The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Virus Resistant and Susceptible Transgenic Nicotiana benthamiana Plants Expressing Coat Protein Gene of Zucchini green mottle mosaic virus for LMO Safety Assessment

  • Kim, Min-Jea (Plant Virus GenBank,Division of Environmental and Life Sciences, Seoul Womens University) ;
  • Choi, Sun-Hee (Plant Virus GenBank,Division of Environmental and Life Sciences, Seoul Womens University) ;
  • Kim, Tae-Sung (Biological Resources Division, Biodiversity Research Department, National Institute of Environmental Research) ;
  • Park, Min-Hye (Plant Virus GenBank,Division of Environmental and Life Sciences, Seoul Womens University) ;
  • Lim, Hee-Rae (Plant Virus GenBank,Division of Environmental and Life Sciences, Seoul Womens University) ;
  • Oh, Kyung-Hee (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Tae-San (National Institute of Agricultural Biotechnology, RDA) ;
  • Lee, Min-Hyo (School of Agricultural Biotechnology, Seoul National University) ;
  • Ryu, Ki-Hyun (Plant Virus GenBank,Division of Environmental and Life Sciences, Seoul Womens University)
  • Published : 2004.09.01
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Abstract

Transgenic Nicotiana benthamiana plants harboring coat protein (CP) gene of Zucchini green mottle mosaic virus (ZGMMV) were generated for virus-resistant screening and complementation analysis of related viruses for environmental safety assessment (SA) of living modified organism (LMO) purposes. Transformation of leaf disc of N.benthamiana was performed by using Agrobacterium-mediated method and the pZGC-PPGA748 containing the ZGMMV CP and NPTII genes. Two kinds of transgenic homozygous groups, virus-resistant and virus-susceptible N.benthamiana lines, were obtained by screening of challenging homologous virus for Tl generations. These two pathologically different lines can be useful for host-virus interactions and LMO environmental SA.

Keywords

References

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