• 생명과학회지
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• 제14권2호
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• pp.362-367
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• 2004

장염비브리오균의 숙주 내 감염을 일으키는 기작을 이해하기 위하여 세포외 효소 중의 하나인 콜라겐분해효소의 유전자가 불활성화된 돌연변이체를 제작하였다. 콜라겐분해효소의 유전자인 vppC 유전자에 항생제 내성 유전자인 nptII를 삽입하여 제작된 재조합 DNA를 suicide vector인 pDMS197에 클로닝하여 pVCM03이라 명명하였다. 재조합 suicide 플라스미드 pVCM03을 E. coli 7213에 형질전환하여 접합을 통하여 원 균주인 V. varahaemolyticus 04에 전달하였다. 전달된 pVCM03 유래의 재조합 vvpC::npfII DNA는 homologous recombination에 의해 wild-type allele와 교환되어 돌연변이체를 형성하게 되고, 돌연변이체는 10% sucrose가 함유된 TCBS 배지에서 선별되었다. Allele exchange는 PCR에 의한 증폭된 DNA의 크기 비교로 확인하였다. 돌연변이체인 V. parahaemolyticus CM은 원 균주와 비교하였을 때 약 4배정도 낮은 콜라겐 분해 활성을 나타내었고, vero cell을 이용한 MTT assay에서도 원 균주에 비하여 낮은 세포독성을 보였다.

• 한국정보전자통신기술학회논문지
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• 제12권3호
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• pp.312-320
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• 2019

본 논문에서는 110nm eFlash 셀을 사용한 512Kb eFlash IP를 설계하였다. eFlash 셀의 프로그램, 지우기와 읽기 동작을 만족시키는 row 구동회로(CG/SL 구동회로), write BL 구동회로( write BL 스위치 회로와 PBL 스위치 선택 회로), read BL 스위치 회로와 read BL S/A 회로와 같은 eFlash 코어회로(Core circuit)를 제안하였다. 그리고 프로그램 모드에서 9.5V와 erase 모드에서 11.5V의 VPP(Boosted Voltage) 전압을 공급하는 VPP 전압 발생기회로는 기존의 단위 전하펌프 회로로 cross-coupled NMOS 트랜지스터를 사용하는 대신 body 전압을 ground에 연결된 12V NMOS 소자인 NMOS 프리차징 트랜지스터의 게이트 노드 전압을 부스팅하는 회로를 새롭게 제안하여 VPP 단위 전하펌프의 프리차징 노드를 정상적으로 VIN(Input Voltage) 전압으로 프리차징 시켜서 VPP 전하펌프 회로의 펌핑 전류를 증가시켰다. 펌핑 커패시터로는 PMOS 펌핑 커패시터에 비해 펌핑전류가 크고 레이아웃 면적이 작은 12V native NMOS 펌핑 커패시터를 사용하였다. 한편 110nm eFlash 공정을 기반으로 설계된 512Kb eFlash 메모리 IP의 레이아웃 면적은 $933.22{\mu}m{\times}925{\mu}m(=0.8632mm^2)$이다.

• 한국정보전자통신기술학회논문지
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• 제13권1호
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• pp.48-57
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• 2020

Wireless charger, USB type-C 등의 응용에서 사용되는 MCU는 추가 공정 마스크가 작으면서 셀 사이즈가 작은 MTP 메모리가 요구된다. 기존의 double poly EEPROM 셀은 사이즈가 작지만 3~5 장 정도의 추가 공정 마스크가 요구되고, FN 터널링 방식의 single poly EEPROM 셀은 셀 사이즈가 큰 단점이 있다. 본 논문에서는 vertical PIP 커패시터를 사용한 110nm MTP 셀을 제안하였다. 제안된 MTP 셀의 erase 동작은 FG와 EG 사이의 FN 터널링을 이용하였고 프로그램 동작은 CHEI 주입 방식을 사용하므로 MTP 셀 어레이의 PW을 공유하여 MTP 셀 사이즈를 1.09㎛2으로 줄였다. 한편 USB type-C 등의 응용에서 요구되는 MTP 메모리 IP는 2.5V ~ 5.5V의 넓은 전압 범위에서 동작하는 것이 필요하다. 그런데 VPP 전하펌프의 펌핑 전류는 VCC 전압이 최소인 2.5V일 때 가장 낮은 반면, 리플전압은 VCC 전압이 5.5V일 때 크게 나타난다. 그래서 본 논문에서는 VCC detector 회로를 사용하여 ON되는 전하펌프의 개수를 제어하여 VCC가 높아지더라도 펌핑 전류를 최대 474.6㎂로 억제하므로 SPICE 모의실험을 통해 VPP 리플 전압을 0.19V 이내로 줄였다.

• 대한미생물학회지
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• 제34권5호
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• pp.423-433
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• 1999

A novel bacteriophage, designated as VPP97, that infects the strains of Vibiro parahaemolyticus (hallophilic, Gram-negative bacterium) isolated most commonly from marine environments, has been discovered, and several of its properties have been determined. The plaques were clear and sized $0.6{\sim}1.0\;mm$ in diameter. The virion forms a single band on 70% sucrose gradient and ${\rho}1.50$ CsCl gradient by sucrose gradient centrifugation and CsCl gradient centrifugation respectively. It has a hexagonal head and a relatively long tail, as shown by electron microscopy. Vibrio alginolyticus, Vibrio fluvialis and Vibrio furnissii were also sensitive to this phage. It was almost totally inactivated at $70^{\circ}C$ and at pH below 5 or over 10. The nucleic acid of VPP97 is composed of DNA. The VPP97 had 9 specific structural proteins sized between 21.5 kDa and 97.4 kDa on SDS-PAGE. When V. parahaemolyticus cultures were treated with either phage VPP97 or one of the several antibiotics for 2 hours, the viable number of V. parahaemolyticus treated with the phage VPP97 is lower than that treated with chloramphenicol, erythromycin or penicillin, but not lower than that treated with tetracycline. Mice that have responded to the phage treatment revealed the lower numbers of V. parahaemolyticus in small intestine and less damage on small intestine compared to the untreated mice. Therefore, we suggest that the phage treatment appears effective to the infection by V. parahaemolyticus.

• 전자공학회논문지C
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• 제34C권4호
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• pp.16-25
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• 1997

This paper shows a SDRAM opeating in 200MHz clock cycle which it use data interleave and pipelining for high speed operation. We proposed NdC (Negative DEaly circuit) to improve clock to access time(tAC) characteristics, also we proposed low power WL(wordline)driver circit and high efficiency VPP charge-pump circit. Our all circuits has been fabricated using 0.4um CMOS process, and the measured maximum speed is 200Mbytes/s in LvTTL interface.

• 전기학회논문지P
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• 제63권1호
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• pp.40-45
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• 2014

This study examines the system and process of battery stored energy in vehicles and suggest the effective area for the use of V2G(vehicle-to-grid) from Jeju Smart Grid Demonstration Project. V2G means technology of electric power transmission from the battery of electric-drive vehicles to state grid. As for the increasing of effectiveness for demand-side control, V2G is a very good alternative. In the U.S., the utilization of electric vehicles is under 40% on average. In this case, we can use he battery of electric vehicle as role of frequency regulation or generator of demand-side resource. V2G, which is the element of Smart Transportation, consists of electric vehicle battery, BMS(battery management system), OBC(on-board charger), charging infrastructure, NOC(network operating center) and TOC(total operation center). V2G application has been tested for frequency regulation to secure the economical efficiency in the United States. In this case, the battery cycle life is not verified its disadvantage. On the other hand, Demand Response is required by low c-rate of battery in electric vehicle and It can be small impact on the battery cycle life. This paper concludes business area of demand response is more useful than frequency regulation in V2G application of electric vehicles in Korea. This provides the opportunity to create a new business for power grid administrator with VPP(virtual power plant).

• Tuberculosis and Respiratory Diseases
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• 제43권6호
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• pp.903-915
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• 1996

Background: Combination chemotherapy is now considered to be the cornerstone of small cell lung cancer (SCLC). management but the optimal management of limited SCLC is not well defined. The role of thoracic radiotherapy (TRT) is less well established. Recent meta-analyses reports revealed that TRT combined with chemotherapy produce "good" local control and prolonged survival. But other reports that survival was not changed. The liming, dose, volume and fractionation for TRT with the combined chemotherapy of SCLC remains unsettled. In this study, we analyzed the effects according to the timing of thoracic radiotherapy in limited SCLC. Method: All fifty one patients received cytoxan, adriamycin and vincristine(CAV) alternating with etoposide and cisplatin(VPP) every 3 weeks for 6 cycles were randomized prospectively into two groups: concurrent and sequential. 27 patients received 4500cGy in 30 fractions(twice daily 150cGy fractional dose) over 3 weeks 10 the primary site concurrent with the first cycle of VPP(concurrent gorup). 24 patients received 4000 to 5000cGy over 5 or 6 weeks after completion of sixth cycles of chemotherapy(sequential group). Results: 1. Response rates and response duration : Response rates were not significantly different between two groups(p=0.13). But response duration was superior in the concurrent group(p=0.03). 2. Survival duration was nor different between two groups(p=0.33). 3. Local control rate was superior in the concurrent group(p=0.00). 4. Side effects and toxicities: Hematologic toxicities, especially leukopenia, infection and frequency of radiation esophagitis were higher in the concurrent group (p=0.00, 0.03, 0.03). Conclusion: The concurrent use of TRT with chemotherapy failed to improve the survival of limited stage SCLC patients compared with the sequential use of TRT but response duration and local control rate were superior in the concurrent group. Frequency of radiation esophagitis, life threatening hematologic toxicities and infection were more frequent in the concurrent group than sequential group. So, the selection of an optimal schedule of chemotherapy combined with TRT that would lead to a major increase in survival with minimal toxicity is remained to be validated in large scale study in the future.

• 센서학회지
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• 제28권4호
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• pp.205-215
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• 2019

A flexible polyvinylidene fluoride (PVDF)/polydimethylsiloxane (PDMS) composite prototype with high piezoelectricity and force sensitivity was constructed, and its huge potential for applications such as biomechanical energy harvesting, self-powered health monitoring system, and pressure sensors was proved. The crystallization, piezoelectric, and electrical properties of the composites were characterized using an X-ray diffraction (XRD) experiment and customized experimental setups. The composite can sustain up to 100% strain, which is a huge improvement over monolithic PVDF fibers and other PVDF-based composites in the literature. The Young's modulus is 1.64 MPa, which is closely matched with the flexibility of the human skin, and shows the possibility for integrating PVDF/PDMS composites into wearable devices and implantable medical devices. The $300{\mu}m$ thick composite has a 14% volume fraction of PVDF fibers and produces high piezoelectricity with piezoelectric charge constants $d_{31}=19pC/N$ and $d_{33}=34pC/N$, and piezoelectric voltage constants $g_{31}=33.9mV/N$ and $g_{33}=61.2mV/N$. Under a 10 Hz actuation, the output voltage was measured at 190 mVpp, which is the largest output signal generated from a PVDF fiber-based prototype.

• 전자공학회논문지
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• 제51권10호
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• pp.57-63
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• 2014

본 논문은 전력소모와 면적을 줄인 지연된 피드-포워드 경로를 갖는 3차 SDM 구조를 제안하였다. 제안한 SDM은 기존의 적분기 2개로 구현된 3차 SDM(Sigma-Delta Modulator) 구조를 개선하였다. 제안된 구조에서는 기존 구조의 둘째 단에 지연된 피드-포워드 경로를 삽입함으로써 첫째 단의 계수 값을 2배로 증가시킬 수 있어 기존구조에 비하여 첫째 단 적분기 커패시터($C_I$)를 1/2로 감소시킬 수 있다. 그러므로 첫째 단 적분기의 부하 커패시턴스가 1/2로 작아지기 때문에 첫째 단 연산증폭기의 출력전류는 51%, 첫째 단의 커패시터 면적은 48% 감소되어 제안한 구조는 전력과 면적을 최적화 할 수 있다. 본 논문에서 제안한 구조를 이용하여 설계된 3차 SC SDM은 $0.18{\mu}m$ CMOS 공정에서 공급전압 1.8V, 입력신호 1Vpp/1KHz, 신호대역폭 24KHz, 샘플링 주파수 2.8224MHz 조건으로 시뮬레이션 하였다. 그 결과 SNR(Signal to Noise Ratio) 88.9dB, ENOB(Effective Number of Bits) 14비트이고 SDM의 전체 전력소모는 $180{\mu}W$이다.

• 대한조선학회논문집
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• 제43권4호
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• pp.504-511
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• 2006

In this paper a modern sailing boat for our costal area was developed by using the traditional boat hull form. Main particulars of design ship were determined by using the statistical values. Hull form was varied from the traditional boat hull form which was selected by the previous study and modified to fit Lateen sail. The appendage, rudder and keel were designed for good performance of against wind sailing. The performance of hull form was examined and the results were shown.