• Applied Microscopy
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• 제43권4호
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• pp.146-154
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• 2013

Capillaria hepatica (syn. Calodium hepatica) is a parasite found mainly in rodent liver. But, it has also been found in a wide variety of mammals, including humans. This worm is unique as it is the only nematode parasite that is embedded in the liver parenchyma of the host even during the adult stage of the life cycle. They produce eggs that elicit a marked granulomatous reaction that eventually destroys the worms. Fibrosis and lymphoplasmacytic inflammatory infiltration are often observed around adult nematodes embedded in the liver parenchyma of the host. For this reason, complete isolation of this slender worm and observation of the intact ultrastructure is very difficult. In this study, 10 intact whole worms (C. hepatica) were isolated from the liver of 3-week-old mouse after inoculation of artificially embryonated eggs collected from house rats (Rattus norvegicus). Their external structure of was observed with light and scanning electron microscopy. The length of the isolated female and male C. hepatica was approximately 69.60 mm and 36.92 mm, respectively. More detailed ultrastructure, including bacillary band, eggs and vulva in female and spicule and spicule sheath in male C. hepatica was also described.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권3호
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• pp.236-238
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• 2004

This is the first report on the antitumor and immunostimulating activities of Ulva lactuca. Using the WSM (water-soluble fraction of a methanol extract from Ulva lactuca), a concentration of 140 g/mL was found to inhibit 50% of the human leukemia cell line U937 in growth, while splenocyte growth was stimulated up to a concentration of 100$\mu\textrm{g}$/mL. In addition, NO production by a macrophage cell line (RAW 264.7) and alkaline phosphatase activity in mouse splenocytes were both stimulated with 10$\mu\textrm{g}$/mL of WSM. Dose-dependent patterns were observed on all three cell-lines. Accordingly, the current results indicate that VUlva lactuca may be useful as a natural antitumor and immunostimulating agent.

• KSBB Journal
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• 제31권1호
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• pp.40-45
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• 2016

Vaginitis, also known as vaginal infection and vulvovaginitis, is an inflammation of the vagina and possibly vulva. The three main kinds of vaginitis are bacterial vaginosis, vaginal candidiasis, and trichomoniasis. The purpose of this study was to characterize Lactobacillus plantarum MG989 and L. fermentum MG901 isolated from the vaginas of healthy Korean women in terms of their inhibitory activity against the vaginitis associated pathogens such as Gardnerella vaginalis and Candida albicans. Co-culture experiments showed that the two Lactobacillus strains MG989 and MG901 significantly reduced the viability of G. vaginalis and C. albicans. Also, the two strains were resistant to bile acid up to 1% and their autoaggregation rates were as high as 83.33%. Further studies are underway to demonstrate that the two strains can be applied as pharmaceutical agents for recovering healthy vaginal ecosystem.

• 한국토양동물학회지
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• 제7권1_2호
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• pp.45-49
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• 2002

골프장에 재식된 들잔디, Zoysia japonica에서 피해 원인을 조사하던 중 뿌리혹선충이 다수 기생하고 있었다. 이 선충의 가해특성과 형태적 특징을 조사하였다. 이 선충은 뿌리에 혹을 형성하지 않으며, 뿌리 겉으로 후반부를 드러낸 암컷은 난낭을 형성하고 있다. M. marylandi의 주요 특징으로는, 암컷의 경우 Perineal 패턴은 둥글며 높게 위치하여 있고, 음문과 항문은 함몰되어 있으며, 물결 모양의 주름은 거칠며 넓게 공간을 형성하고 있다. 유충의 체장은 390.3 $\mu\textrm{m}$ (290-430), 꼬리 길이는 55.1 $\mu\textrm{m}$ (49-59)이며, 4개의 측선을 이룬다 수컷의 구침길이는 18.1 $\mu\textrm{m}$ (15-24), 교접자 길이는 26.2 $\mu\textrm{m}$ (23-29)이며, 4-5개의 측선수를 가진다.

• Animal Systematics, Evolution and Diversity
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• 제36권3호
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• pp.240-250
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• 2020

Member of the genus Tobrilus Andrássy, 1959, which belongs to Tobrilidae Filipjev, 1918, are known as free-living nematodes in freshwater habitat. This genus was previously unknown from Korea. Five Tobrilus species are here reported for the first time from Korea: Tobrilus aberrans (Schneider, 1925), Tobrilus diversipapillatus (Daday, 1905), Tobrilus gracilis (Bastian, 1865), Tobrilus longus (Leidy, 1851), and Tobrilus wesenbergi (Micoletzky, 1925). Specimens were collected from sediments of the Nakdong River in Korea. Morphological characters and measurements of the specimens generally agree with the original descriptions of Tobrilus species, except for some differences that can be attributed to intraspecific variation among populations(e.g., nerve ring position [% pharynx] and reproductive length). Each species can be distinguished from other members of the genus by specific characters (e.g., cephalic setae length and position, buccal cavity and pocket shape, vulva position, degree of development of reproductive system, and tail length and shape). Here, five species in the genus Tobrilus are fully redescribed and illustrated using optical microscopy images. DNA barcode sequence information (the D2-D3 region of 28S rDNA) is also provided for molecular species identification.

• Journal of Microbiology
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• 제42권4호
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• pp.255-266
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• 2004

Papillomaviruses infect a wide variety of animals, including humans. The human papillomavirus (HPV), in particular, is one of the most common causes of sexually transmitted disease. More than 200 types of HPV have been identified by DNA sequence data, and 85 HPV genotypes have been well char­acterized to date. HPV can infect the basal epithelial cells of the skin or inner tissue linings, and are, accordingly, categorized as either cutaneous or mucosal type. HPV is associated with a panoply of clin­ical conditions, ranging from innocuous lesions to cervical cancer. In the early 1980s, studies first reported a link between cervical cancer and genital HPV infection. Genital HPV infections are now rec­ognized to be a major risk factor in at least $95\%$ of cervical cancers. 30 different HPV genotypes have been identified as causative of sexually transmitted diseases, most of which induce lesions in the cervix, vagina, vulva, penis, and anus, as the result of sexual contact. There is also direct evidence demon­strating that at least four of these genotypes are prerequisite factors in cervical cancer. The main aim of this review was to evaluate the current literature regarding the pathovirology, diagnostics, vaccines, therapy, risk groups, and further therapeutic directions for HPV infections. In addition, we reviewed the current status of HPV infections in South Korean women, as evidenced by our data.

• Parasites, Hosts and Diseases
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• 제52권5호
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• pp.531-535
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• 2014

Surveys on helminthic fauna of the nutria, Myocastor coypus, have seldom been performed in the Republic of Korea. In the present study, we describe Strongyloides myopotami (Secernentea: Strongyloididae) recovered from the small intestine of feral nutrias. Total 10 adult nutrias were captured in a wetland area in Gimhae-si (City), Gyeongsangnam-do (Province) in April 2013. They were transported to our laboratory, euthanized with ether, and necropsied. About 1,300 nematode specimens were recovered from 10 nutrias, and some of them were morphologically observed by light and scanning electron microscopies. They were 3.7-4.7 ($4.0{\pm}0.36$) mm in length, 0.03-0.04 (0.033) mm in width. The worm dimension and other morphological characters, including prominent lips of the vulva, blunted conical tail, straight type of the ovary, and 8-chambered stoma, were all consistent with S. myopotami. This nematode fauna is reported for the first time in Korea.

• Asian-Australasian Journal of Animal Sciences
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• 제33권7호
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• pp.1138-1147
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• 2020

Objective: A study was made investigate factors affecting body surface temperature changes after weaning in sows, whether these can be used to aid detection of natural estrus and how they relate to subsequent reproductive performance. Methods: A total of 132 sows were selected during summer from a breeding farm, with mean parity of 3.6±2.3 and 28.5±0.9 days lactation length. Four daily measurements (6:00, 8:00, 16:00, and 18:00) of vulva (VST), udder (UST), ear base and central back skin temperatures for individual sows were taken by an infrared thermometer, continuing up to 8 days post weaning. Results: The VST obtained from sows showing estrus at 4 days post-weaning (4DPW), 5DPW, and 6DPW showed a peak at the fourth day post-weaning, but then started to decrease. The VST of sows not detected in standing heat (NDPW) remained at a lower level during the experiment, but UST was increased soon after weaning. The VST-UST temperature differences during daytime of sows that were showing behavioural standing heat on 4DPW, 5DPW, 6DPW, and 7DPW were 0.46℃±0.123℃, 0.71℃±0.124℃, 0.66℃ ±0.171℃, and 0.58℃±0.223℃, respectively. The NDPW sows had the highest UST observed, but also the lowest VST so that a more negative value of temperature difference (-0.31℃) was seen during first few days post-weaning. A total of 119 sows were observed to show standing heat and were bred. The later the estrus, the smaller the litter size (p = 0.005). Conclusion: Sows which did not show behavior indicative of stable standing heat after weaning had a VST which remained at a lower level, but the UST increased soon after weaning. Therefore, for sow heat detection under field conditions, the changes of VST and UST and difference between the two should be considered together to increase the accuracy of detection.

• Asian Pacific Journal of Cancer Prevention
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• 제17권2호
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• pp.713-717
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• 2016

Background: To evaluate the prevalence and features of other gynecologic or surgical lesions in endometrial cancer (EMC) patients. Materials and Methods: Clinico-pathological data of EMC patients who were treated in the institution from 1995 to 2012 were collected. Data collected were age, stage of disease according to the FIGO 2009 criteria (FIGO), histopathology, tumor grade, adjuvant therapy, other gynecologic or surgical lesions, follow-up period, and living status. Results: The mean age of 396 patients was $56.7{\pm}10.64years$. Abnormal uterine bleeding was the most common presenting symptom (90.1%). Bleeding was accompanied with pelvic mass in 7.7% and 5.4% had only a pelvic mass. Abnormal cervical cytology was found in 3.8%. Approximately 75% had early stage diseases and 86% had endometrioid histology. We found 55.8% of EMC patients had other gynecologic lesions: 89.6% benign and 9.5% malignant. Some 4.5% had pre-invasive cervical/vulva/vagina lesions. The two most common gynecologic lesions were myoma uteri and ovarian tumors. Focusing on the latter, approximately 14% were benign while 8% were malignant. Among 364 patients with available data, surgical lesions were found in 11.8%, 5.7% benign and 9.2% malignant. The most common benign surgical condition was chronic appendicitis while breast and colon cancers were the two most common malignant lesions found. Conclusions: More than half of EMC patients had other gynecologic lesions including benign and malignant tumors. Surgical lesions were also found in more than one-tenth of patients. Careful pre-operative evaluation and intra-operative inspection are advised for proper management and better prognosis.

• Journal of Species Research
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• 제6권2호
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• pp.177-180
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• 2017

Diploscapter coronatus (Cobb, 1893) Cobb, 1913 was first reported on oriental melon (Cucumis melo vars. makuwa Makino) roots from plastic film houses, Gyeongsangbuk-do, Andong, Korea (latitude $N36^{\circ}33^{\prime}$. longitude $E128^{\circ}29^{\prime}$). Diploscapter coronatus is most similar to D. pachys. Species in the genus Diploscapter have a visibly annulated cuticle. The stoma of D. coronatus is 1.5 times longer than the lip region width. The pharyngeal corpus is clearly separated from the isthmus and the vulva is situated around the mid-body. However, it differed by higher ratio of "a", filiform tail and had relatively blunt labial hooks. The Korean population is well matched and within the range of D. coronatus as described from Iran and Ethiopia in de Man's of L, b, c, c', and V. Female length, however, varied between populations: the Ethiopia population female length is longer than in the Korea population (396.4 vs 427.0) while females in the Iran population are smaller than in the Korea population (396.4 vs 350.0). Nematode size may vary due to environmental conditions such as food sources. The position of excretory pores in the Korean population were shorter 53.9-72.5 than in other populations (67-82 and 70-89). Males were uncommon. Males in the Korean population are smaller than females but larger(356.0) than males in the Indian population (306.0).