The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

RT-PCR Detection of Three Non-reported Fruit Tree Viruses Useful for Quarantine Purpose in Korea

  • Park, Mi-Ri (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Kook-Hyung (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

A simple and reliable procedure for RT-PCR detection of Apple stem pitting virus (ASPV), Cherry rasp leaf virus (CRLV), and Cherry necrotic rusty mottle virus (CNRMV) was developed. Two virus specific primer sets for each virus were found to specifically detect each virus among fourteen sets of designed oligonucleotide primers. Total RNAs extracted from healthy and from ASPV-,CRLV- and CNRMV-infected plant tissues were used to synthesize cDNA using oligo dT primer and then amplified by virus-specific primers for each virus. Each primer specifically amplified DNA fragments of 578 bp and 306 bp products for ASPV (prAS CP-C and prAS CP-N primers, respectively); 697 bp and 429 bp products for CRLV (prCR4 and prCR5-JQ3D3 primers, respectively); and 370 bp and 257 bp products for CNRMV (prCN4 and prCN6-NEG 1 primers, respec-tively) by RT-PCR. DNA sequencing of amplified DNA fragments confirmed the nature of each amplified DNA. Altogether, these results suggest that these virus specific primer sets can specifically amplify viral sequences in infected tissues and thus indicate that they can be used for specific detection of each virus.

Keywords

References

  1. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D. J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402 https://doi.org/10.1093/nar/25.17.3389
  2. Cropley, R. 1968. Fruit tree viruses in Britain-our knowlege and ignorance. Scientific Horticulture 20:95-100
  3. Desvignes,J. C. 1999. Virus diseases offruit trees. Ctifl, Paris
  4. Hadidi, A., Levy, L. and Podleckis, E. V. 1995. Polymerase chain reaction technology in plant pathology. In 'Molecular methods in plant pathology' (R. P. Singh, and U. S. Singh, Eds.), pp. 167-187. CRC Press, Inc., Boca Raton, Florida
  5. Hansen, A. J., Nyland, G., McElroy, F. D. and Stace-Smith, R. 1974. Origin, cause, host range, and spread of cherry raspleaf disease in North America. Phytopathology 64
  6. Ito, T., Ieki, H. and Ozaki, K. 2002. Simultaneous detection of six citrus viroids and Apple stem grooving virus from citrus plants by multiplex reverse transcription polymerase chain reaction. J. Viml. Methods 106:235-239 https://doi.org/10.1016/S0166-0934(02)00147-7
  7. James, D., Howell, W. E. and Mink, G. I. 2001. Molecular evidence of the relationship between a virus associated with flat apple disease and Cherry rasp leafvirus as determined by RT-PCR. Plant Dis. 85:47-52 https://doi.org/10.1094/PDIS.2001.85.1.47
  8. James, D. and Upton, C. 2002. Nucleotide sequence analysis of RNA-2 of a flat apple isolate of Cherry rasp leaf virus with regions showing greater identity to animal picomaviruses than to related plant viruses. Arch. Viral. 147: 1631-1641 https://doi.org/10.1007/s00705-002-0833-3
  9. Jung, H. W., Yun, W. S., Oh, M. H., Hahn, Y. I. and Kim, K.-H. 2002. Characterization of Tobacco mosaic virus isolated from potato showing yellow leaf mosaic and stunting symptoms in Korea. Plant Dis. 86:112-117 https://doi.org/10.1094/PDIS.2002.86.2.112
  10. Kim, K.-H. 1999. Recent development in detection and identification of fruit tree viruses. Plant Pathol. J. 15: 199-209
  11. Kim, S. M., Lee, J. M., Yim, K. O., Oh, M. H., Park, J. W. and Kim, K.-H. 2003. Nucleotide sequences of two Korean isolates of Cucumber green mottle mosaic virus. Mol. Cells 16:407-412
  12. Kokko, H. I., Kivineva, M. and Karenlampi, S. O. 1996. Singlestep immunocapture RT-PCR in the detection of raspberry bushy dwarf virus. Biotechniques 20:842-846
  13. Korschineck, I., Himmler, G., Sagl, R., Steinkellner, H. and Katinger, H. W. 1991. A PCR membrane spot assay for the detection of plum pox virus RNA in bark of infected trees. J. Viral. Methods 31:139-145. https://doi.org/10.1016/0166-0934(91)90152-P
  14. Kundu, J. K. 2003. A rapid and effective RNA release procedure for virus detection in woody plants by reverse transcriptionpolymerase chain reaction. Acta Virol. 47: 147-151
  15. Martelli, G. P. and Jelkmann, W. 1998. Foveavirus, a new plant virus genus. Arch. Virol. 143:1245-1249 https://doi.org/10.1007/s007050050372
  16. Nolasco, G., de Bias, C., Torres, V. and Ponz, F. 1993. A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens. J. Virol. Methods 45:201-218 https://doi.org/10.1016/0166-0934(93)90104-Y
  17. Nyland, G. 1976. Cherry rasp leaf. Agriculture, Agricultural handbook, 437
  18. Nyland, G., Lownsbery, B. F., Lowe, S. K. and Mitchell, J. F. 1969. The transmission of cherry rasp leaf virus by Xiphinema americanum. Phytopathology 59: 1111-1112
  19. Romaine, C. P. and Schlagnhaufer, B. 1995. PCR analysis of the viral complex associated with La France disease of Agaricus bisporus. Appl. Environ. Microbial. 61 :2322-2325
  20. Rosner, A., Maslenin, L. and Spiegel, S. 1997. The use of short and long PCR products for improved detection of prunus necrotic ringspot virus in woody plants. J. Viral. Methods 67:135-141 https://doi.org/10.1016/S0166-0934(97)00088-8
  21. Rott, M. E. and Jelkmann, W. 2001. Complete nucleotide sequence of cherry necrotic rusty mottle virus. Arch. Virol. 146:395-401 https://doi.org/10.1007/s007050170184
  22. Singh, R. P., Nie, X., Singh, M., Coffin, R. and Duplessis, P. 2002. Sodium sulphite inhibition of potato and cherry polyphenolics in nucleic acid extraction for virus detection by RT-PCR. J. Viral. Methods 99:123-131 https://doi.org/10.1016/S0166-0934(01)00391-3
  23. Spiegel, S., Scott, S. W., Bowman, V. V., Tam, Y, Galiakparov, N. N. and Rosner, A. 1994. Improved detection of prunus necrotic ringspot virus by the polymerase chain reaction. Eur. J. Plant Pathol. 102:681-685 https://doi.org/10.1007/BF01877249
  24. Stouffer, R. F. and Fridund, P. R. 1989. Apple stem pitting. In 'Virus and Viruslike Diseases of Pome Fruits and Simulating Noninfectious Disorders'. Cooperative Extension College of Agriculture and Home Economics Washington State University
  25. Sugieda, M., Nagaoka, H., Kakishima, Y., Ohshita, T., Nakamura, S. and Nakajima, S. 1998. Detection of Norwalk-like virus genes in the caecum contents of pigs. Arch. Virol. 143: 1215-1221 https://doi.org/10.1007/s007050050369
  26. Vitushkina, M., Fechiner, B., Agranovsky, A. and Jelkmann, W. 1994. Development of an RT-PCR for the detection of little virus and characterization of some isolates occurring in Europe. Eur. J. Plant Pathol. 103:803-808 https://doi.org/10.1023/A:1008679224682
  27. Wadley, B. and Lipman, D. J. 1976. 'Rusty mottle group.' Agriculture Research Service United State Department of Agriculture (R. M. Gilmer, J. D. Moor, G. Nyland, M. F. Welsh, and T. Pine, Eds.), Washington
  28. Yanas, H., Milk, G. I., Sawamura, K. and Yamaguchi, A. 1990. Apple topworking disease. In "American Phytopathology" (A. L. Jones, and H. S. Aldwinckle, Eds.), pp. 451-456. 79 vols
  29. http://www.boku.ac.at/iam/pbiotech/phytopath/d_slr.html

Cited by

  1. RT-PCR Detection of Five Quarantine Plant RNA Viruses Belonging to Potyand Tospoviruses vol.27, pp.3, 2011, https://doi.org/10.5423/PPJ.2011.27.3.291
  2. Development of RT-PCR and Nested PCR for Detecting Four Quarantine Plant Viruses Belonging to Nepovirus vol.19, pp.3, 2013, https://doi.org/10.5423/RPD.2013.19.3.220
  3. Development of PCR Diagnosis System for Plant Quarantine Seed-borne Wheat Streak Mosaic Virus vol.49, pp.2, 2013, https://doi.org/10.7845/kjm.2013.3013
  4. Development and of Diagnostic System for Detection of Cowpea chlorotic mottle virus using by Nested PCR vol.41, pp.4, 2014, https://doi.org/10.7744/cnujas.2014.41.4.335
  5. Detection of Carnation necrotic fleck virus and Carnation ringspot virus Using RT-PCR vol.19, pp.1, 2013, https://doi.org/10.5423/RPD.2013.19.1.036
  6. Development and Practical Use of RT-PCR for Seed-transmitted Prune dwarf virus in Quarantine vol.30, pp.2, 2014, https://doi.org/10.5423/PPJ.NT.10.2013.0099
  7. Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR vol.30, pp.4, 2014, https://doi.org/10.5423/PPJ.NT.05.2014.0041
  8. Development of PCR Diagnostic System for Detection of the Seed-Transmitted Tobacco Ringspot Virus in Quarantine vol.55, pp.2, 2015, https://doi.org/10.1007/s12088-015-0518-8
  9. Development of a PCR Diagnostic System for Detecting Andean Potato Mottle Virus Associated with Potato Quarantine in Korea vol.92, pp.4, 2015, https://doi.org/10.1007/s12230-015-9468-2
  10. Development and Optimization of a Reverse Transcription Hemi-Nested PCR Primer for the Detection of Potato Mop-Top Virus at Quarantine Inspection Sites in Korea vol.57, pp.2, 2017, https://doi.org/10.1007/s12088-016-0623-3