Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Protective effects of quercetin-3-glucosyl-(1-2)-rhamnoside from Schizophragma hydrangeoides leaves on ultraviolet A-induced photoaging in human dermal fibroblasts

  • So Yeon, Oh (Biodiversity Research Institute, Jeju Technopark) ;
  • Sung Chun, Kim (Biodiversity Research Institute, Jeju Technopark) ;
  • Ho Bong, Hyun (Biodiversity Research Institute, Jeju Technopark) ;
  • Hyejin, Hyeon (Biodiversity Research Institute, Jeju Technopark) ;
  • Boram, Go (Biodiversity Research Institute, Jeju Technopark) ;
  • Yong-Hwan, Jung (Biodiversity Research Institute, Jeju Technopark) ;
  • Young-Min, Ham (Biodiversity Research Institute, Jeju Technopark)
  • Received : 2022.09.30
  • Accepted : 2022.10.21
  • Published : 2022.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Schizophragma hydrangeoides (S. hydrangeoides) is a vine endogenous to Jeju Island and Ulleungdo, where it grows attached to the foothills and rock surfaces. Previous research has mostly focused on the whitening effect of S. hydrangeoides leaf extract. In this study, we investigated S. hydrangeoides leaf extract further, and detected four phytochemicals in the extract: chlorogenic acid, quercetin-3-O-glucosyl-(1-2)-rhamnoside, quercetin-3-O-xylosyl-(1-2)-rhamnoside, and quercitrin. We pretreated human dermal fibroblast (HDFn) cells with previously established concentrations of the four compounds for 1 h before ultraviolet A (UVA) irradiation. Among the four compounds, quercetin-3-O-glucosyl-(1-2)-rhamnoside (Q-3-GR) best inhibited matrix metalloproteinase-1 (MMP-1) levels. Thus, we investigated the protective effects of Q-3-GR on photoaging and its underlying mechanisms. Q-3-GR significantly reduced MMP-1 production and inhibited MMP-1 protein expression in UVA-irradiated HDFn cells. Furthermore, Q-3-GR increased procollagen type I production and protein expression. Q-3-GR exerted its anti-photoaging effects by downregulating the mitogen-activated protein kinase/ activator protein-1 signaling pathway, and upregulating the transforming growth factor-β/Smad signaling pathway. Thus, S. hydrangeoides leaf-derived Q-3-GR is a potential potent cosmetic ingredient for UV-induced skin aging.

Keywords

Acknowledgement

This research was financially supported by the Ministry of Trade, Industry and Energy, Korea, under the "Regional Innovation Cluster Development Program (R&D, P0015361)" supervised by the Korea Institute for Advancement of Technology (KIAT).

References

  1. Lee YR, Noh EM, Jeong EY, Yun SK, Jeong YJ, Kim JH, Kwon KB, Kim BS, Lee SH, Park CS, Kim JS (2009) Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-κB pathway in human dermal fibroblasts. Exp Mol Med 41: 548-554. doi: 10.3858/emm.2009.41.8.060 
  2. Tomasello B, Malfa GA, Acquaviva R, Mantia AL, Giacomo CD (2022) Phytocomplex of a standardized extract from red orange (Citrus sinensis L. Osbeck) against photoaging. Cells 11: 1447. doi: 10.3390/cells11091447 
  3. Rittie L, Fisher GJ (2002) UV-light-induced signal cascades and skin aging. Ageing Res Rev 1: 705-720. doi: 10.1016/S1568-1637(02)00024-7 
  4. Zhang H, Zhang Y, Tong X, Tong X, Gao L, Kang L, Zeng J (2021) The Epigenetic Modification in the Pathogenesis of Skin Photoaging. COJ Nurse Healthcare 7: 760-768. doi: 10.31031/COJNH.2021.07.000659 
  5. Ansary TM, Hossain MR, Kamiya K, Komine M, Ohtsuki M (2021) Inflammatory molecules associated with ultraviolet radiation-mediated skin aging. Int J Mol Sci 22: 3974-3988. doi: 10.3390/ijms22083974 
  6. Jin YJ, Ji Y, Jang YP, Choung SY (2021) Acer tataricum subsp. ginnala inhibits skin photoaging via regulating MAPK/AP-1, NF-κB, and TGFβ/Smad signaling in UVB-irradiated human dermal fibroblasts. Molecules 26: 662. doi: 10.3390/molecules26030662 
  7. de Jager TL, Cockrell AE, du Plessis SS (2017) Ultraviolet light induced generation of reactive oxygen species. Adv Exp Med Biol 996: 15-23. doi: 10.1007/978-3-319-56017-5_2 
  8. Choi SJ, Lee SN, Kim K, Joo DH, Shin S, Lee J, Lee HK, Kim J, Kwon SB, Kim MJ, Ahn KJ, An IS, An S, Cha HJ (2016) Biological effects of rutin on skin aging. Int J Mol Med 38: 357-363. doi: 10.3892/ijmm.2016.2604 
  9. Quan T, He T, Kang S, Voorhees JJ, Fisher GJ (2004) Solar ultraviolet irradiation reduces collagen in photoaged human skin by blocking transforming growth factor-β type II receptor/Smad signaling. Am J Pthol 165: 741-751. doi: 10.1016/S0002-9440(10)63337-8 
  10. Chandra S, Qureshi S, Chopra D, Shukla S, Patel SK, Singh J, Ray RS (2022) UVR-induced phototoxicity mechanism of methyl N-methylanthranilate in human keratinocyte cell line. Toxicol in Vitro 80: 105322. doi: 10.1016/j.tiv.2022.105322 
  11. Bae JT, Ko HJ, Kim GB, Pyo HB, Lee GS (2012) Protective effects of fermented citrus unshiu peel extract against ultraviolet-a-induced photoageing in human dermal fibrobolasts. Phytother Res 26: 1851-1856. doi: 10.1002/ptr.4670 
  12. Mohamed MAA, Jung M, Lee SM, Lee TH, Kim J (2014) Protective effect of Disporum sessile D.Don extract against UVB-induced photoaging via suppressing MMP-1 expression and collagen degradation in human skin cells. J Photochem Photobiol B 133: 73-79. doi: 10.1016/j.jphotobiol.2014.03.002 
  13. Kim H il, Jeong YU, Kim JH, Park YJ (2018) 3,5,6,7,8,3',4'-heptamethoxyflavone, a citrus flavonoid, inhibits collagenase activity and induces type I procollagen synthesis in HDFn cells. Int J Mol Sci 19: 620. doi: 10.3390/ijms19020620 
  14. Webb AH, Gao BT, Goldsmith ZK, Irvine AS, Saleh N, Lee RP, Lendermon JB, Bheemreddy R, Zhang Q, Brennan RC, Johnson D, Steinle JJ, Wilson MW, Morales-Tirado VM (2017) Inhibition of MMP-2 and MMP-9 decreases cellular migration, and angiogenesis in in vitro models of retinoblastoma. BMC Cancer 17: 434. doi: 10.1186/s12885-017-3418-y 
  15. Quan T, He T, Voorhees JJ, Fisher GJ (2001) Ultraviolet irradiation blocks cellular responses to transforming growth factor-β by down-regulating its type-II receptor and inducing Smad7. J Biol Chem 276: 26349-26356. doi: 10.1074/jbc.M010835200 
  16. Nam EJ, Yoo G, Lee JY, Kim M, Jhin C, Son YJ, Kim SY, Jung SH, Nho CW (2020) Glycosyl flavones from Humulus japonicus suppress MMP-1 production via decreasing oxidative stress in UVB irradiated human dermal fibroblasts. BMB Rep 53: 379-384. doi: 10.5483/BMBRep.2020.53.7.253 
  17. Hyun HB, Hyeon HJ, Kim SC, Go B, Yoon SA, Jung YH, Ham YM (2021) Anti-melanogenesis effects of Schizophragma hydrangeoides leaf ethanol extracts via downregulation of tyrosinase activity. Korean J Plant Res 34: 510-516. doi: 10.7732/KJPR.2021.34.6.510 
  18. Venditti A, Maggi F, Vittori S, Papa F, Serrilli AM, Cecco MD, Ciaschetti G, Mandrone M, Poli F, Bianco A (2015) Antioxidant and α-glucosidase inhibitory activities of Achillea tenorii. Pharm Biol 53: 1505-1510. doi: 10.3109/13880209.2014.991833 
  19. Hasler A, Gross G-A, Meier B, Sticher O (1992) Complex flavonol glycosides from the leaves of Ginkgo biloba. Phytochem 31: 1391-1394. doi: 10.1016/0031-9422(92)80298-S 
  20. Bilia AR, Ciampi L, Mendez J, Morelli I (1996) Phytochemical investigations of Licania genus. flavonoids from Licania pyrifolia. Pharm Acta Helv 71: 199-204. doi: 10.1016/0031-6865(96)00009-X 
  21. Chang SW, Kim KH, Lee IK, Ryu SY (2009) Phytochemical constituents of Bistorta manshuriensis. Nat Prod Sci 15: 234-240 
  22. Oh JH, Kim J, Karadeniz F, Kim HR, Park SY, Seo Y, Kong CS (2021) Santamarine shows anti-photoaging properties via inhibition of MAPK/AP-1 and stimulation of TGF-β/Smad signaling in UVA-irradiated HDFs. Molecules 26. doi:10.3390/molecules26123585 
  23. Oh JH, Karadeniz F, Kong CS, Seo Y (2020) Antiphotoaging effect of 3,5-dicaffeoyl-epi-quinic acid against UVA-induced skin damage by protecting human dermal fibroblasts in vitro. Int J Mol Sci 21: 1-12. doi:10.3390/ijms21207756 
  24. Hwang E, Gao W, Xiao YK, Ngo HTT, Yi TH (2019) Helianthus annuus L. flower prevents UVB-induced photodamage in human dermal fibroblasts by regulating the MAPK/AP-1, NFAT, and Nrf2 signaling pathways. J Cell Biochem 120: 601-612. doi: 10.1002/jcb.27417 
  25. Ryu TK, Roh E, Shin HS, Kim JE (2022) Inhibitory effect of lotusine on solar UV-induced matrix metalloproteinase-1 expression. Plants 11: 773. doi:10.3390/plants11060773 
  26. Lee SH, Yang JH, Park YK, Han JJ, Chung GH, Hahm DH, Choi HD (2013) Protective effect and mechanism of phosphatidylserine in UVB-induced human dermal fibroblasts. Eur J Lipid Sci Technol 115: 783-790. doi: 10.1002/ejlt.201200086 
  27. Kim YJ, Lee EH, Cho EB, Kim DH, Kim BO, Kang I, Jung HY, Cho YJ (2019) Protective effects of galangin against UVB irradiation-induced photo-aging in CCD-986sk human skin fibroblasts. Appl Biol Chem 40. doi: 10.1186/s13765-019-0443-3 
  28. Zhao P, Alam MB, An H, Choi HJ, Cha YH, Yoo CY, Kim HH, Lee SH (2018) Antimelanogenic effect of an Oroxylum indicum seed extract by suppression of MITF expression through activation of MAPK signaling protein. Int J Mol Sci 19: 760. doi: 10.3390/ijms19030760 
  29. Kim DE, Chang BY, Ham SO, Kim YC, Kim SY (2020) Neobavaisoflavone inhibits melanogenesis through the regulation of Akt/GSK-3β and MEK/ERK pathways in B16F10 cells and a reconstructed human 3D skin model. Molecules 25. doi: 10.3390/molecules25112683 
  30. Hong JA, Bae D, Oh KN, Oh DR, Kim Y, Kim Y, Im SJ, Choi E, Lee S, Kim M, Jeong C, Choi CY (2022) Protective effects of Quercus acuta Thunb. fruit extract against UVB-induced photoaging through ERK/AP-1 signaling modulation in human keratinocytes. BMC Complement Med Ther 22. doi: 10.1186/s12906-021-03473-1 
  31. Oh JH, Joo YH, Karadeniz F, Ko J, Kong CS (2020) Syringaresinol inhibits UVA-induced MMP-1 expression by suppression of MAPK/AP-1 signaling in HaCaT keratinocytes and human dermal fibroblasts. Int J Mol Sci 21: 3981. doi:10.3390/ijms21113981 
  32. Kim M, Park YG, Lee HJ, Lim SJ, Nho CW (2015) Youngiasides A and C isolated from Youngia Denticulatum inhibit UVB-induced MMP expression and promote type I procollagen production via repression of MAPK/AP-1/NF-κB and activation of AMPK/Nrf2 in HaCaT cells and human dermal fibroblasts. J Agric Food Chem 63: 5428-5438, doi: 10.1021/acs.jafc.5b00467 
  33. Darwin JP, Aleksander LS, Shi-Wu Li (1998) Procollagen N-proteinase and procollagen C-proteinase. Two unusual metalloproteinases that are essential for procollagen processing probably have important roles in development and cell signaling. Matrix Biol 16: 399-408. doi: 10.1016/S0945-053X(98)90013-0