KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characterization and Inhibitory Activity of Lactobacillus plantarum MG989 and Lactobacillus fermentum MG901 Isolated from Vaginal Microbiota of Korean Women against Gardnerella vaginalis and Candida albicans

한국여성의 질에서 분리한 Lactobacillus plantarum MG989와 Lactobacillus fermentum MG901의 Gardnerella vaginalis와 Candida albicans에 대한 억제효과 및 특성 규명

  • Received : 2016.01.11
  • Accepted : 2016.02.17
  • Published : 2016.03.31
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Abstract

Vaginitis, also known as vaginal infection and vulvovaginitis, is an inflammation of the vagina and possibly vulva. The three main kinds of vaginitis are bacterial vaginosis, vaginal candidiasis, and trichomoniasis. The purpose of this study was to characterize Lactobacillus plantarum MG989 and L. fermentum MG901 isolated from the vaginas of healthy Korean women in terms of their inhibitory activity against the vaginitis associated pathogens such as Gardnerella vaginalis and Candida albicans. Co-culture experiments showed that the two Lactobacillus strains MG989 and MG901 significantly reduced the viability of G. vaginalis and C. albicans. Also, the two strains were resistant to bile acid up to 1% and their autoaggregation rates were as high as 83.33%. Further studies are underway to demonstrate that the two strains can be applied as pharmaceutical agents for recovering healthy vaginal ecosystem.

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References

  1. Egan, M. E. and M. S. Lipsky (2000) Diagnosis of vaginitis. Am. Fam. Physician 62: 1095-1104.
  2. Northrup, C. (2010) Women's bodies, women's wisdom: Creating physical and emotional health and healing. pp. 297-299. Bantam, NY, USA.
  3. Petrova, M. I., E. Lievens, S. Malik, N. Imholz, and S. Lebeer (2015) Lactobacillus species as biomarkers and agents that can promote various aspects of vaginal health. Front. Physiol. 6: 81.
  4. Mastromarino, P., B. Vitali, and L. Mosca (2013) Bacterial vaginosis: a review on clinical trials with probiotics. New Microbiol. 36: 229-238.
  5. Chen, K. C., P. S. Forsyth, T. M. Buchanan, and K. K. Holmes (1979) Amine content of vaginal fluid from untreated and treated patients with nonspecific vaginitis. J. Clin. Invest. 63: 826-835.
  6. Verstraelena, H. and A. C. Senok (2005) Vaginal lactobacilli, probiotics, and IVF. Reprod. Biomed. Online 11: 674-675. https://doi.org/10.1016/S1472-6483(10)61683-5
  7. Wiesenfeld, H. C., S. L. Hillier, M. A. Krohn, D. V. Landers, and R. L. Sweet (2003) Bacterial vaginosis is a strong predictor of Neisseria gonorrhoeae and Chlamydia trachomatis infection. Clin. Infect. Dis. 36: 663-668. https://doi.org/10.1086/367658
  8. Berman, J. and P. E. Sudbery (2002) Candida albicans: A molecular revolution built on lessons from budding yeast. Nat. Rev. Genet. 3: 918-930.
  9. Wenzel, R. P. (1995) Nosocomial candidiasis: Risk factors and attributable mortality. Clin. Infect. Dis. 20: 1531-1534. https://doi.org/10.1093/clinids/20.6.1531
  10. Bradshaw, C. S., A. N. Morton, J. Hocking, S. M. Garland, M. B. Morris, L. M. Moss, L. B. Horvath, I. Kuzevska, and C. K. Fairley (2006) High recurrence rates of bacterial vaginosis over the course of 12 months after oral metronidazole therapy and factors associated with recurrence. J. Infect. Dis. 193: 1478-1486. https://doi.org/10.1086/503780
  11. Redondo-Lopez, V., R. L. Cook, and J. D. Sobel (1990) Emerging role of lactobacilli in the control and maintenance of the vaginal microflora. Rev. Infect. Dis. 12: 856-872. https://doi.org/10.1093/clinids/12.5.856
  12. Sharpe, M. E. (1981) The genus Lactobacillus. pp. 1653-1679. In: M. P. Starr, H. Stolp, H. G. Truper, A. Balows, and L. Schlegel (eds.). The prokaryote: a handbook on habitats, isolation and identification of bacteria. Springer-Verlag, NY, USA.
  13. Vallor, A. C., A. D. A. May, E. H. Stephen, and L. H. Sharon (2001) Factors associated with acquisition of, or persistent colonization by, vaginal lactobacilli: role of hydrogen peroxide production. J. Infect. Dis. 184: 1431-1436. https://doi.org/10.1086/324445
  14. Bastani, P., A. Homayouni, S. Ziyadi, and V. G. Tabrizian (2012) Dairy probiotic foods and bacterial vaginosis: A review on mechanism of action. pp. 445-456. In: E.C. Rigobelo (ed.). Probiotics. Intech, USA.
  15. Guo, X. H., J. M. Kim, H. M. Nam, S. Y. Park, and J. M. Kim (2010) Screening lactic acid bacteria from swine origins for multistrain probiotics based on in vitro functional properties. Anaerobe 16: 321-326. https://doi.org/10.1016/j.anaerobe.2010.03.006
  16. Kassa, I. A., M. Hamze, D. Hober, N. E. Chihib, and D. Drider (2014) Identification of vaginal lactobacilli with potential probiotic propertied isolated from women in north lebanon. Microb. Ecol. 67: 722-734. https://doi.org/10.1007/s00248-014-0384-7
  17. Pavlova, S. I., A. O. Kilic, S. S. Kilic, J. S. So, M. E. Nader-Macias, J. A. Simoes, and L. Tao (2002) Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences. J. Appl. Microbiol. 92: 451-459. https://doi.org/10.1046/j.1365-2672.2002.01547.x
  18. Borchardt, K. A., B. S. Adly, R. F. Smith, J. Eapen, and C. B. Beal (1989) Importance of Gardnerella vaginalis as an aetiological agent in bacterial vaginosis. Genitourin. Med. 65: 285.
  19. Borge, S., J. Silva, and P. Teixeira (2013) The role of lactobacilli and probiotics in maintaining vaginal health. Arch. Gynecol. Obstet. 289: 479-489.
  20. Sobel, J. D. (2007) Vulvovaginal candidosis. The Lancet 369: 1961-1971. https://doi.org/10.1016/S0140-6736(07)60917-9
  21. Samaranayake, L. P., A. Hughes, D. A. Weetman, and T. W. Mac-Farlane (1986) Growth and acid production of Candida species in human saliva supplemented with glucose. J. Oral Pathol. 15: 251-254. https://doi.org/10.1111/j.1600-0714.1986.tb00617.x
  22. Köhler, G. A., S. Assefa, and G. Reid (2012) Probiotic Interference of Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 with the opportunistic fungal pathogen Candida albicans. Infect. Dis. Obstet. Gynecol. 2012: 14.
  23. Dunne, C., L. O’Mahony, L. Murphy, G. Thornton, D. Morrissey, S. O’Halloran, M. Feeney, S. Flynn, G. Fitzgerald, C. Daly, B. Kiely, G. C. O’Sullivan, F. Shanahan, and J. K. Collins (2001) In vitro selection criteria for probiotic bacteria of human origin: Correlation with in vivo findings. Am. J. Clin. Nutr. 73: 386-392. https://doi.org/10.1093/ajcn/73.2.386s
  24. Gilliland, S. E., T. E. Staley, and L. J. Bush (1984) Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct. J. Dairy Sci. 67: 3045-5301. https://doi.org/10.3168/jds.S0022-0302(84)81670-7
  25. Lin, W. H., C. F. Hwangb, L. W. Chenc, and H. Y. Tsen (2006) Viable counts, characteristic evaluation for commercial lactic acid bacteria products. Food Microbiol. 23: 74-81. https://doi.org/10.1016/j.fm.2005.01.013
  26. Osset, J., R. M. Bartolomé, E. García, and A. Andreu (2001) Assessment of the capacity of Lactobacillus to inhibit the growth of uropathogens and block their adhesion to vaginal epithelial cells. J. Infect. Dis. 183: 485-491. https://doi.org/10.1086/318070
  27. Malik, S., M. I. Petrova, I. J. J. Claes, T. L. A. Verhoeven, P. Busschaert, M. Vaneechoutte, B. Lievens, I. Lambrichts, R. J. Siezen, J. Balzarini, J. Vanderleyden, and S. Lebeer (2013) The highly autoaggregative and adhesive phenotype of the vaginal Lactobacillus plantarum strain CMPG5300 is sortase dependent. Appl. Environ. Microbiol. 79: 4576-4585. https://doi.org/10.1128/AEM.00926-13

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