    • pp.892-896
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    • 2004
  • A genetic examination using 14 structural loci and 7 microsatellite markers was carried out among random samples of Hu sheep (Hu), Tong sheep (Tong) and Yantse River Delta White goat (YRD); The mean heterozygosity (H), mean polymorphism information contents (PIC) and mean effective numbers of alleles (Ne) calculated based on the data from the above two types of genetic markers were compared. The standard genetic distances among the three populations based on two types of gene frequencies were calculated and compared. The results show that the mean heterozygosity (H), mean polymorphism information contents (PIC) and mean effective numbers of alleles (Ne) based on 7 microsatellite markers are greater than those based on the structural loci. The standard genetic distances based on structural loci among the three populations are: 0.0268-0.2487, the standard genetic distances based on microsatellite markers are: 0.2321-1.2313. The study indicates that structural and microsatellite markers reflect the genetic variation of the three populations consistently: Tong>Hu>YRD. The differentiation between related species or interpopulations can be expressed more effectively by microsatellite markers than structural markers. Oar FCB11, MAF33, Oar AE101, Oar FCB128 and OarFCB304 can be used as representative loci for research on genetic differentiation between sheep and goat.

Discrimination of Korean Native Chicken Lines Using Fifteen Selected Microsatellite Markers

  • Seo, D.W.;Hoque, M.R.;Choi, N.R.;Sultana, H.;Park, H.B.;Heo, K.N.;Kang, B.S.;Lim, H.T.;Lee, S.H.;Jo, C.;Lee, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.3
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    • pp.316-322
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    • 2013
  • In order to evaluate the genetic diversity and discrimination among five Korean native chicken lines, a total of 86 individuals were genotyped using 150 microsatellite (MS) markers, and 15 highly polymorphic MS markers were selected. Based on the highest value of the number of alleles, the expected heterozygosity (He) and polymorphic information content (PIC) for the selected markers ranged from 6 to 12, 0.466 to 0.852, 0.709 to 0.882 and 0.648 to 0.865, respectively. Using these markers, the calculated genetic distance (Fst), the heterozygote deficit among chicken lines (Fit) and the heterozygote deficit within chicken line (Fis) values ranged from 0.0309 to 0.2473, 0.0013 to 0.4513 and -0.1002 to 0.271, respectively. The expected probability of identity values in random individuals (PI), random half-sib ($PI_{half-sibs}$) and random sibs ($PI_{sibs}$) were estimated at $7.98{\times}10^{-29}$, $2.88{\times}10^{-20}$ and $1.25{\times}10^{-08}$, respectively, indicating that these markers can be used for traceability systems in Korean native chickens. The unrooted phylogenetic neighbor-joining (NJ) tree was constructed using 15 MS markers that clearly differentiated among the five native chicken lines. Also, the structure was estimated by the individual clustering with the K value of 5. The selected 15 MS markers were found to be useful for the conservation, breeding plan, and traceability system in Korean native chickens.

The Effect of Cross Beam on the strength and Stiffness of the Frame in Shuttle Car for LMTT (LMTT용 셔틀 카의 프레임 강도 및 강성에 미치는 크로스 빔의 영향)

  • Lim J. H.;Han G. J.;Lee K. S.;Han D. S.;Shim J. J.;Lee S. W.;Jeon Y. H.
    • Journal of Navigation and Port Research
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    • v.29 no.1 s.97
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    • pp.77-82
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    • 2005
  • According as the quantity of goods transported by ship is increasing, a port environment is rapidly changing To meet this situation successfully, the development of the next generation port loading and unloading system(LMTT) is studied A Frame of shuttle car for LMTT(Linear Motor-based Transfer Technology) consists of three parts which are outer beam, inner beam and cross beam In this study, we carried out the finite element analysis for the effect of cross beam on the strength and stiffness of the frame according to the number of cross beam, loading position of container, the distance ratio of inner beam from center. The result is as follow ; When the load is applied on outer beam and inner beam concurrently and the number of cross beam is 5, that is the optimum condition in frame design.

Analysis of cellular fatty acid methyl esters (FAMEs) for the identification of leuconostoc strains isolated from kimchi

  • Lee, Jung-Sook;Chun, Chang-Ouk;Kim, Hong-Joong;Joo, Yun-Jung;Lee, Hun-Joo;Park, Chan-Sum;Park, Yong-Ha;Mheen, Tae-Ick
    • Journal of Microbiology
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    • v.34 no.3
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    • pp.225-228
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    • 1996
  • The cellular fatty acid methyl esters (FAMEs) analysis data obtained for clusters defined at a Euclidian distance of 17.5, in the classification of lactic acid bacteria isolated from kimchi, described by Lee et al. (4), was used for the identification of 79 Leuconostoc isolates. The test strains were isolated using a selective isolation medium specific for the genus Leuconostoc. These strains were then characterized according to their fatty acid profiles. The results show that all seventy nine test strains were identified to the known Leuconostoc clusters B, C, and D. Cluster B had the highest relative amount of the saturated fatty acid 16 : 0. The saturated fatty acid 16 : 0 and summed feature 9 were found as a major components in cluster C, which had a higher level of summed feature 9 than cluster B. Cluster D is characterized by the highest relative amount of the unsaturated fatty acid 18 : 1 w9c. It is suggested that FAMEs analysis can be successfully applied in the identification of lactic acid bacteria isolated from kimchi.

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Obstacle avoidance of Mobile Robot with Virtual Impedance (가상임피던스를 이용한 원격 이동로봇의 장애물회피)

  • Jin, Tae-Seok
    • Journal of the Korean Institute of Intelligent Systems
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    • v.19 no.4
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    • pp.451-456
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    • 2009
  • In this paper, a virtual force is generated and fed back to the operator to make the teleoperation more reliable, which reflects the relationship between a slave robot and an uncertain remote environment as a form of an impedance. In general, for the teleoperation, the teleoperated mobile robot takes pictures of the remote environment and sends the visual information back to the operator over the Internet. Because of the limitations of communication bandwidth and narrow view-angles of camera, it is not possible to watch certain regions, for examples, the shadow and curved areas. To overcome this problem, a virtual force is generated according to both the distance between the obstacle and the robot and the approaching velocity of the obstacle w.r.t the collision vector based on the ultrasonic sensor data. This virtual force is transferred back to the master (two degrees of freedom joystick) over the Internet to enable a human operator to estimate the position of obstacle at the remote site. By holding this master, in spite of limited visual information, the operator can feel the spatial sense against the remote environment. It is demonstrated by experiments that this collision vector based haptic reflection improves the performance of teleoperated mobile robot significantly.