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http://dx.doi.org/10.9729/AM.2013.43.4.146

Ultrastructure of Capillaria hepatica (Syn. Calodium hepatica) Isolated from the Liver of Mouse Infected with Artificially Embryonated Eggs Collected from House Rats (Rattus norvegicus)  

Min, Byoung-Hoon (Department of Parasitology and the Institute of Travel Medicine, Korea University College of Medicine)
Lee, Haeng-Sook (Department of Parasitology and the Institute of Travel Medicine, Korea University College of Medicine)
Kim, Soo-Jin (Department of Life Science, College of Natural Science, Hallym University)
Joo, Kyoung-Hwan (Department of Parasitology and the Institute of Travel Medicine, Korea University College of Medicine)
Publication Information
Applied Microscopy / v.43, no.4, 2013 , pp. 146-154 More about this Journal
Abstract
Capillaria hepatica (syn. Calodium hepatica) is a parasite found mainly in rodent liver. But, it has also been found in a wide variety of mammals, including humans. This worm is unique as it is the only nematode parasite that is embedded in the liver parenchyma of the host even during the adult stage of the life cycle. They produce eggs that elicit a marked granulomatous reaction that eventually destroys the worms. Fibrosis and lymphoplasmacytic inflammatory infiltration are often observed around adult nematodes embedded in the liver parenchyma of the host. For this reason, complete isolation of this slender worm and observation of the intact ultrastructure is very difficult. In this study, 10 intact whole worms (C. hepatica) were isolated from the liver of 3-week-old mouse after inoculation of artificially embryonated eggs collected from house rats (Rattus norvegicus). Their external structure of was observed with light and scanning electron microscopy. The length of the isolated female and male C. hepatica was approximately 69.60 mm and 36.92 mm, respectively. More detailed ultrastructure, including bacillary band, eggs and vulva in female and spicule and spicule sheath in male C. hepatica was also described.
Keywords
Capillaria hepatica; Genital organ; Spicule; Scanning electron microscopy;
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