• 대한바이러스학회지
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• 제26권1호
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• pp.67-76
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• 1996

The methods that make Hantavirus grow consist of inoculation into the experimental animals and cultured cells. The cultured cells, such as Vero-E6 and A549 cells, have been usually used for isolation of the virus and the animals, such as mice and rats, are used for large scale preparation of the virus so far. Furthermore, the cell can be used to maintain the virus and assay the infectivity and the animals can be used for the experiment of viral pathogenicity and challenge for assessment of vaccine. Apodemus mice, the own natural host of the virus, has been used for challenge test of Hantaan virus. However it has been pointed out to difficult handling and breeding the animal in laboratory. Therefore, we attempted to establish a new animal model for challenge test at the time of isolation of Maaji virus which is a new hantavirus similar but distinct to Hantaan virus. In suckling hamster, the titer of Maaji virus and the lethality to mice of the virus were increased gradually in the titer and lethality through passage by intracerebral (IC) inoculation. We tried to re-adapt this brain virus to lung of weanling hamster. The brain passaged virus was inoculated into weanling hamster intramuscularly. Again, the titer of the virus in lung was also increased by continuous passage of this virus. This facts could regarded as adaptation to new environment in which the virus proliferates. To identity the virus passaged in hamster with Maaji virus, both of the virus passaged in hamster brain and lung were compared with Maaji virus (MAA-I) and Hantaan virus (HTN 76-118) by means of restriction fragment length polymorphism (RFLP) and slingle strand conformation polymophism (SSCP). As a result, we conclude that Maaji virus could be adapted successfully to weanling hamster through this passage strategy. Utilizing this adapted Maaji virus strain, hamster model is able to be used for challenge test in hantaviral vaccinology and further experiments utilizing hamster system as a rather available and convenient lab animal are expected.

• 융합보안논문지
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• 제5권1호
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• pp.53-62
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• 2005

virus 방역 체계 관리는 network infra 구조관리, traffic 소통경로관리, 방역 zone 설정, gateway구간 방역관리이다. 본 논문은 일반적인 방역체계 구조의 성격과 취약점을 진단하고 이를 개선할 수 있는 대책으로서 개선된 방역체계를 설계하였다. 또한 설계된 방역체계와 configuration, mechanism하에서는 어느 정도의 개선효과 나타나고 있는지 분석하였다. 개선된 다단계 방역체계 하에서는 gateway 단계에서 불필요한 mail을 걸러줌으로서 server에 주는 부하는 감소하며 virus wall상의 CPU 부하의 감소와 virus 치료율의 상승으로 송신 적체 건수는 감소하고 시스템 process수는 증대하고 있다.

• 한국잠사곤충학회지
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• 11호
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• pp.59-62
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• 1970

1. 한국에서 작잠농병을 발생시키는 주된 Virus는 핵질다색체 Virus 이다. 2. Virus 묶음은 봉입체단백직의 분자구조내에 함입되어있다. 3. 봉입체단백질에 존재하는 Virus묶음은 평균4개의 간상형 Virus입자로 되어있으며 이를 싸고있는 막은 두개로 되어있다. 4. 봉입체단백질내에 존재하는 Virus묶음은 질서와 균형있게 배열된 것이 아니고 되는데로 산재해 있는 것 같다. 5. Virus입자와 봉입예단백질은 전염된 세포의 염색체에서 형성된 소위 "Virogenic stroma"에서 생겨진것이다.

• Perspectives in Nursing Science
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• 제7권1호
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• pp.50-54
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• 2010

The studies of M. Colombo (1989) and W. Lange (1992) showed that 30~40% of people became chronic after suffering from hepatitis B virus (HBV) and C virus (HCV) infection, and about 50% of the chronic cases transformed into primary liver cancer. There have been few studies done in Mongolia on hepatitis infection among health professionals, particularly in nurses. In a study done by Chimedsuren (8), the study showed that 19.4% of people with identified surface hepatitis B antigen (HBsAg) and antibodies to hepatitis C virus and 8% of people with the identified nucleotide of RNA for the hepatitis C virus (polymerase chain reaction) had an acute form of hepatitis C. Studies on the hepatitis virus genome damaging effect on liver cells showed that genotype 8 (A, B, C, D, E, F, G, TTV) had the most damaging effect on liver cells (Hahn and Faeka, 2007). Several studies have shown a relationship between hepatitis B virus infection and a lack of compliance regarding safety regulations and rules by medical personnel. Results of a study from the Maternal and Child Health Research Center showed that tests done to detect hepatitis B virus antigen and antibodies to C virus did not reveal anything. Both antigen and antibodies in 69% cases did not show, and separately, B virus and antibodies to hepatitis C virus were identified in 13% and 9%, respectively. Results of the tests taken from health personnel in Shastin Central Hospital showed that in 76% of the cases, the B virus antigen with C virus antibodies was not identified. In 8% of the cases, the B virus antigen was present on its own. The combination of B the virus antigen and C virus antibodies were present in 8% of nurses and doctors, respectively. 82% of the cases had negative results for the detection of a combination of B virus antigen and C virus antibodies taken from health personnel from the State Central Clinical Hospital whereas the B virus antigen and C virus antibodies by themselves were present in 7% and 14% of the cases, respectively. Combined cases of the B virus antigen and C virus antibodies were identified in 4% of the personnel. Results of the tests taken from the health personnel in the Hospital of the Ministry of Justice and Internal Affairs showed that in 79% of the cases, the B virus antigen with C virus antibodies were not identified. Separately, the B virus and antibodies to hepatitis C virus were identified in 8% and 13% of the cases, respectively.

• Journal of Microbiology
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• 제40권1호
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• pp.1-10
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• 2002

At the dose of 1000 p.f.u. per mouse,100% mortality occurred in mice inoculated with wild-type pseudorabies virus (PrV). In contrast, upon stable deletion of 10 bp nucleotides at the Bsrl site within the TK gene, PrV was rendered to be completely apathogenic. The deletion also caused the virus to be less capable of replicating in respiratory as well as in nervous system tissues. Although animals were exposed to high titers of TK-deleted PrVs, the virus failed to replicate to a high titer as compared to the pathogenic parental virus. In contrast to previous studies the deletion in the TK gene did not prevent the virus from establishing latency. Upon immunosuppression, the latent virus? however, reactivated but replicated at low titers. Interestingly, TK-deleted virus established latency and reactivation, that are occurred only in trigeminal ganglia and the cerebrums and no other tissues involved. Following reactivation, there was no indication of virus shedding in respiratory tissues as confirmed by virus isolation and polymerase chain reaction (PCR) technique targeting at the gB gene of PrV, The non-pathogenic virus with non-shedding characteristics, upon reactivation of the latent virus, would be the important feature of a live virus vaccine candidate.

• 한국사이버테러정보전학회:학술대회논문집
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• 한국사이버테러정보전학회 2004년도 제1회 춘계학술발표대회
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• pp.187-198
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• 2004

virus방역 체계 관리는 network infra구조관리, traffic소통경로관리. 방역 zone설정, gateway구간 방역관리이다. 본 논문은 일반적인 방역체계 구조의 성격과 취약점을 진단하고 이를 개선할 수 있는 대책으로서 개선된 방역체계를 설계하였다. 또한 설계된 방역체계와 configuration, mechanism하에서는 어느 정도의 개선효과 나타나고 있는지 분석하였다. 개선된 다단계 방역체계 하에서는 gateway 단계에서 불필요한 mail을 걸러주므로서 server에 주는 부하는 감소하며 virus wall상의 CPU부하의 감소와 virus 치료율의 상승으로 송신 적체 건수는 감소하고 시스템 process수는 증대하고있다.

• 한국식물병리학회지
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• 제3권4호
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• pp.261-269
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• 1987

포장에서 고추에 황화, 엽맥록대 그리고 엽맥주름을 일으키는 바이러스가 분리되었다. 이 바이러스는 PVY항혈청과 ELISA 검정에서 반응하였으나 CMV, TMV, TBRV, AMV, TSWV, TEV, PMV, TRSV와는 반응하지 않았다. 바이러스 입자의 전자현미경 검경 결과 길이가 750-760nm의 사상형 입자였다. 이 바이러스는 진딧물에 의해 쉽게 전염되었으며 기주범위는 PVY와 비슷하였으나 Chemopodium amaranticolor와 C. quinoa에 전신 감염을 일으켰다.

• The Plant Pathology Journal
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• 제20권3호
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• pp.206-211
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• 2004

Transgenic Nicotiana benthamiana plants harboring coat protein (CP) gene of Zucchini green mottle mosaic virus (ZGMMV) were generated for virus-resistant screening and complementation analysis of related viruses for environmental safety assessment (SA) of living modified organism (LMO) purposes. Transformation of leaf disc of N.benthamiana was performed by using Agrobacterium-mediated method and the pZGC-PPGA748 containing the ZGMMV CP and NPTII genes. Two kinds of transgenic homozygous groups, virus-resistant and virus-susceptible N.benthamiana lines, were obtained by screening of challenging homologous virus for Tl generations. These two pathologically different lines can be useful for host-virus interactions and LMO environmental SA.

• The Plant Pathology Journal
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• 제20권2호
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• pp.147-154
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• 2004

A simple and reliable procedure for RT-PCR detection of Apple stem pitting virus (ASPV), Cherry rasp leaf virus (CRLV), and Cherry necrotic rusty mottle virus (CNRMV) was developed. Two virus specific primer sets for each virus were found to specifically detect each virus among fourteen sets of designed oligonucleotide primers. Total RNAs extracted from healthy and from ASPV-,CRLV- and CNRMV-infected plant tissues were used to synthesize cDNA using oligo dT primer and then amplified by virus-specific primers for each virus. Each primer specifically amplified DNA fragments of 578 bp and 306 bp products for ASPV (prAS CP-C and prAS CP-N primers, respectively); 697 bp and 429 bp products for CRLV (prCR4 and prCR5-JQ3D3 primers, respectively); and 370 bp and 257 bp products for CNRMV (prCN4 and prCN6-NEG 1 primers, respec-tively) by RT-PCR. DNA sequencing of amplified DNA fragments confirmed the nature of each amplified DNA. Altogether, these results suggest that these virus specific primer sets can specifically amplify viral sequences in infected tissues and thus indicate that they can be used for specific detection of each virus.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.146.1-146
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• 2003

Transgenic Nicotiana benthmiana plants harboring and expressing coat protein (CP) gene of Zucchini green mottle mosaic virus (ZGMMV) were generated for both virus-resistant screening and complementation analysis of related viruses and environmental safety assessment (SA) of living modified organism (LMO) purposes. Transformation of leaf disc of N. benthamiana was performed using Agrobacterium-mediated method and the pZGCPPGA748 containing the ZGMMV CP and NPTII genes. Two kinds of transgenic homozygous groups, virus-resistant and -susceptible lines, were obtained by screening of challenging homologous virus for T1 generations. Complementation of CP-deficient related virus was analyzed using the susceptible line of ZGMMV. These two pathologically different lines can be useful for host-virus interactions and LMO environmental SA.