• 한국산학기술학회논문지
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• 제14권11호
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• pp.5385-5391
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• 2013

온라인을 기반한 제품의 구매가 활성화 됨에 따라, 소비자들의 제품 디자인에 대한 사실적이고 정확한 정보를 요구하고 있다. 본 연구에서는 제품의 형상정보(3D mesh)와 색/질감정보(image)를 이용하여 텍스쳐 맵핑된 실사적 3차원 모델을 생성하는 효율적인 방법을 제안한다. 3 차원 형상정보에 대응하는 이미지 상의 텍스쳐 좌표 관계를 구하기 위해 오브젝트 좌표계와 카메라 좌표계 사이의 변환행렬, 카메라의 초점거리, 카메라 CCD 와 프레임상의 이미지 사이의 종횡비를 파라미터로 하는 2D-3D 정합을 수행한다. 이러한 2D-3D 정합에 있어 발생하는 연산의 복잡도와 비선형도를 낮추기 위하여, 카메라 내부파라미터 검정단계, 임의의 회전행렬에 대한 임시적 최적 이동 벡터 (TOTV), 회전행렬에 대한 비선형 최적화 단계로 접근한다. 제안하는 방법의 유용성을 시현하기 위해 3차원 컬러 측정기로는 색외관을 획득하기 힘든 메탈릭 페인트 재질로 이루어진 제품에 적용한 구현결과를 보인다.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2007년도 정기 학술대회 논문집
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• pp.577-582
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• 2007

A variational formulation for plane elasticity problems is derived based on an isogeometric approach. The isogeometric analysis is an emerging methodology such that the basis functions in analysis domain arc generated directly from NURBS (Non-Uniform Rational B-Splines) geometry. Thus. the solution space can be represented in terms of the same functions to represent the geometry. The coefficients of basis functions or the control variables play the role of degrees-of-freedom. Furthermore, due to h-. p-, and k-refinement schemes, the high order geometric features can be described exactly and easily without tedious re-meshing process. The isogeometric sensitivity analysis method enables us to analyze arbitrarily shaped structures without re-meshing. Also, it provides a precise construction method of finite element model to exactly represent geometry using B-spline base functions in CAD geometric modeling. To obtain precise shape sensitivity, the normal and curvature of boundary should be taken into account in the shape sensitivity expressions. However, in conventional finite element methods, the normal information is inaccurate and the curvature is generally missing due to the use of linear interpolation functions. A continuum-based adjoint sensitivity analysis method using the isogeometric approach is derived for the plane elasticity problems. The conventional shape optimization using the finite element method has some difficulties in the parameterization of boundary. In isogeometric analysis, however, the geometric properties arc already embedded in the B-spline shape functions and control points. The perturbation of control points in isogeometric analysis automatically results in shape changes. Using the conventional finite clement method, the inter-element continuity of the design space is not guaranteed so that the normal vector and curvature arc not accurate enough. On tile other hand, in isogeometric analysis, these values arc continuous over the whole design space so that accurate shape sensitivity can be obtained. Through numerical examples, the developed isogeometric sensitivity analysis method is verified to show excellent agreement with finite difference sensitivity.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.356-363
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• 2016

Human papillomavirus (HPV), a non-enveloped, double-stranded DNA tumor virus, is a primary etiological agent of cervical cancer development. As a potential tool for prophylactic vaccination, the development of virus-like particles (VLPs) containing the HPV16 L1 capsid protein is highly desired. In this study, we developed a high-level expression system of the HPV16 L1 in Escherichia coli for the purpose of VLP development. The native gene of HPV16 L1 has many rare codons that cause the early termination of translation and result in the production of truncated forms. First, we optimized the codon of the HPV16 L1 gene to the preferable codons of E. coli, and we succeeded in producing the full-size HPV16 L1 protein without early termination. Next, to find the best host for the production of HPV16 L1, we examined a total of eight E. coli strains, and E. coli BL21(DE3) with the highest yield among the strains was selected. With the selected host-vector system, we did a fed-batch cultivation in a lab-scale bioreactor. Two different feeding solutions (complex and defined feeding solutions) were examined and, when the complex feeding solution was used, a 6-fold higher production yield (4.6 g/l) was obtained compared with that with the defined feeding solution.

• 한국지능시스템학회논문지
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• 제11권3호
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• pp.223-230
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• 2001

자기구성 지도는 주어진 입력에 대해 올바른 출력 값이 제공되지 않는 비교사 방식으로 학습된다. 또한, 반응하는 순서나 위치를 통해 위상이 보존(topology preserving)되는 특성을 가지고 있어 많은 분야에 응용되고 있다. 그러나, 자기 구성지도는 학습이 되기 전에 위상을 미리 고정시켜야 하기 때문에 실제 문제에 적용하기 어렵다는 단점을 가지고 있다. 구조 적응형 자기구성 지도는 자기구성 지도의 고정된 구조 때문에 발생하는 문제를 해결하기 위해 지도의 구조를 학습 중에 적절하게 변경시킨다. 이때, 변화된 구조의 가중치를 어떻게 초기화시킬 것인가 하는 것이 또한 중요한 문제이다. 이 논문에서는 구조 적응형 자기구성 지도 모델에서 유전자 알고리즘을 이용하여 분화된 노드의 가중치를 결정하는 방법을 제안한다. 이 방법은 기존의 구조 적응형 자기구성 지도보다 다소 높은 인식률을 보였고, 숫자 별 인식률 편차를 줄일 수 있었다. 오프라인 필기 숫자 데이터로 실험한 결과, 제안한 방법이 유용함을 알 수 있었다.

• 한국전자파학회논문지
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• 제10권3호
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• pp.419-429
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• 1999

전파의 유효 수신 신호 도달영역을 정확히 식별하는 것은 기지국 최적화를 이룩하는데 있어 가장 중요한 요소중 하나이다. 서울 도심 지역과 같이 고층건물이 밀집되어 있고, 넓은 도로와 좁은 도로가 불규칙적으로 배치되어 있으며, 고개와 강 등이 혼재된 지역에서도 높은 정확도를 갖는 전파손실 예측모델을 소개한다. 이 모델은 기 측정된 필드데이터로 훈련된 신경회로망을 기반으로 한다. 전파손실에 영향을 주는 가장 기본적인 변수들은 3차원 DEM 데이터와 벡터 데이터로부터 추출하여 신경회로망의 입력으로 사용한다. 학습이 완료된 신경회로망은 전파손실 모델의 근사함수이며, 학습에 사용된 필드 측정데이터에 포함되지 않은 타지역에서도 정확한 예측이 가능한 일반화 능력을 갖는다. 서울 도심 지역의 실제 서비스 환경에 대한 비교 실험결과를 통해 제안하는 모델의 우수성을 보인다.

• 한국게임학회 논문지
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• 제18권1호
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• pp.125-134
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• 2018

본 논문에서는 시간에 따라 연속적으로 변하는 $3{\times}3$ 행렬의 회전 성분을 효율적으로 추출하는 실용적인 방법을 제안한다. 이는 물리기반 동적 변형을 위하여 널리 사용되는 공회전 유한 요소법이나 형상 맞춤 변형에서 매우 중요한 기술이다. 최근 극분해를 사용하는 시간 독립적인 기존 방법들과 달리 회전행렬 추출을 물리적으로 공식화한 후, 점진적 회전 표현법을 이용하는 반복법이 제안되었다. 본 논문에서는 점진적 회전 벡터의 최대 회전각을 ${\pi}/2$ 이내로 제한함으로써 반복 횟수를 줄이는 최적화 기법을 개발한다. 사실적인 동적 변형 시뮬레이션에서는 충분히 작은 시간 간격을 사용하기 때문에 이러한 제한은 실용적으로 문제가 되지 않는다. 다양한 실험을 통해 제안된 방법의 효율성 및 실용성을 보인다.

• Mycobiology
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• 제49권5호
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• pp.491-497
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• 2021

An endolichenic fungus Xylaria grammica EL000614 produces grammicin, a potent nematicidal pyrone derivative that can serve as a new control option for root-knot nematodes. We optimized an Agrobacterium tumefaciens-mediated transformation (ATMT) protocol for X. grammica to support genetic studies. Transformants were successfully generated after co-cultivation of homogenized young mycelia of X. grammica with A. tumefaciens strain AGL-1 carrying a binary vector that contains the bacterial hygromycin B phosphotransferase (hph) gene and the eGFP gene in T-DNA. The resulting transformants were mitotically stable, and PCR analysis showed the integratin of both genes in the genome of transformants. Expression of eGFP was confirmed via fluorescence microscopy. Southern analysis showed that 131 (78.9%) out of 166 transformants contained a single T-DNA insertion. Crucial factors for producing predominantly single T-DNA transformants include 48 h of co-cultivation, pretreatment of A. tumefaciens cells with acetosyringone before co-cultivation, and using freshly prepared mycelia. The established ATMT protocol offers an efficient tool for random insertional mutagenesis and gene transfer in studying the biology and ecology of X. grammica.

• Journal of Veterinary Science
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• 제22권6호
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• pp.76.1-76.15
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• 2021

Background: The development of a vaccine for Jembrana disease is needed to prevent losses in Indonesia's Bali cattle industry. A DNA vaccine model (pEGFP-C1-tat) that requires a functional delivery system will be developed. Polylactic-co-glycolic acid (PLGA) may have potential as a delivery system for the vaccine model. Objectives: This study aims to evaluate the in vitro potential of PLGA as a delivery system for pEGFP-C1-tat. Methods: Consensus and codon optimization for the tat gene was completed using a bioinformatic method, and the product was inserted into a pEGFP-C1 vector. Cloning of the pEGFP-C1-tat was successfully performed, and polymerase chain reaction (PCR) and restriction analysis confirmed DNA isolation. PLGA-pEGFP-C1-tat solutions were prepared for encapsulated formulation testing, physicochemical characterization, stability testing with DNase I, and cytotoxicity testing. The PLGA-pEGFP-C1-tat solutions were transfected in HeLa cells, and gene expression was observed by fluorescent microscopy and real-time PCR. Results: The successful acquisition of transformant bacteria was confirmed by PCR. The PLGA:DNA:polyvinyl alcohol ratio formulation with optimal encapsulation was 4%:0.5%:2%, physicochemical characterization of PLGA revealed a polydispersity index value of 0.246, a particle size of 925 nm, and a zeta potential value of -2.31 mV. PLGA succeeded in protecting pEGFP-C1-tat from enzymatic degradation, and the percentage viability from the cytotoxicity test of PLGA-pEGFP-C1-tat was 98.03%. The PLGA-pEGFP-C1-tat demonstrated luminescence of the EGFP-tat fusion protein and mRNA transcription was detected. Conclusions: PLGA has good potential as a delivery system for pEGFP-C1-tat.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권4호
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• pp.1765-1794
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• 2019

Genetic Programming (GP) is an intelligence technique whereby computer programs are encoded as a set of genes which are evolved utilizing a Genetic Algorithm (GA). In other words, the GP employs novel optimization techniques to modify computer programs; imitating the way humans develop programs by progressively re-writing them for solving problems automatically. Trial programs are frequently altered in the search for obtaining superior solutions due to the base is GA. These are evolutionary search techniques inspired by biological evolution such as mutation, reproduction, natural selection, recombination, and survival of the fittest. The power of GAs is being represented by an advancing range of applications; vector processing, quantum computing, VLSI circuit layout, and so on. But one of the most significant uses of GAs is the automatic generation of programs. Technically, the GP solves problems automatically without having to tell the computer specifically how to process it. To meet this requirement, the GP utilizes GAs to a "population" of trial programs, traditionally encoded in memory as tree-structures. Trial programs are estimated using a "fitness function" and the suited solutions picked for re-evaluation and modification such that this sequence is replicated until a "correct" program is generated. GP has represented its power by modifying a simple program for categorizing news stories, executing optical character recognition, medical signal filters, and for target identification, etc. This paper reviews existing literature regarding the GPs and their applications in different scientific fields and aims to provide an easy understanding of various types of GPs for beginners.

• Genomics & Informatics
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• 제20권1호
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• pp.11.1-11.20
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• 2022

Vibrio harveyi belongs to the Vibrio genus that causes vibriosis in marine and aquatic fish species through double-stranded DNA virus replication. In humans, around 12 Vibrio species can cause gastroenteritis (gastrointestinal illness). A large amount of virus particles can be found in the cytoplasm of infected cells, which may cause death. Despite these devastating complications, there is still no cure or vaccine for the virus. As a result, we used an immunoinformatics approach to develop a multi-epitope vaccine against most pathogenic hemolysin gene of V. harveyi. The immunodominant T- and B-cell epitopes were identified using the hemolysin protein. We developed a vaccine employing three possible epitopes: cytotoxic T-lymphocytes, helper T-lymphocytes, and linear B-lymphocyte epitopes, after thorough testing. The vaccine was developed to be antigenic, immunogenic, and non-allergenic, as well as having a better solubility. Molecular dynamics simulation revealed significant structural stiffness and binding stability. In addition, the immunological simulation generated by computer revealed that the vaccination might elicit immune reactions in the actual life after injection. Finally, using Escherichia coli K12 as a model, codon optimization yielded ideal GC content and a higher codon adaptation index value, which was then included in the cloning vector pET2+ (a). Altogether, our experiment implies that the proposed peptide vaccine might be a good option for vibriosis prophylaxis.