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http://dx.doi.org/10.3839/jabc.2022.036

Protective effects of quercetin-3-glucosyl-(1-2)-rhamnoside from Schizophragma hydrangeoides leaves on ultraviolet A-induced photoaging in human dermal fibroblasts  

So Yeon Oh (Biodiversity Research Institute, Jeju Technopark)
Sung Chun Kim (Biodiversity Research Institute, Jeju Technopark)
Ho Bong Hyun (Biodiversity Research Institute, Jeju Technopark)
Hyejin Hyeon (Biodiversity Research Institute, Jeju Technopark)
Boram Go (Biodiversity Research Institute, Jeju Technopark)
Yong-Hwan Jung (Biodiversity Research Institute, Jeju Technopark)
Young-Min Ham (Biodiversity Research Institute, Jeju Technopark)
Publication Information
Journal of Applied Biological Chemistry / v.65, no.4, 2022 , pp. 277-286 More about this Journal
Abstract
Schizophragma hydrangeoides (S. hydrangeoides) is a vine endogenous to Jeju Island and Ulleungdo, where it grows attached to the foothills and rock surfaces. Previous research has mostly focused on the whitening effect of S. hydrangeoides leaf extract. In this study, we investigated S. hydrangeoides leaf extract further, and detected four phytochemicals in the extract: chlorogenic acid, quercetin-3-O-glucosyl-(1-2)-rhamnoside, quercetin-3-O-xylosyl-(1-2)-rhamnoside, and quercitrin. We pretreated human dermal fibroblast (HDFn) cells with previously established concentrations of the four compounds for 1 h before ultraviolet A (UVA) irradiation. Among the four compounds, quercetin-3-O-glucosyl-(1-2)-rhamnoside (Q-3-GR) best inhibited matrix metalloproteinase-1 (MMP-1) levels. Thus, we investigated the protective effects of Q-3-GR on photoaging and its underlying mechanisms. Q-3-GR significantly reduced MMP-1 production and inhibited MMP-1 protein expression in UVA-irradiated HDFn cells. Furthermore, Q-3-GR increased procollagen type I production and protein expression. Q-3-GR exerted its anti-photoaging effects by downregulating the mitogen-activated protein kinase/ activator protein-1 signaling pathway, and upregulating the transforming growth factor-β/Smad signaling pathway. Thus, S. hydrangeoides leaf-derived Q-3-GR is a potential potent cosmetic ingredient for UV-induced skin aging.
Keywords
Collagen; Matrix Metalloproteinase-1; Mitogen-activated Protein Kinases;
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Times Cited By KSCI : 7  (Citation Analysis)
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