• 한국작물학회:학술대회논문집
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• 한국작물학회 2019년도 추계학술대회
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• pp.22-22
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• 2019

Green stem disorder (GSD) of soybean (Glycine max (L.) Merr.) is characterized by delayed senescence of stems with normal pod ripening and seed maturation (Hobbs, 2006). GSD complicates harvesting of soybeans by significantly increasing the difficulty in cutting the affected plants. There is also the potential for moisture in the stems to be scattered on the seed, reducing the grade and storability of the seed. Not only the cause of GSD is yet unknown, but also GSD cannot be evaluated until maturity, therefore the method to evaluate GSD in early growth stage with high sensitivity is necessary. In previous studies, it has been reported that vegetative storage protein (VSP) accumulates and the syndrome of GSD appears in soybean after depod treatment (Fischer, 1999). Soybean VSP is a storage protein which is abundant in young sink leaves and degraded during seed fill (Wittenbach, 1982). Hence, we have established a system to quantify VSP of high sensitivity by using standard protein made by genetically transformed E. coli and specific antibody against VSP, and studied the relationship between VSP and GSD, by depod experiment and drought/excess wet experiments. The result of depod experiment with the cultivar 'Yukihomare' was the same with the previous studies, VSP accumulated much more than control and the syndrome of GSD appeared in soybean in depod treatment. Drought and excess wet had different impact on GSD. Excess wet caused GSD of the cultivar 'Tachinagaha (GSD susceptible)', while drought caused a little syndrome of GSD in the cultivar 'Touhoku 129 (GSD resistant)'. The accumulation of VSP differed between the two cultivars over time. In conclusion, the accumulation of VSP came along with the emergence of GSD. Different cultivars showed different response to drought and excess wet. In the future, it is expected that the dynamics of VSP will be elucidated in detail, leading to the development of early diagnosis technology for green stem disorder and the elucidation of mechanism of soybean GSD.

• Asian-Australasian Journal of Animal Sciences
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• 제27권9호
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• pp.1336-1344
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• 2014

The effects of various packaging systems, vacuum packaging (VACP), medium oxygen-modified atmosphere packaging (50% $O_2/20%$ $CO_2/30%$ $N_2$, MOMAP), MOMAP combined with vacuum skin packaging (VSP-MOMAP), high oxygen-MAP (80% $O_2/20%$ $CO_2/30%$ $N_2$, HOMAP), and HOMAP combined with VSP (VSP-HOMAP), on the activity of antioxidant enzyme, and oxidation and color stabilities in sliced Hanwoo (Korean cattle) beef loin were investigated at $4^{\circ}C$ for 14 d. Higher (p<0.05) superoxide dismutase activity and total reducing ability were maintained in VSP-MOMAP beef than in HOMAP beef. Lipid oxidation (2-thiobarbituric acid reactive substances, TBARS) was significantly (p<0.05) retarded in MOMAP, VSP-MOMAP, and VSP-HOMAP beef compared with HOMAP beef. Production of nonheme iron content was lower (p<0.05) in VSP-MOMAP beef than in HOMAP beef. Red color ($a^*$) was kept higher (p<0.05) in VSP-MOMAP beef compared with MOMAP, HOMAP, and VSP-HOMAP beef. However, VACP beef was found to have the most positive effects on the antioxidant activity, oxidation and red color stabilities among the various packaged beef. These findings suggested that VSP-MOMAP was second to VACP in improving oxidation and color stabilities in sliced beef loin during chill storage.

• 지구물리와물리탐사
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• 제12권3호
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• pp.255-262
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• 2009

가스하이드레이트 개발 시 중요한 요소 중의 하나인 가스하이드레이트 부존층의 정확한 심도를 얻기 위해서는 일반적으로 고해상도 정보를 주는 VSP 탐사가 많이 수행되어왔다. 본 연구에서는 가스하이드레이트 부존지역인 울릉분지 지역의 UBGH09 시추공에서 국내 최초로 삼성분 지오폰을 사용하여 획득한 제로오프셋 VSP 탐사자료로부터 구간속도를 추출하고 corridor 중합단면도를 생성하여 지표탄성파탐사 자료와 비교해 보았다. 먼저 Phase rotation을 통하여 삼성분 지오폰으로 기록된 신호들이 실제 수직 및 두 개의 수평성분 자료들이 되도록 변환 시키고, 파동장 분리과정을 통하여 직접파와 반사파를 분리하였다. 제로오프셋 VSP 자료의 직접파로부터 추출한 구간속도를 음파검층의 구간속도와 비교한 결과 두 구간속도 모두 비슷한 양상을 나타냈다. BSR 상부의 하이드레이트 층에서는 빠른 속도를 보이고 BSR 하부에서 속도가 급격히 떨어지는 것으로 보아 BSR 하부에 유리가스가 집적되어 있는 것으로 보인다. 제로오프셋 VSP 반사파 자료로부터 corridor 중합단면도를 생성하여 지표탄성파탐사의 CMP 중합단면도와 비교한 결과 대부분의 반사이벤트들이 잘 부합하였으며, phase rotation을 적용한 결과가 phase rotation을 적용하지 않았을 경우보다 더 잘 부합하였다.또한 transverse 성분을 이용하여 만든 corridor 중합단면도와 지표탄성파 CMP 중합단면도를 비교한 결과 지표탄성파 CMP 중합 단면도 내의 모오드 변환된 PS 반사파들을 확인할 수 있었다.

• 지구물리와물리탐사
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• 제14권3호
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• pp.227-233
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• 2011

지열은 외부 연료 공급요건에 영향을 받지 않고 연중 가동할 수 있는 장점을 가지기 때문에 최근 해외에서 주목받고 있는 대체 에너지원이다. 그리고 국내의 온천 및 지열이상대는 화강암 지역의 심부 파쇄대를 통한 지열수의 순환에 의한 것이 대부분으로 알려져 있다. 따라서 국내에서의 지열탐사는 지하유체의 주요 통로인 심부 파쇄대의 분포상황을 정확하게 파악하는 것이 매우 중요하다. 본 연구에서는 석모도에서 취득한 수직 탄성파탐사(Vertical Seismic Profiling, VSP) 자료의 전처리와 심부 파쇄대의 영상화를 수행하기 위하여 급경사 파쇄대 지역과 퇴적층 구조를 모사한 속도모델로부터 합성탄성파 자료를 생성하고, 이 자료에 일반적인 VSP 자료처리기법과 급경사 파쇄대를 고려한 VSP 자료처리기법을 각각 적용한 결과를 비교 분석하였다. 그 결과 파쇄대에 의한 반사 이벤트의 손실을 없애기 위해서는 단순히 모든 하향파를 제거하는 일반적인 전처리 과정이 아닌 파쇄대를 고려한 전처리 과정이 필요하다는 것을 확인하였다. 또한 파쇄대를 고려한 전처리 과정이 수행된 석모도 거꿀 수직 탄성파탐사(Reversed VSP, RVSP) 현장자료에 3차원 겹쌓기전 위상막 참 반사보정을 적용하여 석모도 탐사현장의 지하구조를 영상화하였다. 참 반사보정 결과 파쇄대로 추정되는 구조가 확인되었으며, 이는 시추공 자료에서 추정되는 균열대의 심도와 일치하였다.

• 생명과학회지
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• 제21권7호
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• pp.1032-1038
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• 2011

최근에 Ciona intestinalis에서 확인된 막 전위 감지 탈인산화 효소는 이온 채널과 유사한 막 통과 도메인과 유사 탈인산화 효소 도메인으로 이루어져 있다. 이 Ci-VSP는 그 막 통과 도메인에 의해 막 전위 변화를 감지하고 유사 탈인산화 효소에 의해 인산화된 이노시틸 인지질들에 대한 탈인산화 활성을 보이는 것으로 알려져 있다. 본 연구는 6개의 히스티딘이 융합된 Ci-VSP의 유사 탈인산화 효소 도메인의 발현 시스템 구축을 추구하였다. 그래서 본 논문은 그 시스템의 구축과 발현 그리고 정제 조건 확립, 마지막으로 그 효소 동력학적인 활성을 pNPP에 대한 검토한 결과를 보고한다. 본 연구 결과에 따르면 Ci-VSP(248-576)-His 단백질 발현 및 정제시에 다른 단백질들의 정제 조건과 다르게 25 mM NaCl과 100 mM 정도의 이미다졸이 필요함을 확인하였다. 정제된 단백질을 가지고 탈인산화 효소의 대표적인 기질인 pNPP를 이용하여 그 동력학적인 상수 측정을 시도한 결과 정상 상태 동력학 측정 조건으로 온도 $37^{\circ}C$, pH 5.0 내지 5.5, 반응 시간 30분 이내, 반응 단백질 양 $2.0\;{\mu}g$ 이내가 적합하다는 점을 알게 되었다. 이러한 확립된 조건을 토대로 pNPP에 대한 동력학적 상수를 측정한 결과 $K_m$ 값은 $354{\pm}0.143\;{\mu}M$이며 $V_{max}$는 $0.0607{\pm}0.0137\;{\mu}mol$/min/mg이며 $k_{cat}$ 값은 $2.359{\pm}0.009751\;min^{-1}$인 것으로 확인되었다. 이를 통해 본 연구는 Ci-VSP(248-576)-His의 순도 높은 정제 결과와 pNPP에 대한 높은 활성 보임을 제시하였다. 이러한 연구 결과를 통해 향후 Ci-VSP에 대한 구조적인 연구 등을 포함하는 다양한 생화학적인 연구가 수행되리라 본다.

• 폴리머
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• 제24권6호
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• pp.757-762
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• 2000

4-히드록시스티릴피리딘과 4-비닐벤질클로리드를 반응시켜 감광성 단량체로서 비닐벤질옥 시스티릴피리딘 (VSP)을 합성하였다. 5-20 mo1%의 VSP를 로진 moiety를 함유하는 메타크릴단량체와 라디칼 공중합하여 로진 moiety를 함유하는 몇가지 광경화성 고분자를 합성하였다. UV분광법으로 결정한 공중합체 중의 VSP의 함량은 5.3-17.3mol%정도였다. 이들 고분자의 수평균 분자량은 18000-28000정도였고 다분산지수는 1.8 정도였으며, 또한 유리전이온도는 15$0^{\circ}C$ 부근이였고 초기 열분해온도는 34$0^{\circ}C$ 정도였다. 이들 고분자는 자외선에 의해 비교적 빠르게 경화되었고, 이 광경화 반응은 UV분광법과 잔막수율법으로 분석될 수 있었다.

• 지구물리와물리탐사
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• 제7권1호
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• pp.70-79
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• 2004

We have examined the applicability of the triaxial drill-bit VSP method (TAD-VSP) to the geological survey of possible sites for a high-level radioactive waste disposal repository. The seismic energy generated by a drill bit is measured by a downhole multi-component detector, and the resulting signals are processed to image the geological structure deep underground. In order to apply the TAD-VSP method to civil-engineering-scale drilling, we have developed a small but highly sensitive and precise three-component downhole seismic measurement system, and recorded drill-bit signals at a granite quarry. We have successfully imaged discontinuities in the granite, possibly related to fractures, as highly reflective zones. The discontinuities imaged by the TAD-VSP method correlate well with the results of other borehole observations. In conclusion, the TAD-VSP method is usable in geological investigations for civil engineering because the equipment is compact and it is simple to acquire the drill-bit signal.

• 한국유기농업학회지
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• 제27권3호
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• pp.353-363
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• 2019

To identify four cyst nematodes (Heterodera schachtii, H. trifolii, H. glycines, H. sojae) that are economically important plant-parasitic nematodes in Korea, restriction fragment length polymorphism (RFLP) by 8 endonucleases (PstI, VspI, AlwI, RsaI, MvaI, EcoRI, Eco72I, Hinf I) was performed based on sequence difference of mitochondrial DNA cytochrome c oxidase subunit I (COI) gene. As a result, species-specific DNA band patterns by RsaI endonuclease were observed in H. schachtii. The specific patterns was in H. trifolii by 3 endonucleases (VspI, AlwI, Hinf I), and was in H. glycines by Hinf I. While, H. sojae was not digested by 4 endonuclease (VspI, AlwI, RsaI, Hinf I). This study showed that four cyst nematodes could be distinguished using RFLP by 4 endonucleases (RsaI, VspI, AlwI, Hinf I) based on the sequence difference of COI gene.

• 한국축산식품학회지
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• 제34권3호
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• pp.273-279
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• 2014

The objective of this study was to investigate the effects of the packaging method on the lipid and protein oxidation, and color in aged top round from Hanwoo (Korean native cattle) for 14 d at $4^{\circ}C$. Catalase activity was the highest (p<0.05) in vacuum packaging (VP) treatment during storage, and was higher (p<0.05) in 50% Ox-MAP and 50% Ox-MAP+vacuum skin packaging (VSP) treatments than in other treatments at d 14. Superoxide dismutase activity was higher (p<0.05) in VP, 50% Ox-MAP, and 50% Ox-MAP+VSP treatments than in other treatments at d 14. During storage, total antioxidant activity was the highest (p<0.05) in VP treatment and was higher (p<0.05) in 50% Ox-MAP+VSP treatment than in 80% Ox-MAP treatment. TBARS value was the lowest (p<0.05) in VP treatment during storage and was lower (p<0.05) in 50% Ox-MAP and Ox-MAP+VSP treatments than in 80% Ox-MAP and Ox-MAP treatments, respectively. Carbonyl content was the lowest (p<0.05) in VP treatment from 10 d. From 7 d, the $a^*$ value was the highest (p<0.05) in VP treatment and was higher (p<0.05) in 50% Ox-MAP and 50% Ox-MAP+VSP treatments than in other treatments. The $b^*$ value was the highest (p<0.05) in VP treatment from 3 d, and was higher (p<0.05) in 80% Ox-MAP+VSP, 50% Ox-MAP, and 50% Ox-MAP+ VSP treatments than in 80% Ox-MAP treatment at d 14. Therefore, VP improved the oxidation and red color stabilities in stored-aged top round compared with Ox-MAP. In addition, 50% Ox-MAP improved the lipid oxidation and red color stabilities compared with 80% Ox-MAP, and its inhibitory effect on lipid oxidation was enhanced by combination with VSP.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제41권
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• pp.35.1-35.7
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• 2019

Background: The purpose of this study was to measure the time of the conventional surgical planning (CSP) and virtual surgical planning (VSP) in orthognathic surgery and to compare them in terms of cost. Material and method: This is a retrospective study of the patients who underwent orthognathic surgery at the Pusan National University Dental Hospital from December 2017 to August 2018. All the patients were analyzed through both CSP and VSP, and all the surgical stents were fabricated through manual and three-dimensional (3D) printing. The predictor variables were the planning method (CSP vs. VSP) and the surgery type (group I: Le Fort I osteotomy + bilateral sagittal split osteotomy [LFI+BSSO] or group II: only bilateral sagittal split osteotomy [BSSO]), and the outcomes were the time and cost. The results were analyzed using the paired t test. Results: Thirty patients (12 females, 18 males) met the inclusion criteria, and 17 patients were excluded from the study due to missing or incomplete data. There were 20 group I patients (LFI+BSSO regardless of genioplasty) and 10 group II patients (BSSO regardless of genioplasty). The average time of CSP for group I was 385 ± 7.8 min, and that for group II was 195 ± 8.33 min. The time reduction rate of VSP compared with CSP was 62.8% in group I and 41.5% in group II. On the other hand, there was no statistically significant cost reduction. Conclusions: The time investment in VSP in this study was significantly smaller than that in CSP, and the difference was greater in group I than in group II.