• 대한한방부인과학회지
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• 제18권1호
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• pp.169-180
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• 2005

Objective : Spatholobus Suberectus Dunn stems, Chinese vine plants, have been used for the relief of menstrual disorders and rheumatic arthralgia. In this study, we investigated the antitumor effect of Spatholobus Suberectus Dunn on cervical cancer in vitro. Methods : HeLA cervical cancer cell lines were used as targets. We examined the effect of water extract from Spatholobus Suberectus Dunn on cell proliferation, cell cycle regulation and cell cycle-regulating gene expression. Further, we investigated the apoptotic effects of Spatholobus Suberectus Dunn on cervical cancer cell lines. Results : Spatholobus Suberectus Dunn significantly inhibited the proliferation of cervical cancer cell lines in a dose-dependent and time dependent manner. Fluorescence activated cell sorter (FACS) analysis indicated that Spatholobus Suberectus Dunn induced G1 cell cycle arrest. Spatholobus Suberectus Dunn enhanced the expression of $p21^{waf1}$ and $p27^{kip1}$ with cell cycle arrest. Further, Spatholobus Suberectus Dunn stimulated apoptosis via caspase3 pathway. Conclusions : These findings suggest that Spatholobus Suberectus Dunn is a candidate agent for the treatment of cervical cancer. p21waf1 and $p21^{waf1}$ and $p27^{kip1}$ may play an important role in Spatholobus Suberectus Dunn-induced cell cycle arrest and cell growth inhibition.

• Journal of Applied Biological Chemistry
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• 제65권4호
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• pp.277-286
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• 2022

Schizophragma hydrangeoides (S. hydrangeoides) is a vine endogenous to Jeju Island and Ulleungdo, where it grows attached to the foothills and rock surfaces. Previous research has mostly focused on the whitening effect of S. hydrangeoides leaf extract. In this study, we investigated S. hydrangeoides leaf extract further, and detected four phytochemicals in the extract: chlorogenic acid, quercetin-3-O-glucosyl-(1-2)-rhamnoside, quercetin-3-O-xylosyl-(1-2)-rhamnoside, and quercitrin. We pretreated human dermal fibroblast (HDFn) cells with previously established concentrations of the four compounds for 1 h before ultraviolet A (UVA) irradiation. Among the four compounds, quercetin-3-O-glucosyl-(1-2)-rhamnoside (Q-3-GR) best inhibited matrix metalloproteinase-1 (MMP-1) levels. Thus, we investigated the protective effects of Q-3-GR on photoaging and its underlying mechanisms. Q-3-GR significantly reduced MMP-1 production and inhibited MMP-1 protein expression in UVA-irradiated HDFn cells. Furthermore, Q-3-GR increased procollagen type I production and protein expression. Q-3-GR exerted its anti-photoaging effects by downregulating the mitogen-activated protein kinase/ activator protein-1 signaling pathway, and upregulating the transforming growth factor-β/Smad signaling pathway. Thus, S. hydrangeoides leaf-derived Q-3-GR is a potential potent cosmetic ingredient for UV-induced skin aging.

• 한국수산과학회지
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• 제48권1호
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• pp.96-103
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• 2015

The effects of $CO_2$ concentration, nutrient levels, and irradiance on the growth of germlings and juveniles, and on the photosynthesis of adults were examined in a green tide alga, Ulva australis. We used a factorial experimental design with two $CO_2$ concentrations (380 and 750 ppm), two nutrient levels (control and PES medium), and two irradiance levels (50 and $100{\mu}mol$ photons $m^{-2}s^{-1}$). Germlings grew best ($664.15{\pm}61.45{\mu}m$ in length) under conditions of 750 ppm, PES, and $100{\mu}mol$ photons $m^{-2}s^{-1}$ after 10 days in culture. Relative growth rates (RGR) of the juveniles were greatest (4.41% $day^{-1}$) under conditions of 750 ppm, PES, and $50{\mu}mol$ photons $m^{-2}s^{-1}$ after 5 days in culture. Photosynthetic efficiency ($F_v/F_m$) of the adult discs was $0.73{\pm}0.05$ before the experiment and reached a maximum ($0.83{\pm}0.01$) under conditions of 750 ppm, control, and $50{\mu}mol$ photons $m^{-2}s^{-1}$ after 5 days in culture. Growth (germlings and juveniles) and photosynthesis (adult discs) of Ulva australis increased when $CO_2$ levels were 750 ppm. Additionally, the optimal irradiance for growth and photosynthesis differed among stages, wherein germlings grew best at $100{\mu}mol$ photons $m^{-2}s^{-1}$, juveniles grew best at $50{\mu}mol$ photons $m^{-2}s^{-1}$, and adults photosynthesized most at $50{\mu}mol$ photons $m^{-2}s^{-1}$. The performance of Ulva australis at all examined life stages was enhanced under the PES nutrient treatment. In conclusion, the physiological responses of U. australis to varying $CO_2$, nutrient, and irradiance levels differed slightly among life stages. However, growth and photosynthesis always increased with elevated $CO_2$ and nutrient concentrations. These results indicate that U. australis green tide blooms might occur more frequently in coastal areas if $CO_2$ and nutrient concentrations increase.

• 생물환경조절학회지
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• 제21권3호
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• pp.186-191
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• 2012

참외에서의 관비재배기술 개발을 위하여 배양액의 공급횟수가 토양의 EC 변화, 질소와 칼슘 집적, 덩굴의 생장, 과실의 수량 및 품질에 미치는 영향을 조사하였다. 관비재배는 관행에 비하여 토양 EC가 $0.8dS{\cdot}m^{-1}$까지 증가 하였으나 관행재배는 $0.68dS{\cdot}m^{-1}$에서 $0.2dS{\cdot}m^{-1}$로 낮아졌다. 정식 후 20일 된 식물체의 덩굴길이는 큰 차이가 없었으나 건물중은 관비재배구가 증가하였는데 2회(오전 9시와 오후 6시, 900L/1,000포기, 매일)처리구가 현저히 많았다. 토양 내 질소함량에는 큰 차이가 없었으나 칼슘은 관비재배구가 현저히 높았다. 관비는 관행재배에 비하여 참외과실의 상품(商品)수량이 증가하였는데 1일 1회(오전 9시)보다 2회 관비재배구가 더욱 많았고 기형과율은 관비재배구가 감소하였다. 1개 과실의 크기와 당도에는 차이가 없었다. 저장기간 동안 과실의 중량 감소는 2회 관비재배구가 현저히 많았다.

• 생물환경조절학회지
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• 제24권3호
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• pp.252-257
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• 2015

노지 포도재배는 강우에 직접 노출되어 열과와 병 발생이 많아진다. 그래서 도입된 것이 간이 비가림 재배이지만, 이것 역시 열과와 병 발생을 예방하는 데는 한계가 있다. 따라서 간이비가림 시설의 문제점을 보완하기 위해서 완전하게 강우를 차단하면서 자연적인 환기가 가능한 비가림 시설을 개발하여 적설하중과 풍하중을 내재 해 규격에 맞추었다. 개선된 비가림 시설의 특성을 검증하기 위해서 $100m^2$ 규모의 시설을 설치한 다음 관행 비가림 시설과 시설 내외부 온도 분포와 그에 따른 포도캠벨얼리의 품질, 갈반병 발생 및 열과율 등을 조사하여 비교하였다. 관행 비가림 시설과 개선된 비가림 시설의 온도 조사결과 외기온이 $34^{\circ}C$ 이상일 경우 시설 내부온도가 관행 최대 $40.7^{\circ}C$, 개선 시설은 $37.4^{\circ}C$로 측정되어 개선 시설이 3정도 낮았고, $32^{\circ}C$ 이하에서는 관행과 개선 시설 간에 온도 차이가 없었다. 과실 품질은 개선된 비가림 시설이 관행 비가림 시설보다 당도와 상품과율이 높았다. 개선된 비가림 시설이 관행에 비해 열과율과 갈반병 발병율이 현저히 낮았다.

• 한국자원식물학회지
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• 제22권4호
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• pp.299-303
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• 2009

넓은잎큰조롱의 두둑높이에 따른 생산성 및 품질향상을 위하여 재배법 확립의 기초자료로 제공하고자 두둑높이를 달리하여 2005년부터 3년간 시험한 결과, 만장, 엽장, 엽폭, 엽수, 엽록소함량, 경엽 건물중은 두둑높이가 높을수록 양적인 증가를 가져왔으나, 경태와 분지수는 두둑높이가 낮을수록 굵거나, 많은 경향이었다. 근수와 근장은 두둑높이 20 cm까지는 두둑높이가 높을수록 많거나, 길었으나, 20 cm 이상의 두둑높이에서는 생육량이 감소하였으며, 근태는 두둑높이가 낮을수록 굵어지는 경향이었다. 비상품근 중 세근은 두둑높이 20 cm에서 다소 많았으며, 부패근은 두둑높이가 낮을수록 많은 경향이었고, 근수량은 두둑높이 20 cm내지 30 cm 까지는 두둑높이가 높을수록 증수되어 두둑높이 10 cm의 515 kg/10a 대비 3%, 11% 증수되었다.

• 한국조경학회지
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• 제33권2호
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• pp.32-47
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• 2005

This study was carried out to investigate and propose a green-wall system with evergreen plants for urban greening of Tongyeong City. To achieve these goals, the requirements and possibilities for wall greening were investigated and evaluated considering the location, topography, and climate of Tongyeong City. Existing walls were analyzed and then a suitable green wall system is proposed. Tongyeong City and its 151 islands covers the central and the southern parts of the Goseong peninsula. Most of the land is covered with hills and mountains; $43.9\%$ of the land area has a slope greater than $15\%$ and most hills and mountains near the urban area have a slope of more than $30\%$. As a result of the topographical properties, concrete retaining walls can often be seen along the streets in urbanized areas. These retaining walls are not only unattractive, but they also create environmental problems, and thus should be replaced with native evergreen plants. Options for replacing the retaining walls include evergreen vine-plants such as Hedaa spp. and Euonymus radicans, but native evergreen shrubs such as Pittosporum tobira, Nandina domestica, Raphiolepis umbellata, Ilex cornuta, flex crenata, Fatsia japonic, and Aucuba japonica may be a more attractive option. Current wall conditions are unsuitable for planting vines, therefore, a reservoir-drainage-type plant box filled with a light artificial substrate is required for greening these concrete retaining walls. These might be irrigated in the dry season and fertilized annually by an appropriate system. These plant boxes could be attached along the entire walls. An experiment investigating effects of substrates and bark-chip mulching on the growth of Hedera spp. showed that the mixture of cerasoil and field soil(v/v, 4:6) was superior to field soil alone and to the mixture of perlite small grain, large grain, and field soil(v/v/v, 2:2:6). Bark-chip mulching tended to increase the growth of Hedera spp..

• 한국약용작물학회지
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• 제16권2호
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• pp.69-73
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• 2008

넓은잎큰조롱의 경운깊이에 따른 생산성 및 품질향상을 위한 구명하기 위하여 경운깊이를 달리하여 2005년부터 2년간 시험한 결과, 만장은 경운깊이가 얕을수록 길어 경운깊이가 30 cm 일 때 685 cm 인데 비하여 경운깊이 10 cm 일 때 59 cm 가 더 길었으며, 경태와 건물중도 같은 경향으로 굵거나 무거웠다. 엽장, 엽폭, 엽중은 경운깊이가 얕을수록 양적인 생육을 보였으나, 엽록소 함량은 경운깊이가 깊을수록 증가하였다. 근수와 근장은 경운깊이가 깊을수록 많거나 길어지는 경향이었으나, 근태와 부패근은 경운깊이가 얕을수록 굵거나 많은 경향이었고, 근수량은 경운깊이가 깊을수록 많은 경향으로 경운깊이가 10 cm 일 때 $622\;kg/1000m^2$인데 비하여 20 cm 이상으로 경운할 때 $7{\sim}9%$ 증수되었다.

• 생태와환경
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• 제52권2호
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• pp.171-177
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• 2019

Smart farm is a breakthrough technology that can maximize crop productivity and economy through efficient utilization of space regardless of external environmental factors. This study was conducted to investigate the optimal growth and physiological conditions of Chinese matrimony vine (Lycium chinense) with LED light sources in a smart farm. The light source was composed of red+blue and red+blue+white mixed light using a LED system. In the red+blue mixed light, red and blue colored LEDs were mixed at ratios of 1:1, 2:1, 5:1, and 10:1, with duty ratios varied to 100%, 99%, and 97%. The experimental results showed that the photosynthetic rate according to the types of light sources did not show statistically significant differences. Meanwhile, the photosynthetic rate according to the mixed ratio of the red and the blue light was highest with the red light and blue LED ratio of 1:1 while the water use efficiency was highest with the red and blue LED ratio of 2:1. The photosynthetic rate according to duty ratio was highest with the duty ratio of 99% under the mixed light condition of red+blue+white whereas the water use efficiency was highest with the duty ratio of 97% under the mixed light of red+blue LED. The results indicate that the light source and light quality for the optimal growth of Lycium chinense in the smart farm using the LED system are the mixed light of red+blue (1:1) and the duty ratio of 97%.

• 생물환경조절학회지
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• 제22권3호
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• pp.241-247
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• 2013

돌나물 유전자원의 기내 활성보존을 위하여, 10mm 크기의 기내배양 shoot를 agar, Gelrite, ABA 및 sucrose 농도를 달리한 MS 배지에 치상하여, $4^{\circ}C$와 $25^{\circ}C$에서 계대배양 없이 보존하였다. 배지는 $0.2mg{\cdot}L^{-1}$ BA를 기본으로 첨가하였고, agar와 Gelrite 배지에는 5% sucrose, ABA 배지에는 5% sucrose와 1.2% agar, sucrose 배지에는 1.2% agar를 각각 첨가하였다. 상온 활성보존($25^{\circ}C$)에서 sucrose와 Gelrite 배지는 생장억제 효과가 거의 없었고, $0.2mg{\cdot}L^{-1}$ BA + $10mg{\cdot}L^{-1}$ ABA + 1.2% agar, 또는 $0.2mg{\cdot}L^{-1}$ BA + 1.6% agar를 첨가한 배지가 효과적이었으며, 계대배양 없이 10개월까지 활성보존이 가능하였다. 저온 활성보존($4^{\circ}C$)에서 12개월후 생존율은 모든 배지에서 100%였으며, $10mg{\cdot}L^{-1}$ ABA, 또는 6% sucrose 첨가배지에서 계대배양 없이 18개월 이상의 활성보존이 가능하였다. 특히 고농도 sucrose 배지는 저온 활성보존($4^{\circ}C$)에서 돌나물 유전자원의 장기간 활성유지에 가장 효과적이었다.